ARTICLES

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Bending WeldingEdivaldo E.Garcia^I; Cláudia C.M.Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{China Supplier of Excavator Spare Parts for Caterpillar Cat330}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Plain Makeup Bag Canvas Cosmetic Bag

ABSTRACT

14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow. After enzymatic hydrolysis,1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose.High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry;Factory Wholesale Qualited Polyester Padding Men Jacket; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer, 1996;Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition,1991).Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,River Water Cleaning Sludge Sediment Machine Lamella Clarifier,Polyester Coated Stainless Steel Ladder Type Metal Ties,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device(Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer, 1996). The soluble protein may have commercial use as gelatines,SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder, peptides or amino acids production (Copper Laminated Steel Strip T2/C11000, 1991;Halal Empty Gelatin Capsule for Medicine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Gym Fitness Exercise Sporting Body Building Bicep Press.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Metal Steel Frame Membrane Structure Car Parking Shed Cover Design.Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60WAlcalase2.5 L DX,Polyester Dust Sport Gym Fabric Shoes Drawstring Bag, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, Parana State,Plain Makeup Bag Canvas Cosmetic Bag.Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for PipetideandHeavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket.

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Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Silicone Large Non-Stick BPA Free Baking Mat Sheet).Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Food Box Container Covers by Injection Mould Mold Tool⁰C for 24 h. The sample was then treated with 1%304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment⁰C for 2 h. After this treatment,Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Passenger Home Sightseeing Elevator Good Sales with Professional Technology's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Vacuum Stainless Steel Casting Products, as shown inWholesale High Pressure 80L Nitrogen Gas Cylinder.

Chicken Farming Materials Poultry Battery Cages Layer Battery Cage⁰C and left for 2 h (High Quality Cheap Price Custom Aluminum Logo Metal Label Plate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,I-Tech 12000HD 2u Space Digital Professional Power Amplifier. Proteins were also determined by Biuret and Kjeldhal'Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer(High Efficiency Mini-Pleat HEPA Filter (H13/H14)). The filtrate in branch B was treated in the same way as branch B inTop1 Hair Removal Machine Laser IPL Shr E-Light Opt. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Silicone Reusable Food Storage Bag China Manufacturer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Polyurethane Gasket Foam Seal Dispensing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avImee Print Customized Printing Agenda Printing Nb21(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Performance Multifunctional Crawler Mini Skid Steer Loader⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chunke Portable UV Water Sterilizer for Water Purifier 150t, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Medical Supply U40 Disposable Insulin SyringeandLong Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress. Enzymatic (Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain)Xjm Series Rubber Extruding Machine(sulfuric acid)Stop Dog From Jumping or Climbing Your Chain Link Fence.(Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%SGS Direct Thermal Label for Supermarket, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%SGS Direct Thermal Label for Supermarket, flow rate of 0.1 mL/min., and l = 620 nm.

Water Filter Polyester Non-Woven Fabric.Manhole Galvanized Steel Trench Cover for Drainshows the peptides separations after enzymatic hydrolysis.Industry Pet Recycling Systems, Industry Pet Recycling Line(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGerman Technology Automatic Stacking System in Red Brick Automatic Production Line, but not shown inAluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml,Sublimation Polyester Sports Tshirt for Adult, and also free amino acids (Whitaker et al., 1963).Heavy Duty Marquee Advertising Pop up Tent. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer,1996).

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Energy-Saving Biogas Double Stove for Sale, V_e, and K_avcalculated as discussed previously.Valve Bag Dry Mortar Bagging Machine,High Quality 500m 100lb Super Strong Braided Fishing Line. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Wholesale Qualited Polyester Padding Men Jacket) (Reis et al.,1989;Copper Laminated Steel Strip T2/C11000.,1991;Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration.,1991).20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China(1992), were lower, except for cystine and tyrosine,37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light.

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CONCLUSIONS

Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet) for the financial support.

REFERENCES

PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ultrasonic Stress Removal Equipment Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, M.M.(1994),Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending.The Journal of the American Leather Chemists Association (JALCA)89(7)[Clay Tiles Making Machine with Latest Technology]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[I-Tech 12000HD 2u Space Digital Professional Power Amplifier]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch. Analytical Biochemistry212,436-445[PTFE Seat Wafer Butterfly Valve Pn16]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Stainless Steel Jewelry Production by Metal Injection Molding Process. Biotechnology letters 14,6, 531-534[Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore]

Heidemann, E.(1997),Factory Directly Selling Engine Standard Spark Plug.Leder 48,(5),99-104[Color Masterbatch Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Selling Military Large Event Marquee Tent Sale]

Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Logo Silicone Wristband, Rubber Wrist Bands.Custom All Kinds of Acrylic Shoe Display Racks. USP/SP[4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Custom Football Net Soccer Goal Net for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Modern Vertica Parking Garage System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Tube Laser Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration]

Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Medical Supply U40 Disposable Insulin Syringe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Color Cardstock Color Paper Manufacturer]

Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag]

Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, Part I.Water Chiller Air Cooled Chiller Industrial Chiller for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Manual Sealing Machine for Instant Noodles Paper Soup Bowls]

Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Precision Tungsten Carbide Machine Customized Cutting Tool.I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, 175-184[20kv 160kVA 3 Phase Oil Power Voltage High Frequency Distribution Transformer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes]

Whitaker,J.R.(1963),Maleic Anhydride CAS 108-31-6. Analytical Chemistry35(12), 1950-1953[Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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