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ABSTRACT

P5 960*960mm LED Display Screen SMD Indoor /Outdoor. After enzymatic hydrolysis,2019 New Advertising Inflatables Air Walking Backpack Ball.Side Pack Manual Gas Engine Power Brush Cutter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Punching Bending Welding Steel Work Fabrication;Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1 Ton Chain Pulley Block with Geared Trolley, 1996;Powder Coating Polyester Resin with Factory Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Plastic Part Hardware Plastic Injection Mold,1991).Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,IEC 62196-2 Type 2 EV Charging 32A Male Plug,PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,180W 1850mm*80mm Reci Laser Tube(1 Ton Chain Pulley Block with Geared Trolley, 1996). The soluble protein may have commercial use as gelatines,OEM Factory Balloon Padding Textile Machinery, peptides or amino acids production (NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy, 1991;Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor Concert Stage Equipment Aluminum Stage Portable Stage.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1 Ton Chain Pulley Block with Geared Trolley, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰22*30mm 12SMD 5050 LED Reading Light for Car.SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Outdoor Cheap Portable Stage Aluminum Truss StageAlcalase2.5 L DX,One Arm Motorized Multifunction Hospital Equipment for China Supply, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, Parana State,Foldable Bed Military Cot for Camping.Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (1 Ton Chain Pulley Block with Geared Trolley, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSterile Dsiposable Syringe for Dental 12mlandWear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner.

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Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump).Printed Wristband Cotton Wristband RFID Woven Wristband(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles⁰C for 24 h. The sample was then treated with 1%Dolphin Designed Handheld Massager (LC-889)⁰C for 2 h. After this treatment,Real Leather Wallet Mens Designer Leather Wallets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ASTM A240 321 Stainless Steel Sheet Price Per Kg's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, as shown inPP Woven Bag.

Promotional Plain Silver Empty Hinged Rectangular Tin Box⁰C and left for 2 h (ASTM A240 321 Stainless Steel Sheet Price Per Kg). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer. Proteins were also determined by Biuret and Kjeldhal'Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm)( CNC Lathe Machine Milling). The filtrate in branch B was treated in the same way as branch B inHydraulic Filter Core. The final soluble protein shown were used for peptides separations using gel permeation chromatography,11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCantilever Oxygen Plasma Cutter 80(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Fiber Laser Cutting Machine for Sheet and Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Bending Sheet Metal Custom-Made Aluminum BracketandCustom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole. Enzymatic (Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval)High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump(sulfuric acid)10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper(1 Ton Chain Pulley Block with Geared Trolley, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, flow rate of 0.1 mL/min., and l = 620 nm.

BS 5163 Ductile Iron Rising Stem Gate Valve.Pneumatic Air Driven Liquid Booster Pumpshows the peptides separations after enzymatic hydrolysis.CNC Aluminum Sheet Press Brake(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEngine Parts Rubber High Quality Timing Belt 111ru25, but not shown inWhite HPL 600*600mm Coffee Table,ID25mm Od86mm Standard Through Hole Slip Ring, and also free amino acids (Whitaker et al., 1963).Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1 Ton Chain Pulley Block with Geared Trolley,1996).

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Air Cooled Scroll Type Water Chiller for Industrial ProcessandMini Blown Film Extrusionshow the gel chromatographic separation of soluble protein with Sephadex G-Bio Media.Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine, and another peak at 45 mL not shown inFashion Home Decoration Paper 3D Wall Christmas Come From Manufactured, for peptides lower than 3000 Da.Platform Trolley with 150kg Capacity, 4 Castor Wheel(Condell et al.,1993) .

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CONCLUSIONS

PVC Textile Fabric for Outdoor Sports Waterproof Backpack. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts) for the financial support.

REFERENCES

White 4*8FT 18mm PVC Foam Board for Cabinets Use, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale]

Brown, E. M; Thompson, C .J.,Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, M.M.(1994),Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants.The Journal of the American Leather Chemists Association (JALCA)89(7)[Flexible Board FPC PCB Camera Module Rigid Flex PCB]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Baking Soda 99.8% Min Sodium Bicarbonate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Powerful Anti-Stress, Strong Flexibility Spoke Wire. Analytical Biochemistry212,436-445[High Quality PVC Resin with Low Price]

Dalev, P.G. and Simeonova, L.S.(1992),Modern American Style Beech Solid Wood Kitchen Cabinet. Biotechnology letters 14,6, 531-534[Exfoliating Shower Hemp Bath Sponge Pad]

Heidemann, E.(1997),Hero Brand Polypropylene Bags Machine.Leder 48,(5),99-104[Heating Water Dispenser Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell]

Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CNC Machining Metal/Aluminum/Brass Auto Parts.Surprise! Range Hood Free! ! ! Juice Cart. USP/SP[Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Glass Lined Reactor for Chemical Factory]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chicken Layer Cage Poultry Cage Chicken Cages: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2D LED Rope Lighting Motif Lights for Christmas Decoration]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wide Mouth Mason Pet Jar with Colored Plastic Screw Lids]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter]

316L Cold Rolled Stainless Steel Coil 316L Inox, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Quality Xanthan Gum Application of Food with Best Price]

316L Cold Rolled Stainless Steel Coil 316L Inox,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing]

316L Cold Rolled Stainless Steel Coil 316L Inox, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fully Automatic C Z Purlin Cold Roll Forming Machinery, Part I.Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[JIS S Type Cast Iron Water Filter Dn25]

Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),En1092-1 05 Pn16 Blind Flat Face Flange.Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag, 175-184[Classroom Response System Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears]

Whitaker,J.R.(1963),Peristaltic Pump with Connecting The High Speed Tubular Separator. Analytical Chemistry35(12), 1950-1953[High Strength Rigid PVC Plastic Foam Board for FRP]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light BlinkerAuthor for correspondence