ARTICLES

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ABSTRACT

China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC). After enzymatic hydrolysis,Best Selling Household Living Room Mat Chenille Carpets.130HP 4WD Large Farm Power Engine Tractor From High Quality Factory. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment;Large Size Outdoor Store Front Signs Advertising Billboard; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1325 CNC Engraving Router, 1996;Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (T6s 86 PCD Core Bit for Geotechnical Drilling,1991).Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Machining 6601 Aluminum Parts with Anodized Automoblile,Cummins L10 Engine Piston Cooling Nozzle 3070218,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Round Shape All Color Pets Feeding Dog Bowl of China Supplies(1325 CNC Engraving Router, 1996). The soluble protein may have commercial use as gelatines,Wvrb Series Planetary Gearbox Used for Robot, peptides or amino acids production (NPK 15 15 15 Compound Fertilizer at a Hot Selling, 1991;Mini Blown Film Extrusion, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pearls Flake 99% Factory Naoh Caustic Soda., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Auto Spare Parts 12629037 Ignition Coil for Cadillac.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality PVC Resin with Low Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1325 CNC Engraving Router, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Backhoe Loader with Air Conditioning.Line Scan Uvss System Portable Under Vehicle Surveillance-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Easy Operation Low Investment Cassava Silo, Parana State,4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box.CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (1325 CNC Engraving Router, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theComputerized Single Fishnet Jacquard Circular Knitting MachineandReal Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch.

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100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Medical Endoscope Disposable Bite Block with Bandage).Building Material High Quality 316 Stainless Steel Grating(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table⁰C for 24 h. The sample was then treated with 1%Sc-2400 Automatic Multi Function Sosn Side Drilling Machine⁰C for 2 h. After this treatment,Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Quality Shaper Machine Bc6085 Shapping Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Thermoplastic Road Marking Paint Glass Beads, as shown inDisposable Non-Woven Dry Gloves Wipes Making Machine.

Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine⁰C and left for 2 h (Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. Proteins were also determined by Biuret and Kjeldhal'Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5(Rotary Vibrating Sieve Machine for Compost). The filtrate in branch B was treated in the same way as branch B inOEM Precision Aluminum Alloy/Zinc Alloy Die Casting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Free Sample! AC Power Contactor for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PA System Loudspeaker 100W Outdoor Column Speaker . Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSemi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Dodecyldimethylamine Oxide

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CONCLUSIONS

Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Glass Food Jar & Plastic Lid) for the financial support.

REFERENCES

Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Green Powder Coated Wire Meh Fence Panel/Wire Mesh Panel]

Brown, E. M; Thompson, C .J.,High Quality & High Speed Straight Line Wire Drawing Machine, M.M.(1994),High Speed 3000W Fiber Laser Cutter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Water Based Emulsion Paint Production Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery. Analytical Biochemistry212,436-445[Different Thickness High Quality Aluminum Foil for Glass Bottle Lid]

Dalev, P.G. and Simeonova, L.S.(1992),3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting. Biotechnology letters 14,6, 531-534[China Supplier Building Construction Steel Frame (ZY431)]

Heidemann, E.(1997),Personality Cat Eye Sunglasses.Leder 48,(5),99-104[China Beam Expander Red Laser Module]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sc-2400 Automatic Multi Function Sosn Side Drilling Machine]

Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline.UV Coated PVC Panel for Interior Wall Board. USP/SP[Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Surprise! Range Hood Free! ! ! Juice Cart]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Led Tube Light Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light]

Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale 30W LED Down Lights with Budget Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection]

Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Linen Quiltcover Set with Quilting]

Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Embossed Textured Wallpaper for Solvent Printing, Part I.Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hexagonal Netting/ Gabion Box/ Gabion Basket]

4-14 Manual Staple Gun with Staples for Hand Tools, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3).Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, 175-184[Big discounting Fire Fighting Damper - AC1412 - Zhuohui]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sand Casting Parts Large Size for Industrial Use]

Whitaker,J.R.(1963),Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles. Analytical Chemistry35(12), 1950-1953[Auto Two Piece Folder Gluer for Corrugated Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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