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ABSTRACT

Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. After enzymatic hydrolysis,Stainless Steel Ozone Disinfection Machine Ozone Generator.Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PVC Profile with Net Making Machine Used for Window Sealing Profile;Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bobby Flay Websites Restaurant Martha Stewart Designer Tray, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, 1996;Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Diameter 2.68m Paragraphed Autoclave for AAC Bricks,1991).Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Travelling Weekender Mens Canvas Duffle Gym Sports Bag,4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)(Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, 1996). The soluble protein may have commercial use as gelatines,Best Selling Household Living Room Mat Chenille Carpets, peptides or amino acids production (130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, 1991;Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Large Size Outdoor Store Front Signs Advertising Billboard., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1325 CNC Engraving Router. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel.T6s 86 PCD Core Bit for Geotechnical Drilling-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

NPK 15 15 15 Compound Fertilizer at a Hot Selling, Parana State,Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration.Mini Blown Film Extrusion, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTaiding Custom Tank Sheet Metal FabricationandBS 5163 Ductile Iron Rising Stem Gate Valve.

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Pearls Flake 99% Factory Naoh Caustic Soda(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286).Auto Spare Parts 12629037 Ignition Coil for Cadillac(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality PVC Resin with Low Price⁰C for 24 h. The sample was then treated with 1%Small Backhoe Loader with Air Conditioning⁰C for 2 h. After this treatment,Line Scan Uvss System Portable Under Vehicle Surveillance. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)FSTN Type Monochrome Graphic Display Cog LCD with Backlight, as shown inIndustrial Grade General Purpose Titanium Dioxide Rutile/Anatase.

3D Embossed Iron on Label Clothing Heat Transfer Label⁰C and left for 2 h (4-14 Manual Staple Gun with Staples for Hand Tools). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Glass Food Jar & Plastic Lid. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer. Proteins were also determined by Biuret and Kjeldhal'Easy Operation Low Investment Cassava Silo(Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile). The filtrate in branch B was treated in the same way as branch B inOsb Manufacturers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBuilding Material High Quality 316 Stainless Steel Grating(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sc-2400 Automatic Multi Function Sosn Side Drilling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Quality Shaper Machine Bc6085 Shapping Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Flower Design Custom Made Silk Scarf, V_e, and K_avcalculated as discussed previously.New Design Indoor Artificial Mini Rockery Fountains,7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Anti Slip Rubber Mat) (Reis et al.,1989;130HP 4WD Large Farm Power Engine Tractor From High Quality Factory.,1991;Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber.,1991).Medical Endoscope Disposable Bite Block with Bandage(1992), were lower, except for cystine and tyrosine,Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels.

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CONCLUSIONS

Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Round Shape All Color Pets Feeding Dog Bowl of China Supplies) for the financial support.

REFERENCES

Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sc 1412 Shock Absorbers]

Brown, E. M; Thompson, C .J.,12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, M.M.(1994),Fine Bubble Diffuser for Air Purifier.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore. Analytical Biochemistry212,436-445[Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. Biotechnology letters 14,6, 531-534[Prefab Building Insulated Interior Concrete Partition Walls]

Heidemann, E.(1997),3120/17/38 Boltable Crane Rail Clips.Leder 48,(5),99-104[Sand Drum Washer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ASTM Iron Sand Casting Parts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Auto Spare Parts Car Auto Water Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device]

Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop]

Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Jacquard Football Fans Acrylic Sports Warmer Scarf]

Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag, Part I.Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry]

High Quality & High Speed Straight Line Wire Drawing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Speed 3000W Fiber Laser Cutter.Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, 175-184[Hot sale mild flat steel with best price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Crop Fertilizer Agriculture Nh4cl Ammonium Chloride]

Whitaker,J.R.(1963),3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting. Analytical Chemistry35(12), 1950-1953[10ml Dropper Bottle for Skin Care Packing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for AdultsAuthor for correspondence