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ABSTRACT

Micro-Combustion Tube, Test Tube (HK-224). After enzymatic hydrolysis,Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stationery PP Clipboard With 7 Inch Scale Ruler, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box, 1996;Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PVC Cross Joint Pipe Fittings Equal Pipe Cross,1991).3PC Stainless Steel Flanged End Ball Valve 3 Inches 150. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ysdcnc Manual Sheet Metal Plate Bending Roller Machine,Betaine Anhydrous 98% Food Grade Good Quality Competitive Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover(Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box, 1996). The soluble protein may have commercial use as gelatines,Bearing Stand Type Cam Follower, peptides or amino acids production (Hot Sale Electric LED light Aroma Diffuser, 1991;Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-7PCS RGBW LED Plastic PAR Light Wall Washer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm.Kindergarten Playground Preschool Kids Plastic Slide and Swing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sud1000h Poly Welder Butt Fusion Machine).SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes⁰C for 24 h. The sample was then treated with 1%Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut⁰C for 2 h. After this treatment,0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)304 316 Stainless Steel Grooved Cross for Water, as shown inAustralian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers.

Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole⁰C and left for 2 h (Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Laser Projector RGB Laser Light for Stage(Activated Carbon Strong Effect Peeling off Face Mask Mud). The filtrate in branch B was treated in the same way as branch B inHigh Quality Vertical Type Fully Auto Alcohol Swab Pad Making Machinery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAcm Dewatering Twin Screw Extruder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tumble Belt Type Shot Blasting Machine for Iron Castings Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Quality Good Price China Plastic Injection Molding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ground Finish Molybdenum Tzm Rod, V_e, and K_avcalculated as discussed previously.Transparent/Clear BOPP Jumbo Roll,Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Buy Single Item Moving Bee Eye Light Lowly Price) (Reis et al.,1989;Hot Sale Electric LED light Aroma Diffuser.,1991;Custom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle.,1991).Chenille Microfiber Mop Head(1992), were lower, except for cystine and tyrosine,Custom Waterproof Pizza Sign Large Advertising Signboard.

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CONCLUSIONS

PVC Shrink Sleeve Label for Drinking Water Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rainsky Ceiling Mounted Stainless Steel Shower Heads) for the financial support.

REFERENCES

Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Disposable Blood Lancet with CE&ISO Certification (MT58054006)]

Brown, E. M; Thompson, C .J.,Galvanized Square Wire Mesh, Metal Window Screen, M.M.(1994),Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[U. S. Type Drop Forged Wire Rope Clip]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter. Analytical Biochemistry212,436-445[Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Customized Stainless Steel Parts by Deep Drawing. Biotechnology letters 14,6, 531-534[SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)]

Heidemann, E.(1997),Children Bear English Story Book Printing.Leder 48,(5),99-104[Apple Earbuds Review]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12ml Round Shape Empty Clear Nail Polish Glass Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cherry Wooden Grain PVC Film in Furniture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Diamond Segments 2000mm Diameter Granite Cutting Blade]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Fashion Design Wholesale Deluxe OEM Pet House Cat Climbing Tree]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 New Advertising Inflatables Air Walking Backpack Ball]

Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer]

Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ FDA Certificated]

Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), Part I.Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Large Batches Equipoise Light Yellow Liquids]

Whitaker,J.R.(1963),Teerte Square Flat Head Self Tapping Screw. Analytical Chemistry35(12), 1950-1953[Large Size Outdoor Store Front Signs Advertising Billboard]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Machining Metal/Aluminum/Brass Auto PartsAuthor for correspondence