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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Tumble Belt Type Shot Blasting Machine for Iron Castings Parts

ABSTRACT

Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple. After enzymatic hydrolysis,Good Quality Good Price China Plastic Injection Molding Machine.Pet Hair Remover Glove for Dog, Grooming Gloves Brush. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa;Alumina Balls for Hydogen Peroxide; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Anti-High Pressure Insulated Pliers with PVC Dipped Handles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Selling Electronical Blue Pet Plastic Release Film Price, 1996;DIN127 Stainless Steel Spring Lock Washer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom,1991).New Condition Hot Sale Automatic Animal Feed Cooler Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System,Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Garlic Red Mesh Bag(Hot Selling Electronical Blue Pet Plastic Release Film Price, 1996). The soluble protein may have commercial use as gelatines,Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder, peptides or amino acids production (Ground Finish Molybdenum Tzm Rod, 1991;Transparent/Clear BOPP Jumbo Roll, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Yashi 19 270degree Pyramid Hologram Display Showcase Attractive., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Chenille Microfiber Mop Head.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Waterproof Pizza Sign Large Advertising Signboard. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Selling Electronical Blue Pet Plastic Release Film Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PVC Shrink Sleeve Label for Drinking Water Bottle.Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Galvanized Square Wire Mesh, Metal Window Screen(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cherry Wooden Grain PVC Film in Furniture(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam).Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV⁰C for 24 h. The sample was then treated with 1%Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)⁰C for 2 h. After this treatment,Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Linen Quiltcover Set with Quilting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, as shown in Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building.

Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace⁰C and left for 2 h (Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Teerte Square Flat Head Self Tapping Screw. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard. Proteins were also determined by Biuret and Kjeldhal'Bto 22 Electric Galvanized Razor Concertina Wire for Military(20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China). The filtrate in branch B was treated in the same way as branch B inCase Erector and Bottom Sealer Carton Erecting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Selling Military Large Event Marquee Tent Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Marine High Visibility Solas Reflective Tape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hemp Seed Oil Body Lotion Moisturizes The Skinand24L Thermoelectric Cooler and Warmer Car Mini Car Fridge. Enzymatic (Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444))Top Quality Waterproof IP66 Aluminum Die Cast Junction Box(sulfuric acid)Magic Tape Hook and Loop Fasteners(Hot Selling Electronical Blue Pet Plastic Release Film Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, flow rate of 0.1 mL/min., and l = 620 nm.

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BS1387 Class B 2 Inch Sch40 Gi Pipe GalvanizedandShima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Conditionshow the gel chromatographic separation of soluble protein with Sephadex G-Fashion Soft PVC Rubber Keychains for Promation Gift.Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper, and another peak at 45 mL not shown in LED Bayomet Vehicle Lamps, for peptides lower than 3000 Da.Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price(Condell et al.,1993) .

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Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes, V_e, and K_avcalculated as discussed previously.Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments,Factory Supply Cheap Custom Bleached White Cotton Gloves. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic CNC Glass Cutting Machine Glass Cutting Table) (Reis et al.,1989;Ground Finish Molybdenum Tzm Rod.,1991;DIN127 Stainless Steel Spring Lock Washer.,1991).2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle(1992), were lower, except for cystine and tyrosine,304 &314 Stainless Steel Seamless Bending Tube.

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CONCLUSIONS

Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Customized Stainless Steel Parts by Deep Drawing) for the financial support.

REFERENCES

Custom Grey Full-Zip Cotton Mens Hoodies, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fiberglass Insulation Material Epoxy G10/Fr4 Sheet]

Brown, E. M; Thompson, C .J.,Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook, M.M.(1994),7inch Industrial Tablet Computer Naked Resistance Touch Screen.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[18X16mesh Fiberglass Window Screen Insect Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Glass Door Passenger Sightseeing Elevator for Sale. Analytical Biochemistry212,436-445[Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap]

Dalev, P.G. and Simeonova, L.S.(1992),Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys. Biotechnology letters 14,6, 531-534[FRP Counter Flow Low Noise Round Type Cooling Tower 600rt]

Heidemann, E.(1997),12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor.Leder 48,(5),99-104[Cnc Machine Programming]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pipe Conveyor Belt]

Acm Dewatering Twin Screw Extruder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple.Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase. USP/SP[Hydraulic Orbital Valve Power Steering]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fcy30 Transport Mini Tipping Dumper: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Large Ss Stamping/ Bending Sheet Metal Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[160-450mm HDPE PE Irrigation Gas Pipe Making Machine Extrusion Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hanging Necklace Fan USB Rechargeable Battery Operated Fan]

Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chicken Farming Materials Poultry Battery Cages Layer Battery Cage]

Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OPP Printed Block Bottom Bags]

Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Premium Grade Rubber Cloth Duct Tape, Part I.Sud1000h Poly Welder Butt Fusion Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle]

Customized Anti-Rust Torsion Spring, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining.New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, 175-184[300Ton Welding Rotator Turning Roll]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump]

Whitaker,J.R.(1963),Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60. Analytical Chemistry35(12), 1950-1953[Hot Sale Electric LED light Aroma Diffuser]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective VestAuthor for correspondence