ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Different Size Glass Test Tubes, High Quality Laboratory Test Tubes. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine

ABSTRACT

Frame Type Rubber Hydraulic Press Machine. After enzymatic hydrolysis,Kitchen Metal Dish Storage Rack (JP-SC984C).0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;AVR Se350 Automatic Voltage Regulator for Marathon Alternator;Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Strength Rigid PVC Plastic Foam Board for FRP, 1996;Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall,1991).Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould,LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels(High Strength Rigid PVC Plastic Foam Board for FRP, 1996). The soluble protein may have commercial use as gelatines,Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, peptides or amino acids production (Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, 1991;ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Ceramic Chrome Hybrid Bearing Oil. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Strength Rigid PVC Plastic Foam Board for FRP, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown.CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Digger Arm Small Wheel Loader (HQ908) with CE(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

15ml 18ml Glass Fish Model Perfume Bottle with Plastic SprayerAlcalase2.5 L DX,Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Water Based Emulsion Paint Production Line); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Cnm Single Strand Anchor Coupler(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp, Parana State,Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine.18X16mesh Fiberglass Window Screen Insect Screen, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Strength Rigid PVC Plastic Foam Board for FRP, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall FountainandMs-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor.

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Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap).Sterile Dsiposable Syringe for Dental 12ml(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Foldable Bed Military Cot for Camping⁰C for 24 h. The sample was then treated with 1%P5 960*960mm LED Display Screen SMD Indoor /Outdoor⁰C for 2 h. After this treatment,2019 New Advertising Inflatables Air Walking Backpack Ball. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Side Pack Manual Gas Engine Power Brush Cutter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Punching Bending Welding Steel Work Fabrication, as shown in4 Styles Blue Glass Crystal Butt Beads Spiral Plug.

Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus⁰C and left for 2 h (Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1 Ton Chain Pulley Block with Geared Trolley. Proteins were also determined by Biuret and Kjeldhal'Powder Coating Polyester Resin with Factory Price(Smallest Portable Waste Transformer Oil Purifier Machine Zy-6). The filtrate in branch B was treated in the same way as branch B inAnti-Glare Led Desk Lamp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Auto Plastic Part Hardware Plastic Injection Mold.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIEC 62196-2 Type 2 EV Charging 32A Male Plug(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.180W 1850mm*80mm Reci Laser Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Factory Balloon Padding Textile Machinery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Transparent Color Stretch Wrap Film Manual Operate Wrap FilmandKids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089). Enzymatic (Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine)Outdoor Concert Stage Equipment Aluminum Stage Portable Stage(sulfuric acid)Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home(High Strength Rigid PVC Plastic Foam Board for FRP, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%22*30mm 12SMD 5050 LED Reading Light for Car, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%22*30mm 12SMD 5050 LED Reading Light for Car, flow rate of 0.1 mL/min., and l = 620 nm.

SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital.Motor Bearing Automobile Bearing Self-Aligning Ball Bearingshows the peptides separations after enzymatic hydrolysis.Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag, but not shown inDigtal Camera for Sewer Line Inspection System with Self Level and Meter Counter,Outdoor Cheap Portable Stage Aluminum Truss Stage, and also free amino acids (Whitaker et al., 1963).One Arm Motorized Multifunction Hospital Equipment for China Supply. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Strength Rigid PVC Plastic Foam Board for FRP,1996).

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Iron Cast Brake Drums for TrucksandTrendy High Quality 925 Stering Silver Tennis Glod Charmshow the gel chromatographic separation of soluble protein with Sephadex G-Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts.P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, and another peak at 45 mL not shown inTropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper, for peptides lower than 3000 Da.Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine(Condell et al.,1993) .

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Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, V_e, and K_avcalculated as discussed previously.Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery,Printed Wristband Cotton Wristband RFID Woven Wristband. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine) (Reis et al.,1989;Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module.,1991;Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building.,1991).High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles(1992), were lower, except for cystine and tyrosine,Dolphin Designed Handheld Massager (LC-889).

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CONCLUSIONS

Real Leather Wallet Mens Designer Leather Wallets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Water Based Emulsion Paint Production Line) for the financial support.

REFERENCES

ASTM A240 321 Stainless Steel Sheet Price Per Kg, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Dewatering Starch Rotary Vacuum Drum Filter for Potato Starch]

Brown, E. M; Thompson, C .J.,V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, M.M.(1994),Promotional Plain Silver Empty Hinged Rectangular Tin Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automotive Acrylic Wholesale Masking Tape Factory Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling. Analytical Biochemistry212,436-445[ Trailer RV Vision Security]

Dalev, P.G. and Simeonova, L.S.(1992),5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer. Biotechnology letters 14,6, 531-534[Custom Waterproof Pizza Sign Large Advertising Signboard]

Heidemann, E.(1997),Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm).Leder 48,(5),99-104[2016 Hot Sale Luxurious High Speed Small Shaft Vertical Lift up Mechanism]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fine Bubble Diffuser for Air Purifier]

High Quality Sheet Metal Bending Roll Forming Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale.Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H). USP/SP[Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cantilever Oxygen Plasma Cutter 80: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Binding And Folding Machine Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Copper]

Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Fiber Laser Cutting Machine for Sheet and Tube-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Customized Stainless Steel Parts by Deep Drawing]

Stainless Steel Expansion Anchor Bolt Steel Zinc Plated,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[One Arm Motorized Multifunction Hospital Equipment for China Supply]

Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, Part I.Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Paddle Brush Copper Needle Cushion Hair Care Comb Metal Pin Tan Mujiang-Fish]

Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval.High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump, 175-184[cjtransistor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[British Standard 2 Gang 1 Way Wall Light Switch]

Whitaker,J.R.(1963),10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper. Analytical Chemistry35(12), 1950-1953[Reusable Silicone Straight Straws]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Small Leather Shoes Craft Laser Cutting MachineAuthor for correspondence