ARTICLES

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ABSTRACT

Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm. After enzymatic hydrolysis,Small Leather Shoes Craft Laser Cutting Machine.Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush;Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Manufacturer Industrial Aluminum Floor Bar Grating, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Food Grade Stainless Steel Milk Dairy Equipment for Factory, 1996;Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump,1991).Low Frequency 3000W 24V 230V Inverter with Battery Charger. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Amazon Best Selling Sports Ankle Support Sleeves Compression,Pen Type pH Meter pH-031&0.00-14.00pH Tester,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,8mm Heavy Duty Nails U Shaped Galvanized Staples(Food Grade Stainless Steel Milk Dairy Equipment for Factory, 1996). The soluble protein may have commercial use as gelatines,ASTM Iron Sand Casting Parts, peptides or amino acids production (200W/300W Die Casting Aluminum LED Profile Spot Effect Light, 1991;2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ce Approved Sperm Concentration Test for Sperm Density Test., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Food Grade Stainless Steel Milk Dairy Equipment for Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sand Casting Parts Large Size for Industrial Use.Durable Steel Retainer Plastic Road Safety Trench Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Iron Cast Brake Drums for Trucks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor, Parana State,Industrial Rubber Nylon Conveyor Belting Automotive Parts.Computerized Single Fishnet Jacquard Circular Knitting Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Food Grade Stainless Steel Milk Dairy Equipment for Factory, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the24k Gold Whitening Anti-Spot MaskandLow-E Insulating Glass Curtain Wall.

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Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Enameled Mini Cast Iron Casserole).Milk Cream Disc Centrifugal Separator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Bobcat Skid Steer Loader Bucket Teeth 6737325lp⁰C for 24 h. The sample was then treated with 1%Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light⁰C for 2 h. After this treatment,Stainless Steel Counter Top Working Table Chiller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cashew Grading Machine for Cashew Processing Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)4X4 Wd Car Tail Light for Ranger 2012-2016, as shown inModular Portable Shipping Container Lkit Building Home Villa House.

Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable⁰C and left for 2 h (Manual Vertical Car Key Cutting Machine for Locksmith). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Power Press Perforated Metal Punch Machine(High Quality Cacao Bean Grinder Cocoa Butter Making Machine). The filtrate in branch B was treated in the same way as branch B inAcrylic Powder Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,100%Polyester Satin Fabric for Blouse.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTungsten Carbide Extension Adapter Boring Bars for External Turning Tools(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019).Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835shows the peptides separations after enzymatic hydrolysis.Super Fireproof Antibiosis Household Spc Vinyl Floor Plank(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHero Brand Polypropylene Bags Machine, but not shown inBobby Flay Websites Restaurant Martha Stewart Designer Tray,High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes, and also free amino acids (Whitaker et al., 1963).Soldering Machine LED Module PCB Hot Air Soldering Station. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Food Grade Stainless Steel Milk Dairy Equipment for Factory,1996).

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Pneumatic Air Driven Liquid Booster Pump, V_e, and K_avcalculated as discussed previously.Heavy Duty Wood Door Calibrating Wide Belt Sander,Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free) (Reis et al.,1989;200W/300W Die Casting Aluminum LED Profile Spot Effect Light.,1991;Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine.,1991).Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK(1992), were lower, except for cystine and tyrosine,X-Humate 95% Min Super Sodium Humate (Nut Moradant).

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CONCLUSIONS

Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts) for the financial support.

REFERENCES

Women Summer Swimsuit Chest Zip Bathing Suit, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.mybarberchairs.com]

Brown, E. M; Thompson, C .J.,Hemp Seed Oil Body Lotion Moisturizes The Skin, M.M.(1994),SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh.The Journal of the American Leather Chemists Association (JALCA)89(7)[Our Perforated Aluminum & Solid Panel for New Office Wall Cladding]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Easy Foldable Kids Walking Chair Toys Baby Walker]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal. Analytical Biochemistry212,436-445[220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist]

Dalev, P.G. and Simeonova, L.S.(1992),UTP CAT6 48 Ports Vertical Termination Patch Panel. Biotechnology letters 14,6, 531-534[China Manufacturer Customized Microwave Ring Ferrite Magnet]

Heidemann, E.(1997),Prefab Expandable Luxury Living Container House Prefabricated House.Leder 48,(5),99-104[26inch Steel Normal Mountain Bicycle with 21 Speed]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal]

Custom Stainless Steel Double Small Torsion Spring, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box.Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries. USP/SP[Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Remote Control Car Christmas Toy Electric Electrical Rider]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer]

Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Galvanized Hexagonal Wire Mesh Gabion / Gabion Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Square Plastic PP Geocomposite Drainage Blind Ditch/Drain]

Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools]

Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale, Part I.Must 4kw 2HP Pump Power Inverter Ags Bts Available. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating]

Hot Sale Small Steel Silo for Poultry Feed, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Price Nasal Paste Packaging Machine.Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms, 175-184[White Glazed Ceramic Jam Bowl for Soup]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Adding Water Rice Pulping Machine/Soybean Grinding Machine]

Whitaker,J.R.(1963),High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame. Analytical Chemistry35(12), 1950-1953[Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Polypropylene Continuous Fiberglass Reinforced Thermoplastic CompositesAuthor for correspondence