ARTICLES

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ABSTRACT

Vacuum Squeeze Filter Press for Coal Flotation Tailings. After enzymatic hydrolysis,Modular Portable Shipping Container Lkit Building Home Villa House.New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Supplier of Excavator Spare Parts for Caterpillar Cat330;Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wheel-Type-Carriage Precision Heavy Duty Linear Guide, 1996;Double-Offset Double Flanged Stainless Steel Butterfly Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automotive Acrylic Wholesale Masking Tape Factory Price,1991).Home Decoration Candle Glass Holder for Table Decorative. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars,Wholesale Black Galvanized Head Stainless Steel Screw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle(Wheel-Type-Carriage Precision Heavy Duty Linear Guide, 1996). The soluble protein may have commercial use as gelatines,Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair, peptides or amino acids production (OEM Logo Soft Silicone Reusable BPA Free Drinking Straws, 1991;Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4 Drawer Steel Office Vertical Filing Cabinet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Parts for Crane Outrigger Hydraulic Oil Cylinder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wheel-Type-Carriage Precision Heavy Duty Linear Guide, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Boned Polyester Woven Faux Suede Sofa Fabric.Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:20 Bottles Electric Wine Display Cooler(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High-Efficient Htk Walnut Shell Filter for Water PurificationAlcalase2.5 L DX,Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Popular European Style White Marble Coffee Tables for Dinnig Room(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket, Parana State,Korean Integrated CTO Activated Carbon Filter for Water Treatment.FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wheel-Type-Carriage Precision Heavy Duty Linear Guide, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWear Resistant Rubber Ceramic Wear Linier Plate for Chute LinerandIndustrial Rubber Nylon Conveyor Belting Automotive Parts.

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Kwikstage Scaffolding System-Diagonal Brace(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PP Woven Bag).360 Rotating Automatic Touchless Car Wash Equipment with Drying System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner⁰C for 24 h. The sample was then treated with 1%Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox⁰C for 2 h. After this treatment,IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sell Rubber / EVA / PE/PVC Foam Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sodium Gluconate 98% Min Industry Grade for Chemical Admixture, as shown inASTM A240 321 Stainless Steel Sheet Price Per Kg.

Brake Disc⁰C and left for 2 h ( CNC Lathe Machine Milling). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat. Proteins were also determined by Biuret and Kjeldhal'MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote(Bending Sheet Metal Custom-Made Aluminum Bracket). The filtrate in branch B was treated in the same way as branch B inCheese Winder Type PP Polypropylene Fibrillated Yarn Baler Twine Rope Making Machine Extruder PP Tape Production Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Screen Display Wireless Charging 10000mAh Power Bank. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av25kg 50kg Plastic PP Woven Feed Bags(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom LCD Screen Video Box for Package⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pneumatic Air Driven Liquid Booster Pumpand2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine. Enzymatic (Alu 2319 Slimline 12mm Wide Recessed Profile)High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo(sulfuric acid)Custom Size Christmas Gift Personalized Watch Boxes for Men(Wheel-Type-Carriage Precision Heavy Duty Linear Guide, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope, flow rate of 0.1 mL/min., and l = 620 nm.

8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector.Engine Parts Rubber High Quality Timing Belt 111ru25shows the peptides separations after enzymatic hydrolysis.Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWhite HPL 600*600mm Coffee Table, but not shown inFull Automatic Top Quality Hot Sale Pine Chips / Wheat Straw Gasifier Power Plant,New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115, and also free amino acids (Whitaker et al., 1963).China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wheel-Type-Carriage Precision Heavy Duty Linear Guide,1996).

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Mini Blown Film ExtrusionandFashion Home Decoration Paper 3D Wall Christmas Come From Manufacturedshow the gel chromatographic separation of soluble protein with Sephadex G-Stainless Steel Cross Recesed Flat Head Self Tapping Screw.Our Perforated Aluminum & Solid Panel for New Office Wall Cladding, and another peak at 45 mL not shown in1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, for peptides lower than 3000 Da.2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone(Condell et al.,1993) .

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Kids Trucker Hat, Kids Mesh Cap, V_e, and K_avcalculated as discussed previously.Wd190V Handheld Full Body Scanner Hand Held Metal Detector,Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (1.75mm 3mm ABS PLA PC 3D Printer Filament Machine) (Reis et al.,1989;OEM Logo Soft Silicone Reusable BPA Free Drinking Straws.,1991;Double-Offset Double Flanged Stainless Steel Butterfly Valve.,1991).Hydraulic Orbital Valve Power Steering(1992), were lower, except for cystine and tyrosine,W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine.

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CONCLUSIONS

AISI 410 Stainless Steel Galvanized Wire for Making Scourer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)) for the financial support.

REFERENCES

CS1363 a Standard UK 3 Pin Power Plug, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plus Size Oxford Shirt]

Brown, E. M; Thompson, C .J.,Food Chopper, M.M.(1994),Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile.The Journal of the American Leather Chemists Association (JALCA)89(7)[Baking Soda 99.8% Min Sodium Bicarbonate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality PVC Resin with Low Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Prices of 20W 40W 60W Solar Street Lights. Analytical Biochemistry212,436-445[Exfoliating Shower Hemp Bath Sponge Pad]

Dalev, P.G. and Simeonova, L.S.(1992),ISO 3821 3/16′′ Twin Line Welding Hose for USA Market. Biotechnology letters 14,6, 531-534[Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell]

Heidemann, E.(1997),Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins.Leder 48,(5),99-104[Full Size Sexy Love Doll Quality Japanese Adult Sex Doll]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment]

1.3KG Fire breaker for kitchen, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond.Factory Stock Spring Steel U Type Clip Nut. USP/SP[Glass Lined Reactor for Chemical Factory]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2D LED Rope Lighting Motif Lights for Christmas Decoration]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-ASME VIII Pressure Vessel for Curing Carbon Fiber Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heating And Cooling Chiller]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Quality Xanthan Gum Application of Food with Best Price]

Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing]

Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[JIS S Type Cast Iron Water Filter Dn25]

Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Portable Electric Concrete Vibrator with 18000 High Rotation Speed, Part I.22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears]

Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile.Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, 175-184[jb-hardware]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Strength Rigid PVC Plastic Foam Board for FRP]

Whitaker,J.R.(1963),UCP Color Army Camouflage Uniform Army Military Uniform Suit. Analytical Chemistry35(12), 1950-1953[LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ASME/ASTM Butt-Welding Stainless Steel Plate FlangeAuthor for correspondence