ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pet PP Rope and Broom Wire Drawing Machine, 1996;14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PTFE Seat Wafer Butterfly Valve Pn16,1991).Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults,Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)(Pet PP Rope and Broom Wire Drawing Machine, 1996). The soluble protein may have commercial use as gelatines,Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt, peptides or amino acids production (Plain Makeup Bag Canvas Cosmetic Bag, 1991;14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pet PP Rope and Broom Wire Drawing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Wholesale Qualited Polyester Padding Men Jacket.5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Polyester Coated Stainless Steel Ladder Type Metal Ties, Parana State,Sanjing PVC Lining Layflat Fire Hose.2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pet PP Rope and Broom Wire Drawing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLm Series Miniature Lm8uu Liner Ball Bush Bushing Ball BearingandBaijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring.

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SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer).Copper Laminated Steel Strip T2/C11000(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Halal Empty Gelatin Capsule for Medicine⁰C for 24 h. The sample was then treated with 1%Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm⁰C for 2 h. After this treatment,Gym Fitness Exercise Sporting Body Building Bicep Press. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Steel Frame Membrane Structure Car Parking Shed Cover Design, as shown inCompetitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody.

Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size⁰C and left for 2 h (Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W. Proteins were also determined by Biuret and Kjeldhal'Polyester Dust Sport Gym Fabric Shoes Drawstring Bag(Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp). The filtrate in branch B was treated in the same way as branch B inDin3352 F4 Gate Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avServo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Food Box Container Covers by Injection Mould Mold Tool, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Xjm Series Rubber Extruding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat) for the financial support.

REFERENCES

Stop Dog From Jumping or Climbing Your Chain Link Fence., A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[HiFi Music TV]

Brown, E. M; Thompson, C .J.,SGS Direct Thermal Label for Supermarket, M.M.(1994),Water Filter Polyester Non-Woven Fabric.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sell Rubber / EVA / PE/PVC Foam Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industry Pet Recycling Systems, Industry Pet Recycling Line. Analytical Biochemistry212,436-445[Vertical Type Caseformer Machine WJ-LKX-15]

Dalev, P.G. and Simeonova, L.S.(1992),Sublimation Polyester Sports Tshirt for Adult. Biotechnology letters 14,6, 531-534[360 Rotating Automatic Touchless Car Wash Equipment with Drying System]

Heidemann, E.(1997),Heavy Duty Marquee Advertising Pop up Tent.Leder 48,(5),99-104[MYK1224 Two Axis CNC Surface Grinding Machine (Hydraulic CNC Grinder)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Building Material High Quality 316 Stainless Steel Grating]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PP Foam Stationary Sheet Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[All In One Pc Oem]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Downspout Pipe Roll Forming Machine]

Energy-Saving Biogas Double Stove for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Valve Bag Dry Mortar Bagging Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag]

Energy-Saving Biogas Double Stove for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus]

Energy-Saving Biogas Double Stove for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality 500m 100lb Super Strong Braided Fishing Line, Part I.20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew]

37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags.PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, 175-184[Electronic Survey Equipment Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent]

Whitaker,J.R.(1963),Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine. Analytical Chemistry35(12), 1950-1953[120W Ultra Light Solar Blanket Panel for Camping]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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