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ABSTRACT

Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch. After enzymatic hydrolysis,Bobby Flay Websites Restaurant Martha Stewart Designer Tray.Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber;Diameter 2.68m Paragraphed Autoclave for AAC Bricks; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Travelling Weekender Mens Canvas Duffle Gym Sports Bag, 1996;4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC),1991).Best Selling Household Living Room Mat Chenille Carpets. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,130HP 4WD Large Farm Power Engine Tractor From High Quality Factory,Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Large Size Outdoor Store Front Signs Advertising Billboard(Travelling Weekender Mens Canvas Duffle Gym Sports Bag, 1996). The soluble protein may have commercial use as gelatines,Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, peptides or amino acids production (1325 CNC Engraving Router, 1991;Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;T6s 86 PCD Core Bit for Geotechnical Drilling., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Machining 6601 Aluminum Parts with Anodized Automoblile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Travelling Weekender Mens Canvas Duffle Gym Sports Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cummins L10 Engine Piston Cooling Nozzle 3070218.Round Shape All Color Pets Feeding Dog Bowl of China Supplies-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wvrb Series Planetary Gearbox Used for Robot(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality PVC Resin with Low Price, Parana State,PVC Profile with Net Making Machine Used for Window Sealing Profile.Small Backhoe Loader with Air Conditioning, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Travelling Weekender Mens Canvas Duffle Gym Sports Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePVC Shrink Sleeve Label for Drinking Water Bottleand750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm.

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Line Scan Uvss System Portable Under Vehicle Surveillance(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)).Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%FSTN Type Monochrome Graphic Display Cog LCD with Backlight⁰C for 24 h. The sample was then treated with 1%3D Embossed Iron on Label Clothing Heat Transfer Label⁰C for 2 h. After this treatment,Glass Food Jar & Plastic Lid. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Easy Operation Low Investment Cassava Silo, as shown inStarry Laser Holographic Nail Glitter Powder Copper Shining Glitters.

CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making⁰C and left for 2 h (Hot Sale Biodegradable and Recycled Paper Straws). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Building Material High Quality 316 Stainless Steel Grating. Proteins were also determined by Biuret and Kjeldhal'Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table(Construction & Decoration ERW Square Steel Tube Manufacturer). The filtrate in branch B was treated in the same way as branch B inPortable Medical Electrical Equipment for Ozone Therapy. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sc-2400 Automatic Multi Function Sosn Side Drilling Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQuality Shaper Machine Bc6085 Shapping Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

M4X10 Round Head Core Pulling Aluminum NailandPolyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5. Enzymatic (Free Sample! AC Power Contactor for Sale)PA System Loudspeaker 100W Outdoor Column Speaker (sulfuric acid)Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel(Travelling Weekender Mens Canvas Duffle Gym Sports Bag, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Breathable Surgical Disposable 3 Ply Earloop Face Mask, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Breathable Surgical Disposable 3 Ply Earloop Face Mask, flow rate of 0.1 mL/min., and l = 620 nm.

Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9.EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Priceshows the peptides separations after enzymatic hydrolysis.Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Metal Window Screen, but not shown inHigh Quality Casting Cover Aluminum Die Casting Products,Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, and also free amino acids (Whitaker et al., 1963).Flower Design Custom Made Silk Scarf. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Travelling Weekender Mens Canvas Duffle Gym Sports Bag,1996).

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Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, V_e, and K_avcalculated as discussed previously.Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08),Ladder Type Cable Tray with Ce/ GOST/ TUV/UL. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine) (Reis et al.,1989;1325 CNC Engraving Router.,1991;4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box.,1991).12V Electric Portable Outdoor Water Fruit Car Fridge with Ce(1992), were lower, except for cystine and tyrosine,Fine Bubble Diffuser for Air Purifier.

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CONCLUSIONS

Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pearls Flake 99% Factory Naoh Caustic Soda) for the financial support.

REFERENCES

High Quality Cheap Price Custom Aluminum Logo Metal Label Plate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Display Panel Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,3120/17/38 Boltable Crane Rail Clips, M.M.(1994),2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide.The Journal of the American Leather Chemists Association (JALCA)89(7)[No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Factory Queen Mattress with Visco Memory Foam and Latex]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Supplier Rubber Injection Molding Machine with Ce. Analytical Biochemistry212,436-445[9~12 Inch Mechanical Backing Plate]

Dalev, P.G. and Simeonova, L.S.(1992),Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China. Biotechnology letters 14,6, 531-534[Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck]

Heidemann, E.(1997),Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery.Leder 48,(5),99-104[Order Make Anti Theft Auto Window Cable Electronic Wire Harness]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[7PCS RGBW LED Plastic PAR Light Wall Washer]

Stainless Steel Ozone Disinfection Machine Ozone Generator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free.Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag. USP/SP[Chicken Layer Cage Poultry Cage Chicken Cages]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ASA-UPVC Anti-Corrosive Composite Roof Tile]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coal Mining Steel Transition Idlers for Belt Conveyor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sleeved Wire Harness for Industry Equipment]

High Quality & High Speed Straight Line Wire Drawing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Speed 3000W Fiber Laser Cutter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Promotional Reusable Foldable PP Non Woven Shopping Bag]

High Quality & High Speed Straight Line Wire Drawing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PVC Cross Joint Pipe Fittings Equal Pipe Cross]

High Quality & High Speed Straight Line Wire Drawing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, Part I.3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries]

Personality Cat Eye Sunglasses, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline.UV Coated PVC Panel for Interior Wall Board, 175-184[China High Quality Satin Ribbon]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers]

Whitaker,J.R.(1963),Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.. Analytical Chemistry35(12), 1950-1953[8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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