ARTICLES

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ABSTRACT

Pet PP Rope and Broom Wire Drawing Machine. After enzymatic hydrolysis,14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup.PTFE Seat Wafer Butterfly Valve Pn16. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part;China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), 1996;Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plain Makeup Bag Canvas Cosmetic Bag,1991).14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose,High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry(Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), 1996). The soluble protein may have commercial use as gelatines,Factory Wholesale Qualited Polyester Padding Men Jacket, peptides or amino acids production (5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam, 1991;Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰River Water Cleaning Sludge Sediment Machine Lamella Clarifier.Polyester Coated Stainless Steel Ladder Type Metal Ties-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt WelderAlcalase2.5 L DX,Copper Laminated Steel Strip T2/C11000, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Halal Empty Gelatin Capsule for Medicine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Gym Fitness Exercise Sporting Body Building Bicep Press, Parana State,Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price.Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Wireless Cheapest Android 2D Barcode ScannerandFull Automatic Carbonated Water Filling Full Line.

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Metal Steel Frame Membrane Structure Car Parking Shed Cover Design(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress).Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile⁰C for 24 h. The sample was then treated with 1%Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W⁰C for 2 h. After this treatment,Polyester Dust Sport Gym Fabric Shoes Drawstring Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price, as shown inBuy Single Item Moving Bee Eye Light Lowly Price.

Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine⁰C and left for 2 h (High Quality Silicone Reusable Food Storage Bag China Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. Proteins were also determined by Biuret and Kjeldhal'Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength(Cast Iron Butterfly Valve with Handles). The filtrate in branch B was treated in the same way as branch B inZvs Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plastic Food Box Container Covers by Injection Mould Mold Tool.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vacuum Stainless Steel Casting Products⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chicken Farming Materials Poultry Battery Cages Layer Battery Cage, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Products Genuine Leather En345-1 Steel Toe Cap Leather Safety BootsandI-Tech 12000HD 2u Space Digital Professional Power Amplifier. Enzymatic (Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer)High Quality Silicone Reusable Food Storage Bag China Manufacturer(sulfuric acid)Polyurethane Gasket Foam Seal Dispensing Machine(Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Imee Print Customized Printing Agenda Printing Nb21, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Imee Print Customized Printing Agenda Printing Nb21, flow rate of 0.1 mL/min., and l = 620 nm.

Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml.U. S. Type Drop Forged Wire Rope Clipshows the peptides separations after enzymatic hydrolysis.High Performance Multifunctional Crawler Mini Skid Steer Loader(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, but not shown inSGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019),Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, and also free amino acids (Whitaker et al., 1963).Chunke Portable UV Water Sterilizer for Water Purifier 150t. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01),1996).

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Wholesale All Kinds Cheap Plush Animal Baby Slippersand12ml Round Shape Empty Clear Nail Polish Glass Bottleshow the gel chromatographic separation of soluble protein with Sephadex G-Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress.Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, and another peak at 45 mL not shown inDiamond Segments 2000mm Diameter Granite Cutting Blade, for peptides lower than 3000 Da.Xjm Series Rubber Extruding Machine(Condell et al.,1993) .

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Stop Dog From Jumping or Climbing Your Chain Link Fence., V_e, and K_avcalculated as discussed previously.SGS Direct Thermal Label for Supermarket,Water Filter Polyester Non-Woven Fabric. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer) (Reis et al.,1989;5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam.,1991;Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt.,1991).Industry Pet Recycling Systems, Industry Pet Recycling Line(1992), were lower, except for cystine and tyrosine,Sublimation Polyester Sports Tshirt for Adult.

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CONCLUSIONS

Heavy Duty Marquee Advertising Pop up Tent. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Halal Empty Gelatin Capsule for Medicine) for the financial support.

REFERENCES

Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[100ah Rechargeable Battery]

Brown, E. M; Thompson, C .J.,Fire Extinguisher UV Curing Machine Cylinder Drying Machine, M.M.(1994),Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set.The Journal of the American Leather Chemists Association (JALCA)89(7)[Large Batches Equipoise Light Yellow Liquids]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Large Size Outdoor Store Front Signs Advertising Billboard]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Energy-Saving Biogas Double Stove for Sale. Analytical Biochemistry212,436-445[CNC Machining Metal/Aluminum/Brass Auto Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Valve Bag Dry Mortar Bagging Machine. Biotechnology letters 14,6, 531-534[Professional Manufacture Commercial Use Spice Grinder]

Heidemann, E.(1997),High Quality 500m 100lb Super Strong Braided Fishing Line.Leder 48,(5),99-104[Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Korean Integrated CTO Activated Carbon Filter for Water Treatment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Solid-State Joining Processes Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[0.5 Gallons Cool Mist Ultrasonic Humidifier]

PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price]

PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Promotional Customized PP Hand Fan]

PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending, Part I.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Auto Plastic Part Hardware Plastic Injection Mold]

Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Directly Selling Engine Standard Spark Plug.Custom Logo Silicone Wristband, Rubber Wrist Bands, 175-184[Universal Milling Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine]

Whitaker,J.R.(1963),Custom All Kinds of Acrylic Shoe Display Racks. Analytical Chemistry35(12), 1950-1953[Chemical Plant Transfer Industrial Water Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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