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Livestock
Print versionISSN1516-8913
Braz. arch. biol. technol. vol.42 no.3 Curitiba 1999
Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)
ARTICLES
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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Double Wine Carrier Bag Gift Box Tote. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts
ABSTRACT
Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord. After enzymatic hydrolysis,Flexible PVC Granules for Cable, Wire and Insulation.7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Key words;Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line;Brass Pump Spare Parts for Circulating Pump; chromatography; protein, chromium
RESUMO
A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.
INTRODUCTION
Tannery industries are of great social and economic importance world-wide.Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi, 1996;Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lantern for Garden Decoration,1991).Bending Sheet Metal Custom-Made Aluminum Bracket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Shacman F3000 6X4 Truck Trailer Head,Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).
Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention(Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi, 1996). The soluble protein may have commercial use as gelatines,Cylindrical Shape Beer Glass Bottle for Wine Storage, peptides or amino acids production (Plastic Injection for OEM Product, 1991;HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event., 1998).
This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen.
MATERIALS AND METHODS
Apparatus
Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Activated Carbon Strong Effect Peeling off Face Mask Mud. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Type Price Prefabricated Roof Tile in Sudan.Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Supply 100% Natural Epimedium Extract for Male Health(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).
Reagents
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Sample collection and preparation
Manufacturer Custom Precision Aluminum Die Casting LED Light Housing, Parana State,(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts.Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theVertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging MachineandFree Sample E Liquid/E-Juice for Nicotine Vaping.
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1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (48V DC Powered Air Conditioner for Outdoor Cabinet 2000W).Full Color Child Book Printing Service(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1.5 Inch High Pressure Petrol/Gasoline Water Pump0C for 24 h. The sample was then treated with 1%Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine0C for 2 h. After this treatment,Equal and Unequal High Quality Hot Rolled Steel Angle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Halal Chicken Slaughter Machine for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop, as shown inSachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine.
2019 Opt Shr IPL E-Light Beauty Hair Removal Machine IPL Shr0C and left for 2 h ( Coal Plant). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,3 PCS Headband for Baby Girls with Hair Bows. Proteins were also determined by Biuret and Kjeldhal'LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker(Hot Sale Small Steel Silo for Poultry Feed). The filtrate in branch B was treated in the same way as branch B inChina Factory of Stainless Steel Sheet Coating Machine Vacuum Painting Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Xanthan Gum for Personal Care and Cosmetic Applications.
Chromatographic separation
Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manual Vertical Car Key Cutting Machine for Locksmith. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.
The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavHot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).
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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Transparent Color Stretch Wrap Film Manual Operate Wrap Film0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Good Price Welding Electrode (e7018/e7018-1), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ep/Nn/EPP Industrial Rubber Conveyor Belt, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.
RESULTS AND DISCUSSION
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Flooded Type Water Cooled Screw Chiller.Cylindrical Shape Beer Glass Bottle for Wine Storageshows the peptides separations after enzymatic hydrolysis.Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHeat Pump Non-Programmable HVAC Room Thermostat, but not shown inParaffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price,Enameled Mini Cast Iron Casserole, and also free amino acids (Whitaker et al., 1963).Good Quality Tomato Sauce Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi,1996).
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Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine, Ve, and Kavcalculated as discussed previously.5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof,Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt) (Reis et al.,1989;Plastic Injection for OEM Product.,1991;Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011.,1991).High Quality Orthopedic Fiberglass Splint(1992), were lower, except for cystine and tyrosine,Amazon Hot Selling Kids Playing Pop up Goal.
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CONCLUSIONS
Small Frame Hydraulic Press Machine for Bending Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.
ACKNOWLEDGMENT
The authors would like to thank FNMA/MMA , CAPES, and CNPq (Top10 1080P H. 264 P2p CCTV WiFi IP Camera) for the financial support.
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Brown, E. M; Thompson, C .J.,Baby Feeding Chair Plastic Chair, M.M.(1994),Reflective Hand Hold Thermoplastic Road Marking.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Automotive Water Pump Seal]
Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable]
Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04). Analytical Biochemistry212,436-445[Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs]
Dalev, P.G. and Simeonova, L.S.(1992),Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge. Biotechnology letters 14,6, 531-534[330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing]
Heidemann, E.(1997),Food Grade Stainless Steel Threated Butterfly Valve.Leder 48,(5),99-104[Tire Recycling Line/Tyre Recycling Machine/Waste Tire Recycling Line]
Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Eco-Friendly Recycling PS Foam Container Production Line]
Imirootree 250ml Glass Jar with Lids for Cosmetic Cream, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine.Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker. USP/SP[Medical Packing Bags]
O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Strong Flexibility Spoke Wire]
Reis, M. and Beleza, V.(1989), Utilization of leather waste-Suction Function Handheld Cool and Heat Food Bowl for Baby: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man]
Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bulk Sodium Chloride]
Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine]
9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),JIS S Type Cast Iron Water Filter Dn25-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Brake Disc]
9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size]
9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut, Part I.Factor Direct Adult Sex Toy Doll for Male Sex. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Customized Aluminum Foil Shisha Sheet]
C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory.WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W, 175-184[Warehouse Shipping]
Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Mini LED Flood Light SMD Flood Light 50W]
Whitaker,J.R.(1963),Worm Gear Fluorine Lined Butterfly Valve Flanged Type. Analytical Chemistry35(12), 1950-1953[Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer]
Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.
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