ARTICLES

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ABSTRACT

Plastic Food Box Container Covers by Injection Mould Mold Tool. After enzymatic hydrolysis,304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment.Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Passenger Home Sightseeing Elevator Good Sales with Professional Technology;Vacuum Stainless Steel Casting Products; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Chicken Farming Materials Poultry Battery Cages Layer Battery Cage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, 1996;I-Tech 12000HD 2u Space Digital Professional Power Amplifier., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer,1991).High Quality Silicone Reusable Food Storage Bag China Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Polyurethane Gasket Foam Seal Dispensing Machine,Imee Print Customized Printing Agenda Printing Nb21,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml(1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, 1996). The soluble protein may have commercial use as gelatines,High Performance Multifunctional Crawler Mini Skid Steer Loader, peptides or amino acids production (Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, 1991;Chunke Portable UV Water Sterilizer for Water Purifier 150t, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Xjm Series Rubber Extruding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stop Dog From Jumping or Climbing Your Chain Link Fence..SGS Direct Thermal Label for Supermarket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Water Filter Polyester Non-Woven Fabric(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine⁰C and left for 2 h (Galfan Wire (Aluminium-Zinc Alloy coated)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch. Proteins were also determined by Biuret and Kjeldhal'Custom Stainless Steel Jewelry Production by Metal Injection Molding Process(LED Screen Display Wireless Charging 10000mAh Power Bank). The filtrate in branch B was treated in the same way as branch B inm.wanxuanglass.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Directly Selling Engine Standard Spark Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Logo Silicone Wristband, Rubber Wrist Bands. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom All Kinds of Acrylic Shoe Display Racks(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Medical Supply U40 Disposable Insulin Syringe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Magnetic Mixer Mixing Tank with Bottom Agitator (MTK). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Heavy Duty Marquee Advertising Pop up Tent) for the financial support.

REFERENCES

Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[up to 50m with IR]

Brown, E. M; Thompson, C .J.,New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), M.M.(1994),China Supplier Building Construction Steel Frame (ZY431).The Journal of the American Leather Chemists Association (JALCA)89(7)[2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing. Analytical Biochemistry212,436-445[Temperature Sensor Customized Thermostat for Industrial Appliances]

Dalev, P.G. and Simeonova, L.S.(1992),Lab Equipment portable Fume Hood. Biotechnology letters 14,6, 531-534[Silicone Large Non-Stick BPA Free Baking Mat Sheet]

Heidemann, E.(1997),Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt.Leder 48,(5),99-104[Articulated Forklift India]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale High Pressure 80L Nitrogen Gas Cylinder]

Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),10ton Telescopic Hydraulic Truck Crane.Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV. USP/SP[High Quality Cheap Price Custom Aluminum Logo Metal Label Plate]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Efficiency Mini-Pleat HEPA Filter (H13/H14)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Medical Supply U40 Disposable Insulin Syringe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Artificial Waterfall Wall]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manhole Galvanized Steel Trench Cover for Drain]

Cracker Multi Rows Trayless Packaging Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[German Technology Automatic Stacking System in Red Brick Automatic Production Line]

Cracker Multi Rows Trayless Packaging Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml]

Cracker Multi Rows Trayless Packaging Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2 Quick Change Flexible Roloc Disc, Part I.UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine]

High Quality Aluminium Windows Thermal Break China Supplier, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines.2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01, 175-184[700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board]

Whitaker,J.R.(1963),PRO-Installer Fiber Optic Termination Kit Lk-6016. Analytical Chemistry35(12), 1950-1953[Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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