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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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SGS Direct Thermal Label for Supermarket. After enzymatic hydrolysis,Water Filter Polyester Non-Woven Fabric.Industry Pet Recycling Systems, Industry Pet Recycling Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sublimation Polyester Sports Tshirt for Adult;Heavy Duty Marquee Advertising Pop up Tent; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1996;Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Energy-Saving Biogas Double Stove for Sale,1991).Valve Bag Dry Mortar Bagging Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality 500m 100lb Super Strong Braided Fishing Line,20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light(Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1996). The soluble protein may have commercial use as gelatines,Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, peptides or amino acids production (PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, 1991;Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Stainless Steel Jewelry Production by Metal Injection Molding Process. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Factory Directly Selling Engine Standard Spark Plug.Custom Logo Silicone Wristband, Rubber Wrist Bands-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom All Kinds of Acrylic Shoe Display Racks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Water Chiller Air Cooled Chiller Industrial Chiller for Sale, Parana State,Stop Dog From Jumping or Climbing Your Chain Link Fence..Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGyc 2000L 100L Alcohol Liquor and Spirits Distilling PlantandCustomized Large Ss Stamping/ Bending Sheet Metal Parts.

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High Precision Tungsten Carbide Machine Customized Cutting Tool(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Chicken Farming Materials Poultry Battery Cages Layer Battery Cage).I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Maleic Anhydride CAS 108-31-60C for 24 h. The sample was then treated with 1%2000mj Q Switched for Pigment Removal with Ce Certification0C for 2 h. After this treatment,Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cow Dung Chicken Manure Organic Fertilizer Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)marble Makeup Brush Set for Travel in 20PCS, as shown inOPP Printed Block Bottom Bags.

Swimming Pool Water Disinfect Ozone Generator0C and left for 2 h (Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.20kv 50kVA High Voltage Dry Type Power Supply Transformer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp. Proteins were also determined by Biuret and Kjeldhal'High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter(High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump). The filtrate in branch B was treated in the same way as branch B inColorful Extension Cord For Iron. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4 Styles Blue Glass Crystal Butt Beads Spiral Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavIndustrial Activated Carbon Water Filter Industry Water Treatment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Hot Sale Electric LED light Aroma DiffuserandChina Supplier Building Construction Steel Frame (ZY431). Enzymatic (iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing)Lab Equipment portable Fume Hood(sulfuric acid)Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt(Fire Extinguisher UV Curing Machine Cylinder Drying Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%10ton Telescopic Hydraulic Truck Crane, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%10ton Telescopic Hydraulic Truck Crane, flow rate of 0.1 mL/min., and l = 620 nm.

Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV.Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vestshows the peptides separations after enzymatic hydrolysis.for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPolycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer, but not shown inISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill,Cracker Multi Rows Trayless Packaging Machine, and also free amino acids (Whitaker et al., 1963).Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fire Extinguisher UV Curing Machine Cylinder Drying Machine,1996).

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Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines, Ve, and Kavcalculated as discussed previously.2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01,PRO-Installer Fiber Optic Termination Kit Lk-6016. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket) (Reis et al.,1989;PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint.,1991;Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set.,1991).Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace(1992), were lower, except for cystine and tyrosine,Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901).

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Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


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Powder Coated H Column Beam Steel Structure Building for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Photovoltaic Industry CNC Internal Grinding Machine Tool Mk2120]

Brown, E. M; Thompson, C .J.,Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, M.M.(1994),Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut. Analytical Biochemistry212,436-445[Top China Forklift Supplier & Manufacturer]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Laser Projector RGB Laser Light for Stage]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Axial Magnetized Special Shaped Magnet Products Made in China]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox]

Whitaker,J.R.(1963),Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet. Analytical Chemistry35(12), 1950-1953[Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)Author for correspondence

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