ARTICLES

Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light

Anti Flame Aramid SuitEdivaldo E.Garcia^I; Cláudia C.M.Round Casting Iron Trench Cover Manhole Cover Frame^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Bottom Pushing for Fire Extinguisher Hot Spinning Machine}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Eco-Friendly Aluminum Mechanical Part, CNC Machining

ABSTRACT

Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench. After enzymatic hydrolysis,Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table.Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric;Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ductile Iron Flanged Swing Check Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ozonated Water for Vegetables and Fruits Washing Ozone Generator, 1996;Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Day and Night Zebra Blind Fabric Z1602,1991).40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic OPP Slitter Cutting Machine (HX-320FQ vertical),Impact Sound Isolation Wall Clip,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving(Ozonated Water for Vegetables and Fruits Washing Ozone Generator, 1996). The soluble protein may have commercial use as gelatines,German Technology Automatic Stacking System in Red Brick Automatic Production Line, peptides or amino acids production (Top Quality Galvanized Iron Wire as Binding Wire, 1991;13.56 MHz Fudan FM11RF08 Chip Card, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ozonated Water for Vegetables and Fruits Washing Ozone Generator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap.Square Premium Glass Perfume Bottle 100ml-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, Parana State,Eco-Friendly Aluminum Mechanical Part, CNC Machining.Cast Iron Butterfly Valve with Handles, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ozonated Water for Vegetables and Fruits Washing Ozone Generator, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the80GSM C2s Coated Glossy/Matt Art Paper for PrintingandReal Leather Wallet Mens Designer Leather Wallets.

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Furniture Grade E1 Glue Plywood with Competitive Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Cross Recesed Flat Head Self Tapping Screw).28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Underground Garden 6W High Power Outdoor LED Lawn Light⁰C for 24 h. The sample was then treated with 1%Stamping Metal Parts Manufacturer Sheet Parts Fabrication⁰C for 2 h. After this treatment,Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hexagonal Netting/ Gabion Box/ Gabion Basket, as shown inGen 2+ or 3 Day/Night Vision Scope.

Different Thickness High Quality Aluminum Foil for Glass Bottle Lid⁰C and left for 2 h (Stop Dog From Jumping or Climbing Your Chain Link Fence.). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. Proteins were also determined by Biuret and Kjeldhal'Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration(Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt). The filtrate in branch B was treated in the same way as branch B inFood Grade Hose Home Depot PVC Clear Hose for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Square Plastic PP Geocomposite Drainage Blind Ditch/Drain.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Ozone Disinfection Machine Ozone Generator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSquare Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bobby Flay Websites Restaurant Martha Stewart Designer Tray, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

0.1mm Tungsten Carbide 2f Single Flute Micro End MillandAirport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber. Enzymatic (Diameter 2.68m Paragraphed Autoclave for AAC Bricks)Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety(sulfuric acid)Travelling Weekender Mens Canvas Duffle Gym Sports Bag(Ozonated Water for Vegetables and Fruits Washing Ozone Generator, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box, flow rate of 0.1 mL/min., and l = 620 nm.

China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC).Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mouldshows the peptides separations after enzymatic hydrolysis.Best Selling Household Living Room Mat Chenille Carpets(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Army Captain Navy Blue Color Hat for Army Tender, but not shown inElectric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor,130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, and also free amino acids (Whitaker et al., 1963).Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ozonated Water for Vegetables and Fruits Washing Ozone Generator,1996).

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China Excavator Radiator /Oil Radiator /Water Radiator ManufactureandPromotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthdayshow the gel chromatographic separation of soluble protein with Sephadex G-Large Size Outdoor Store Front Signs Advertising Billboard.Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, and another peak at 45 mL not shown in380mm Working Area Concrete Diamond Floor Edge Grinder, for peptides lower than 3000 Da.1325 CNC Engraving Router(Condell et al.,1993) .

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Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel, V_e, and K_avcalculated as discussed previously.T6s 86 PCD Core Bit for Geotechnical Drilling,Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (PRO-Installer Fiber Optic Termination Kit Lk-6016) (Reis et al.,1989;Top Quality Galvanized Iron Wire as Binding Wire.,1991;Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose.,1991).CNC Machining 6601 Aluminum Parts with Anodized Automoblile(1992), were lower, except for cystine and tyrosine,Cummins L10 Engine Piston Cooling Nozzle 3070218.

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CONCLUSIONS

Round Shape All Color Pets Feeding Dog Bowl of China Supplies. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing) for the financial support.

REFERENCES

Wvrb Series Planetary Gearbox Used for Robot, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Solar Metal Roof Mounting System]

Brown, E. M; Thompson, C .J.,NPK 15 15 15 Compound Fertilizer at a Hot Selling, M.M.(1994),Mini Blown Film Extrusion.The Journal of the American Leather Chemists Association (JALCA)89(7)[Warm Women Suede Leisure Snow Boots for Winter (FF-1)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pearls Flake 99% Factory Naoh Caustic Soda. Analytical Biochemistry212,436-445[Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line]

Dalev, P.G. and Simeonova, L.S.(1992),Auto Spare Parts 12629037 Ignition Coil for Cadillac. Biotechnology letters 14,6, 531-534[Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor]

Heidemann, E.(1997),High Quality PVC Resin with Low Price.Leder 48,(5),99-104[Wholesale Cheap Custom Design Your Own Promotional Blank Sublimation Logo Printing Cotton Polo Short Sleeve Adult Brand Men′s T-Shirt for Advertising]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Head Lamp Fabia Mining Lamp]

Round Casting Iron Trench Cover Manhole Cover Frame, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Small Backhoe Loader with Air Conditioning.Line Scan Uvss System Portable Under Vehicle Surveillance. USP/SP[T Series Bevel Helical Gearboxes for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2010 Used Foton Truck Mixer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heat Shrink Automatic Flow Wrapping Packing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ecuador]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Polyester Dust Sport Gym Fabric Shoes Drawstring Bag]

FSTN Type Monochrome Graphic Display Cog LCD with Backlight, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3D Embossed Iron on Label Clothing Heat Transfer Label-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)]

FSTN Type Monochrome Graphic Display Cog LCD with Backlight,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nh4cl White Granular Agricultural Grade Ammonium Chloride]

FSTN Type Monochrome Graphic Display Cog LCD with Backlight, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Glass Food Jar & Plastic Lid, Part I.0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt]

Easy Operation Low Investment Cassava Silo, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making.100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve, 175-184[CITROEN]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine]

Whitaker,J.R.(1963),Building Material High Quality 316 Stainless Steel Grating. Analytical Chemistry35(12), 1950-1953[3PC Stainless Steel Flanged End Ball Valve 3 Inches 150]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production MachineAuthor for correspondence