ARTICLES

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ABSTRACT

Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5. After enzymatic hydrolysis,Free Sample! AC Power Contactor for Sale.PA System Loudspeaker 100W Outdoor Column Speaker . Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, 1996;Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flower Design Custom Made Silk Scarf,1991).New Design Indoor Artificial Mini Rockery Fountains. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane,Medical Endoscope Disposable Bite Block with Bandage,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels(Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, 1996). The soluble protein may have commercial use as gelatines,Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), peptides or amino acids production (Ladder Type Cable Tray with Ce/ GOST/ TUV/UL, 1991;12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fine Bubble Diffuser for Air Purifier., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3120/17/38 Boltable Crane Rail Clips.2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Supplier Rubber Injection Molding Machine with Ce(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Speed 3000W Fiber Laser Cutter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rectangular Stainless Steel Pipe SS304).Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting⁰C for 24 h. The sample was then treated with 1%Personality Cat Eye Sunglasses⁰C for 2 h. After this treatment,ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)UV Coated PVC Panel for Interior Wall Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring., as shown inStainless Steel Ozone Disinfection Machine Ozone Generator.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmallest Portable Waste Transformer Oil Purifier Machine Zy-6(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed Automatic Packing Machine (GK-650 BA)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/5000-5900MHz 4G Built-in Automotive Antenna, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Glass Lined Reactor for Chemical Factory, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Explosion Proof Fog Cannon for Coal Plant. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free) for the financial support.

REFERENCES

Silicone Large Non-Stick BPA Free Baking Mat Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.wetroyes.com]

Brown, E. M; Thompson, C .J.,19mic Black Cast PE Stretch Film for Pallet Wrapping, M.M.(1994),Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens.The Journal of the American Leather Chemists Association (JALCA)89(7)[Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Betaine Anhydrous 98% Food Grade Good Quality Competitive Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Design Plush Panda Animal Slippers Comfort Foam Support by. Analytical Biochemistry212,436-445[Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree]

Dalev, P.G. and Simeonova, L.S.(1992),Best 6-Axis Electric Screwdriver Machine Robot. Biotechnology letters 14,6, 531-534[High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo]

Heidemann, E.(1997),Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts.Leder 48,(5),99-104[Stainless Steel Quarter Round Corner Tile Trim]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Explosion Proof Fog Cannon for Coal Plant]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Multi-Function Small Tree Digging Shovel/Tree Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sodium Gluconate 98% Min Industry Grade for Chemical Admixture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbide Cnc Router Bit Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Alumina Ball with High Quality]

Automatic L Bar Sealer Shrink Tunnel Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full Automatic Carbonated Water Filling Full Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Automotive Water Pump Seal]

Automatic L Bar Sealer Shrink Tunnel Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable]

Automatic L Bar Sealer Shrink Tunnel Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile, Part I.Superior Watertightness JS Polymer Cement Waterproof Coating for Basement. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs]

PS 200 Upper Discharge Centrifuge, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew.Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, 175-184[2oz 3oz 4oz 6oz 8oz 12oz 16oz Bid Wide Mouth Straight Kitchen Empty Tea Food Bamboo Lid Storage Glass Jars]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing]

Whitaker,J.R.(1963),Electrosurgical Generator High Frequency Electricity Kinfe. Analytical Chemistry35(12), 1950-1953[Eco-Friendly Recycling PS Foam Container Production Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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