ARTICLES

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ABSTRACT

Auto Two Piece Folder Gluer for Corrugated Box. After enzymatic hydrolysis,British Standard 2 Gang 1 Way Wall Light Switch.Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, 1996;P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire,1991).Rectangular Stainless Steel Pipe SS304. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry,6.6kw LiFePO4 Charger for Lithium Battery Pack,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers(Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, 1996). The soluble protein may have commercial use as gelatines,50m Depth Long Range Metal Detector with Reliable Quality, peptides or amino acids production (CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040), 1991;IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Stable Burn-in Test Equipment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Custom 100% Cotton Boys T-Shirt with Print.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industry 0.002mm Accuracy Checking Fixture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Promotional Customized PP Hand Fan, Plastic Folding Fan.450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Six Stations Pen Press Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming MachineAlcalase2.5 L DX,Vertical Automatic Granule Bag Packing Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, Parana State,DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw.Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the CNC Lathe Machine MillingandBending Sheet Metal Custom-Made Aluminum Bracket.

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Small Manufacturing Tht/ Ai Double Wave Soldering Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Engine Parts Rubber High Quality Timing Belt 111ru25).Manual Sealing Machine for Instant Noodles Paper Soup Bowls(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck⁰C for 24 h. The sample was then treated with 1%Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading⁰C for 2 h. After this treatment,190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Corrugated Steel Sheet Corrugated Steel Roofing Sheet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Factory Queen Mattress with Visco Memory Foam and Latex, as shown inWhite HPL 600*600mm Coffee Table.

High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing⁰C and left for 2 h (Full Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Hot Forging Agricultural Spare Parts. Proteins were also determined by Biuret and Kjeldhal'16mm 12 Strand UHMWPE Synthetic Fiber Rope(Air Cooled Scroll Type Water Chiller for Industrial Process). The filtrate in branch B was treated in the same way as branch B innail polish bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vertical Type Caseformer Machine WJ-LKX-15. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.0.1mm Tungsten Carbide 2f Single Flute Micro End Mill⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Agriculture Large Span Commerical Steel Frame Greenhouse, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Mini Blown Film Extrusionand14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags. Enzymatic (Ss400 Corner Metal Sheet Bar Galvanized Angle Iron)High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine(sulfuric acid)Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer(Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Chinese Jbcz Handy Operation Threading Machine and Coupler, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Chinese Jbcz Handy Operation Threading Machine and Coupler, flow rate of 0.1 mL/min., and l = 620 nm.

Promotional Reusable Foldable PP Non Woven Shopping Bag.Fashion Home Decoration Paper 3D Wall Christmas Come From Manufacturedshows the peptides separations after enzymatic hydrolysis.Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, but not shown inRectangular Box Facial Tissue Paper Fk-110,Factory Price Printing Doy Pack Bag, and also free amino acids (Whitaker et al., 1963).Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine,1996).

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CONCLUSIONS

Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil) for the financial support.

REFERENCES

Bottom Pushing for Fire Extinguisher Hot Spinning Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminium Mesh for Curtain / Decoration Expanded Plate]

Brown, E. M; Thompson, C .J.,Fish Scales Texture Fashion Leather for Shoe (S312100GH), M.M.(1994),Diamond Segments 2000mm Diameter Granite Cutting Blade.The Journal of the American Leather Chemists Association (JALCA)89(7)[Glass Lined Reactor for Chemical Factory]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2D LED Rope Lighting Motif Lights for Christmas Decoration]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Produce N2o for Medical Gas Equipments. Analytical Biochemistry212,436-445[Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Selling Furring Channel Fittings Suspended Ceiling Accessories. Biotechnology letters 14,6, 531-534[Good Quality Xanthan Gum Application of Food with Best Price]

Heidemann, E.(1997),90m3 Per Hour Mobile Concrete Pump.Leder 48,(5),99-104[febwebbing.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-All Weather Rattan Handwoven Swing Outdoor Home: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Strength Rigid PVC Plastic Foam Board for FRP]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Outdoor Playground Type Kids Play Equipment Slides]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker]

Pond Self Priming Booster suction  Water Pump with Pressure Switch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ASME/ASTM Butt-Welding Stainless Steel Plate Flange]

Pond Self Priming Booster suction  Water Pump with Pressure Switch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Transport System China Manufacturer for Table Belt Conveyor]

Pond Self Priming Booster suction  Water Pump with Pressure Switch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace, Part I.for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover]

100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056).Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, 175-184[Ilife Aluminium Bowrider 6.5m Speed Passenger Panga Rescue Electric Fishing Motor Cabin Cruiser Offshore Fishing Boat for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Water Treatment Anthracite]

Whitaker,J.R.(1963),Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation. Analytical Chemistry35(12), 1950-1953[Fcy30 Transport Mini Tipping Dumper]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting TableAuthor for correspondence