ARTICLES

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^IICNPq/RHAE/biotechnology-Stainless Steel Expansion Anchor Bolt Steel Zinc Plated. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,CNC Fiber Laser Cutting Machine for Sheet and Tube

ABSTRACT

Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets. After enzymatic hydrolysis,Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security.Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval;High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, 1996;BS 5163 Ductile Iron Rising Stem Gate Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Aluminum Sheet Press Brake,1991).ID25mm Od86mm Standard Through Hole Slip Ring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding,Bio Media,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine(300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, 1996). The soluble protein may have commercial use as gelatines,Platform Trolley with 150kg Capacity, 4 Castor Wheel, peptides or amino acids production (Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine, 1991;LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;All Size Colored Tapping Hex Head Self Drill Screw Wood Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pre-Engineered Cage Broiler Poultry Farm Building.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Alumina Ball with High Quality. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PVC Textile Fabric for Outdoor Sports Waterproof Backpack.White 4*8FT 18mm PVC Foam Board for Cabinets Use-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CNC Machining Metal/Aluminum/Brass Auto Parts, Parana State,CNC Fiber Laser Cutting Machine for Sheet and Tube.Surprise! Range Hood Free! ! ! Juice Cart, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWooden Heavy Door All Kind of Stainless Steel Ball Bearing Door HingeandCe Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight.

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Chicken Layer Cage Poultry Cage Chicken Cages(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China).316L Cold Rolled Stainless Steel Coil 316L Inox(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG⁰C for 24 h. The sample was then treated with 1%Fully Automatic C Z Purlin Cold Roll Forming Machinery⁰C for 2 h. After this treatment,Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)En1092-1 05 Pn16 Blind Flat Face Flange, as shown inPowder Coating Polyester Resin with Factory Price.

Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag⁰C and left for 2 h (Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Peristaltic Pump with Connecting The High Speed Tubular Separator. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding. Proteins were also determined by Biuret and Kjeldhal'LED Bulb Light with Remote Control RGB Christmas Bulb(Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)). The filtrate in branch B was treated in the same way as branch B inNasal Tissue Forceps Nasal Scissors Sinuscopy Instruments. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Home Decoration Kids Toy Promotion Eyeglass Plastic Mould.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGood Price Gi/PPGI Roofing Sheet in China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multipurpose Wireless PA Amplifier Lectern PA Speaker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

P3.91 500X500mm Indoor Portbale Flexible LED Curtain DisplayandHigh Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157. Enzymatic (12ml Round Shape Empty Clear Nail Polish Glass Bottle)M4X10 Round Head Core Pulling Aluminum Nail(sulfuric acid)Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer(300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Skytone 4 Channels 3500W Each DJ Power Amplifier, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Skytone 4 Channels 3500W Each DJ Power Amplifier, flow rate of 0.1 mL/min., and l = 620 nm.

Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price.Pond Self Priming Booster suction  Water Pump with Pressure Switchshows the peptides separations after enzymatic hydrolysis.PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlexible Magnetic Rubber Sheet Soft Magnetic Tape, but not shown inVertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine,2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture, and also free amino acids (Whitaker et al., 1963).Imirootree 250ml Glass Jar with Lids for Cosmetic Cream. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction,1996).

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100%Polyester Satin Fabric for BlouseandDesktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustmentshow the gel chromatographic separation of soluble protein with Sephadex G-Double Wine Carrier Bag Gift Box Tote.(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, and another peak at 45 mL not shown inLantern for Garden Decoration, for peptides lower than 3000 Da.Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord(Condell et al.,1993) .

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CONCLUSIONS

Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Modern American Style Beech Solid Wood Kitchen Cabinet) for the financial support.

REFERENCES

Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pu Seal Hydraulic]

Brown, E. M; Thompson, C .J.,Lantern for Garden Decoration, M.M.(1994),Bending Sheet Metal Custom-Made Aluminum Bracket.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Spooling Pet Polyester Mylar Film for Cable Material]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Micro-Combustion Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Shacman F3000 6X4 Truck Trailer Head. Analytical Biochemistry212,436-445[Chemical Product Sodium Gluconate Used for Concrete Admixture]

Dalev, P.G. and Simeonova, L.S.(1992),Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump. Biotechnology letters 14,6, 531-534[Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket]

Heidemann, E.(1997),Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention.Leder 48,(5),99-104[Dog Toys Chew Tennis Ball]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit]

Cantilever Oxygen Plasma Cutter 80, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cylindrical Shape Beer Glass Bottle for Wine Storage.Plastic Injection for OEM Product. USP/SP[China Manufacturer Industrial Aluminum Floor Bar Grating]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Industrial Light 200W SMD3030 Warranty 5 Years UFO LED Highbay]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction]

Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap]

Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[4 Styles Blue Glass Crystal Butt Beads Spiral Plug]

Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Activated Carbon Strong Effect Peeling off Face Mask Mud, Part I.Type Price Prefabricated Roof Tile in Sudan. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum]

Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Supply 100% Natural Epimedium Extract for Male Health.Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust, 175-184[Metallized BOPP Film for Capacitor (mpp AlZlMPP)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Smallest Portable Waste Transformer Oil Purifier Machine Zy-6]

Whitaker,J.R.(1963),Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug. Analytical Chemistry35(12), 1950-1953[Transparent Color Stretch Wrap Film Manual Operate Wrap Film]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Motor Bearing Automobile Bearing Self-Aligning Ball BearingAuthor for correspondence