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ABSTRACT

SGS Direct Thermal Label for Supermarket. After enzymatic hydrolysis,Water Filter Polyester Non-Woven Fabric.Industry Pet Recycling Systems, Industry Pet Recycling Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1996;Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Energy-Saving Biogas Double Stove for Sale,1991).Valve Bag Dry Mortar Bagging Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality 500m 100lb Super Strong Braided Fishing Line,20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light(Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1996). The soluble protein may have commercial use as gelatines,Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, peptides or amino acids production (PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, 1991;Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Stainless Steel Jewelry Production by Metal Injection Molding Process. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Directly Selling Engine Standard Spark Plug.Custom Logo Silicone Wristband, Rubber Wrist Bands-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom All Kinds of Acrylic Shoe Display Racks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Precision Tungsten Carbide Machine Customized Cutting Tool(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Chicken Farming Materials Poultry Battery Cages Layer Battery Cage).I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Maleic Anhydride CAS 108-31-6⁰C for 24 h. The sample was then treated with 1%2000mj Q Switched for Pigment Removal with Ce Certification⁰C for 2 h. After this treatment,Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cow Dung Chicken Manure Organic Fertilizer Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)marble Makeup Brush Set for Travel in 20PCS, as shown inOPP Printed Block Bottom Bags.

Swimming Pool Water Disinfect Ozone Generator⁰C and left for 2 h (Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.20kv 50kVA High Voltage Dry Type Power Supply Transformer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp. Proteins were also determined by Biuret and Kjeldhal'High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter(High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump). The filtrate in branch B was treated in the same way as branch B inColorful Extension Cord For Iron. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4 Styles Blue Glass Crystal Butt Beads Spiral Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndustrial Activated Carbon Water Filter Industry Water Treatment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Medical Supply U40 Disposable Insulin Syringe) for the financial support.

REFERENCES

Powder Coated H Column Beam Steel Structure Building for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Photovoltaic Industry CNC Internal Grinding Machine Tool Mk2120]

Brown, E. M; Thompson, C .J.,Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, M.M.(1994),Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut. Analytical Biochemistry212,436-445[Top China Forklift Supplier & Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),Nylon Cable Glands with CE. Biotechnology letters 14,6, 531-534[Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box]

Heidemann, E.(1997),X Shaped Extruded Aluminum Window Screen Frames.Leder 48,(5),99-104[bnpo3]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Laser Projector RGB Laser Light for Stage]

Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair.Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4. USP/SP[Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Axial Magnetized Special Shaped Magnet Products Made in China]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ID25mm Od86mm Standard Through Hole Slip Ring]

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22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door]

22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Low Power Consumption Laser Foam Vertical Die Cutting Machine, Part I.New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Comfortable Two Piece Women Fitness Clothing for Gym Wear]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox]

Whitaker,J.R.(1963),Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet. Analytical Chemistry35(12), 1950-1953[Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)Author for correspondence