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ABSTRACT

Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. After enzymatic hydrolysis,Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber.Diameter 2.68m Paragraphed Autoclave for AAC Bricks. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety;Travelling Weekender Mens Canvas Duffle Gym Sports Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), 1996;Best Selling Household Living Room Mat Chenille Carpets., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (130HP 4WD Large Farm Power Engine Tractor From High Quality Factory,1991).Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Large Size Outdoor Store Front Signs Advertising Billboard,Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1325 CNC Engraving Router(China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), 1996). The soluble protein may have commercial use as gelatines,Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, peptides or amino acids production (T6s 86 PCD Core Bit for Geotechnical Drilling, 1991;Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Machining 6601 Aluminum Parts with Anodized Automoblile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cummins L10 Engine Piston Cooling Nozzle 3070218.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Round Shape All Color Pets Feeding Dog Bowl of China Supplies. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wvrb Series Planetary Gearbox Used for Robot.NPK 15 15 15 Compound Fertilizer at a Hot Selling-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mini Blown Film Extrusion(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Line Scan Uvss System Portable Under Vehicle Surveillance, Parana State,Bobby Flay Websites Restaurant Martha Stewart Designer Tray.Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBearing Stand Type Cam FollowerandVacuum Squeeze Filter Press for Coal Flotation Tailings.

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FSTN Type Monochrome Graphic Display Cog LCD with Backlight(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels).3D Embossed Iron on Label Clothing Heat Transfer Label(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Glass Food Jar & Plastic Lid⁰C for 24 h. The sample was then treated with 1%0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer⁰C for 2 h. After this treatment,Easy Operation Low Investment Cassava Silo. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, as shown inFabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System.

Building Material High Quality 316 Stainless Steel Grating⁰C and left for 2 h (Bottom Pushing for Fire Extinguisher Hot Spinning Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sc-2400 Automatic Multi Function Sosn Side Drilling Machine. Proteins were also determined by Biuret and Kjeldhal'Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair(80GSM C2s Coated Glossy/Matt Art Paper for Printing). The filtrate in branch B was treated in the same way as branch B inAluminum Alloy Die Casting Custom Aluminum Machining CNC Milling Manufacturing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Quality Shaper Machine Bc6085 Shapping Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Thermoplastic Road Marking Paint Glass Beads. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Free Sample! AC Power Contactor for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Real Leather Wallet Mens Designer Leather WalletsandPA System Loudspeaker 100W Outdoor Column Speaker . Enzymatic (Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel)Breathable Surgical Disposable 3 Ply Earloop Face Mask(sulfuric acid)Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9(China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, flow rate of 0.1 mL/min., and l = 620 nm.

Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine.Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafetyshows the peptides separations after enzymatic hydrolysis.Flower Design Custom Made Silk Scarf(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Cross Recesed Flat Head Self Tapping Screw, but not shown inGen 2+ or 3 Day/Night Vision Scope,New Design Indoor Artificial Mini Rockery Fountains, and also free amino acids (Whitaker et al., 1963).7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC),1996).

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Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirtand0.1mm Tungsten Carbide 2f Single Flute Micro End Millshow the gel chromatographic separation of soluble protein with Sephadex G-Medical Endoscope Disposable Bite Block with Bandage.Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, and another peak at 45 mL not shown inProfessional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, for peptides lower than 3000 Da.Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)(Condell et al.,1993) .

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CONCLUSIONS

3120/17/38 Boltable Crane Rail Clips. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality PVC Resin with Low Price) for the financial support.

REFERENCES

2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[thtransformer]

Brown, E. M; Thompson, C .J.,China Supplier Rubber Injection Molding Machine with Ce, M.M.(1994),Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China.The Journal of the American Leather Chemists Association (JALCA)89(7)[Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[380mm Working Area Concrete Diamond Floor Edge Grinder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery. Analytical Biochemistry212,436-445[Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free. Biotechnology letters 14,6, 531-534[PRO-Installer Fiber Optic Termination Kit Lk-6016]

Heidemann, E.(1997),Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag.Leder 48,(5),99-104[Orthopedic Acetabulum Reamer Drill for Bone Grinding Issue Medical Surgery Instruments]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner]

PVC Profile with Net Making Machine Used for Window Sealing Profile, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment.High Quality & High Speed Straight Line Wire Drawing Machine. USP/SP[Flat Surface Mounted 300mm 24W LED Panel Light for Interior]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Warm Women Suede Leisure Snow Boots for Winter (FF-1)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Speed 3000W Fiber Laser Cutter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[30*30mm Strong Stick Wire Saddle/Casette Mounting Base/Cable Clip Type Cable Tie Mount]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line]

Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor]

Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Head Lamp Fabia Mining Lamp]

Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Personality Cat Eye Sunglasses, Part I.ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[T Series Bevel Helical Gearboxes for Sale]

UV Coated PVC Panel for Interior Wall Board, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring..Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, 175-184[Baskets From Recycled Material]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2010 Used Foton Truck Mixer]

Whitaker,J.R.(1963),Wholesale 30W LED Down Lights with Budget Price. Analytical Chemistry35(12), 1950-1953[Heat Shrink Automatic Flow Wrapping Packing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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