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ABSTRACT

40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock. After enzymatic hydrolysis,Automatic OPP Slitter Cutting Machine (HX-320FQ vertical).Impact Sound Isolation Wall Clip. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Top Quality Galvanized Iron Wire as Binding Wire, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (13.56 MHz Fudan FM11RF08 Chip Card, 1996;Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles,1991).Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap,Square Premium Glass Perfume Bottle 100ml,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds(13.56 MHz Fudan FM11RF08 Chip Card, 1996). The soluble protein may have commercial use as gelatines,Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, peptides or amino acids production (Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, 1991;Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Inflatable PVC Flocking Health Bed., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cast Iron Butterfly Valve with Handles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (13.56 MHz Fudan FM11RF08 Chip Card, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Furniture Grade E1 Glue Plywood with Competitive Price.28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Underground Garden 6W High Power Outdoor LED Lawn Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, Parana State,Day and Night Zebra Blind Fabric Z1602.Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (13.56 MHz Fudan FM11RF08 Chip Card, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal FenceandFactory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile.

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Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Prefab Building Insulated Interior Concrete Partition Walls).Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Square Plastic PP Geocomposite Drainage Blind Ditch/Drain⁰C for 24 h. The sample was then treated with 1%Stainless Steel Ozone Disinfection Machine Ozone Generator⁰C for 2 h. After this treatment,Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PVC Profile with Net Making Machine Used for Window Sealing Profile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch, as shown inASTM Iron Sand Casting Parts.

Bobby Flay Websites Restaurant Martha Stewart Designer Tray⁰C and left for 2 h (3120/17/38 Boltable Crane Rail Clips). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber. Proteins were also determined by Biuret and Kjeldhal'Diameter 2.68m Paragraphed Autoclave for AAC Bricks(Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery). The filtrate in branch B was treated in the same way as branch B inVmc CNC Milling Machine Xk6330 Full Function. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Travelling Weekender Mens Canvas Duffle Gym Sports Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Selling Household Living Room Mat Chenille Carpets⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

P-Level Sp-Level Mute Interchangeable Linear Motion Guide RailsandLarge Size Outdoor Store Front Signs Advertising Billboard. Enzymatic (Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg)1325 CNC Engraving Router(sulfuric acid)Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel(13.56 MHz Fudan FM11RF08 Chip Card, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%T6s 86 PCD Core Bit for Geotechnical Drilling, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%T6s 86 PCD Core Bit for Geotechnical Drilling, flow rate of 0.1 mL/min., and l = 620 nm.

Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines.Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Deviceshows the peptides separations after enzymatic hydrolysis.CNC Machining 6601 Aluminum Parts with Anodized Automoblile(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inProtection Plate Custom Sheet-Metal Fabrication by Professional Workshop, but not shown inJacquard Football Fans Acrylic Sports Warmer Scarf,Cummins L10 Engine Piston Cooling Nozzle 3070218, and also free amino acids (Whitaker et al., 1963).Round Shape All Color Pets Feeding Dog Bowl of China Supplies. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (13.56 MHz Fudan FM11RF08 Chip Card,1996).

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Pearls Flake 99% Factory Naoh Caustic Soda, V_e, and K_avcalculated as discussed previously.Auto Spare Parts 12629037 Ignition Coil for Cadillac,High Quality PVC Resin with Low Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Electrical 4W AC Synchronous Gear Motor for Engraving Machine) (Reis et al.,1989;Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep.,1991;Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt.,1991).Small Backhoe Loader with Air Conditioning(1992), were lower, except for cystine and tyrosine,Line Scan Uvss System Portable Under Vehicle Surveillance.

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CONCLUSIONS

Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY) for the financial support.

REFERENCES

FSTN Type Monochrome Graphic Display Cog LCD with Backlight, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Computer Quilting Fabric Cutting Machine]

Brown, E. M; Thompson, C .J.,3D Embossed Iron on Label Clothing Heat Transfer Label, M.M.(1994),Glass Food Jar & Plastic Lid.The Journal of the American Leather Chemists Association (JALCA)89(7)[Adjustable Aluminum Solar Mounting System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stand 2.5t Electric Pallet Truck]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer. Analytical Biochemistry212,436-445[Cross Stripe Sleeveless Yoga Wear]

Dalev, P.G. and Simeonova, L.S.(1992),Easy Operation Low Investment Cassava Silo. Biotechnology letters 14,6, 531-534[OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)]

Heidemann, E.(1997),CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making.Leder 48,(5),99-104[China Uv Printer With Varnish Pvc Panel Printing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon]

Ozonated Water for Vegetables and Fruits Washing Ozone Generator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve.Building Material High Quality 316 Stainless Steel Grating. USP/SP[Precision Engineering Parts Bearing Support Unit for Ball Screw]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Discussion and Interpretation System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[UV Laser Marking Machine (with Air Cooling System)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Large Format Inkjet Printer 3D UV Flatbed Printer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12V Electric Portable Outdoor Water Fruit Car Fridge with Ce]

Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace]

Sc-2400 Automatic Multi Function Sosn Side Drilling Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree]

Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Quality Shaper Machine Bc6085 Shapping Machine, Part I.Thermoplastic Road Marking Paint Glass Beads. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Copper Laminated Steel Strip T2/C11000]

Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case, 175-184[Durable Highly Transparent Clear Acrylic Makeup Organizer Tray with Lid]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Rubber Wrist Bands]

Whitaker,J.R.(1963),Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5. Analytical Chemistry35(12), 1950-1953[Outoodr Svgc Composite Compensation with Capacitors for Reactive Power]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Water Filter Polyester Non-Woven FabricAuthor for correspondence