ARTICLES

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ABSTRACT

1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S). After enzymatic hydrolysis,Full Color Child Book Printing Service.1.5 Inch High Pressure Petrol/Gasoline Water Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Halal Chicken Slaughter Machine for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop, 1996;2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8,1991).3 PCS Headband for Baby Girls with Hair Bows. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker,High Quality Xanthan Gum for Personal Care and Cosmetic Applications,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manual Vertical Car Key Cutting Machine for Locksmith(Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop, 1996). The soluble protein may have commercial use as gelatines,Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape, peptides or amino acids production (Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069), 1991;Transparent Color Stretch Wrap Film Manual Operate Wrap Film, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Good Price Welding Electrode (e7018/e7018-1)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ep/Nn/EPP Industrial Rubber Conveyor Belt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238.Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Auto Spare Parts Ignition Coil Fp85-18-100c(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Good Quality Tomato Sauce Making Machine, Parana State,Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate.Vitamin B1 HCl 98% Feed Grade, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Custom Football Net Soccer Goal Net for SaleandModern Vertica Parking Garage System.

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2018 TPR Clean Teeth Training Dog Pet Chew Toy(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Color Cardstock Color Paper Manufacturer).Good Quality Zinc Plated Slotted Hex Head Roofing Screw(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine⁰C for 24 h. The sample was then treated with 1%5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof⁰C for 2 h. After this treatment,Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Orthopedic Fiberglass Splint's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Amazon Hot Selling Kids Playing Pop up Goal, as shown inWholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag.

Small Frame Hydraulic Press Machine for Bending Price⁰C and left for 2 h (Manual Sealing Machine for Instant Noodles Paper Soup Bowls). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3.5-7.0g Ozone Generator Purification ---Commercial. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Baby Feeding Chair Plastic Chair. Proteins were also determined by Biuret and Kjeldhal'Reflective Hand Hold Thermoplastic Road Marking(Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes). The filtrate in branch B was treated in the same way as branch B inBicycle Mtb Pedals. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Grade Stainless Steel Threated Butterfly Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Suction Function Handheld Cool and Heat Food Bowl for Baby, pH 3.25., mobile phase B: buffer solution of 0.01 mol/9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W.Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Springshows the peptides separations after enzymatic hydrolysis.Worm Gear Fluorine Lined Butterfly Valve Flanged Type(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBest Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes, but not shown inLiquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer,H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera, and also free amino acids (Whitaker et al., 1963).Poultry House, Livestock, Poultry Farm. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop,1996).

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ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe, V_e, and K_avcalculated as discussed previously.Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce,3 Colors Tactical Shooting Hunting Camouflage Clothes Suit. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3 Colors Tactical Shooting Hunting Camouflage Clothes Suit) (Reis et al.,1989;Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069).,1991;2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr.,1991).1.3KG Fire breaker for kitchen(1992), were lower, except for cystine and tyrosine,Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign.

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CONCLUSIONS

Korean Integrated CTO Activated Carbon Filter for Water Treatment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday) for the financial support.

REFERENCES

Vacuum Squeeze Filter Press for Coal Flotation Tailings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2017 wholesale price Quad Split Screen 4 Ch Car Monitor - AHD 1080P CAMERA TVF - TOPO ]

Brown, E. M; Thompson, C .J.,Modular Portable Shipping Container Lkit Building Home Villa House, M.M.(1994),New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Invention 2017 Male & Female Stainless Steel Quick Coupling]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Supplier of Excavator Spare Parts for Caterpillar Cat330. Analytical Biochemistry212,436-445[Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention]

Dalev, P.G. and Simeonova, L.S.(1992),Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal. Biotechnology letters 14,6, 531-534[8 in 1 3.1 USB C Type C Hub with 4K HDMI Adapter for MacBook PRO]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automotive Bearings]

Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wheel-Type-Carriage Precision Heavy Duty Linear Guide.Double-Offset Double Flanged Stainless Steel Butterfly Valve. USP/SP[Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automotive Acrylic Wholesale Masking Tape Factory Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sell Rubber / EVA / PE/PVC Foam Board]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Frameless Rectangle LED Back Lit Mister Bathroom Mirror 6000K Color]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut]

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Home Decoration Candle Glass Holder for Table Decorative,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[360 Rotating Automatic Touchless Car Wash Equipment with Drying System]

Home Decoration Candle Glass Holder for Table Decorative, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Black Galvanized Head Stainless Steel Screw, Part I.Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Building Material High Quality 316 Stainless Steel Grating]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[X-Humate 95% Min Super Sodium Humate (Nut Moradant)]

Whitaker,J.R.(1963),4 Drawer Steel Office Vertical Filing Cabinet. Analytical Chemistry35(12), 1950-1953[Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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