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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-4 Styles Blue Glass Crystal Butt Beads Spiral Plug. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve

ABSTRACT

Industrial Activated Carbon Water Filter Industry Water Treatment. After enzymatic hydrolysis,2016 New Generation Sausage Making Machine.Magnetic Mixer Mixing Tank with Bottom Agitator (MTK). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent;New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Supplier Building Construction Steel Frame (ZY431), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing, 1996;Lab Equipment portable Fume Hood., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt,1991).10ton Telescopic Hydraulic Truck Crane. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV,for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cracker Multi Rows Trayless Packaging Machine(iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing, 1996). The soluble protein may have commercial use as gelatines,Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China, peptides or amino acids production (2 Quick Change Flexible Roloc Disc, 1991;UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Aluminium Windows Thermal Break China Supplier., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PRO-Installer Fiber Optic Termination Kit Lk-6016.Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut, Parana State,Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve.Nylon Cable Glands with CE, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576)andQuality Shaper Machine Bc6085 Shapping Machine.

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X Shaped Extruded Aluminum Window Screen Frames(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (16mm 12 Strand UHMWPE Synthetic Fiber Rope).Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4⁰C for 24 h. The sample was then treated with 1%4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging⁰C for 2 h. After this treatment,22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Low Power Consumption Laser Foam Vertical Die Cutting Machine, as shown inOzonated Water for Vegetables and Fruits Washing Ozone Generator.

New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug⁰C and left for 2 h (Blue White Yellow Red Black Nylon Mosquito  Window Netting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity. Proteins were also determined by Biuret and Kjeldhal'H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)(Concrete Aluminum Formwork System Aluminium Formwork). The filtrate in branch B was treated in the same way as branch B in7 Quad Screen Monitor Car HD Backup Camera System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Crusher for Used Plastic Recycling Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wear Resitant Rope End Cap Plastic Flange Cover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Comfortable Two Piece Women Fitness Clothing for Gym Wear, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Galvanized Bar Gratingand2019 Summer Fresh Landscaping Turf Lawnshow the gel chromatographic separation of soluble protein with Sephadex G-81144m Thrust Roller Bearing Used for Vertical Machine Machinery.Fancy Veneer Plywood. Natural Veneer Plywood for Furniture, and another peak at 45 mL not shown inWholesale Custom 100% Cotton Boys T-Shirt with Print, for peptides lower than 3000 Da.OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating(Condell et al.,1993) .

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Aluminum Foil Heat Shield Screen Matting Adhesive Backed, V_e, and K_avcalculated as discussed previously.Home Kitchen Water Purification Ozone Generator 1000mg,PP Foam Stationary Sheet Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion) (Reis et al.,1989;2 Quick Change Flexible Roloc Disc.,1991;Lab Equipment portable Fume Hood.,1991).Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)(1992), were lower, except for cystine and tyrosine,Economical Residential Home Automatic Aluminum Rolling Shutter Door.

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CONCLUSIONS

Clear PVC Sheet with Coating for Card. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor) for the financial support.

REFERENCES

Classic Black and White Plaid Sling Bag for Women, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PVC Electric Wire Plastic Cover PVC Electrical Jacket Sleeve]

Brown, E. M; Thompson, C .J.,24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, M.M.(1994),10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Size Christmas Gift Personalized Watch Boxes for Men]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Free Sample E Liquid/E-Juice for Nicotine Vaping. Analytical Biochemistry212,436-445[Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines]

Dalev, P.G. and Simeonova, L.S.(1992),Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft. Biotechnology letters 14,6, 531-534[Automotive Perforated Metal Stamping Part for Furniture]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fancy Veneer Plywood. Natural Veneer Plywood for Furniture]

High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Prefab Steel Structure Shed for Storage Warehouse.Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture. USP/SP[Best Design and Nice Price All in One Solar Street Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gen 2+ or 3 Day/Night Vision Scope: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gilets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner]

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Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed]

Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1650mm*80mm Stable Power Laser Tube, Part I.FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Step Motor for Intelligent Sanitary/3D Printer]

High Quality Lead Ingot 99.994 Reasonable Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Micro-Combustion Tube, Test Tube (HK-224).Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen, 175-184[Modular Building]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag]

Whitaker,J.R.(1963),OEM Service Machining Milling Turning Service Custom CNC Aluminum Case. Analytical Chemistry35(12), 1950-1953[Swimming Pool Water Disinfect Ozone Generator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Tpo Waterproof Membrane/Thermoplastic Polyolefin for RooofingAuthor for correspondence