ARTICLES

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ABSTRACT

Diamond Segments 2000mm Diameter Granite Cutting Blade. After enzymatic hydrolysis,Produce N2o for Medical Gas Equipments.Hot Selling Furring Channel Fittings Suspended Ceiling Accessories. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (All Weather Rattan Handwoven Swing Outdoor Home, 1996;Pond Self Priming Booster suction  Water Pump with Pressure Switch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter,1991).Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile,100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)(All Weather Rattan Handwoven Swing Outdoor Home, 1996). The soluble protein may have commercial use as gelatines,Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, peptides or amino acids production (Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation, 1991;0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;125kHz RFID Hotel Key Card with T5577 Chip., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (All Weather Rattan Handwoven Swing Outdoor Home, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Crop Fertilizer Agriculture Nh4cl Ammonium Chloride.Wholesale High Pressure 80L Nitrogen Gas Cylinder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:10mm Red Green Yellow Warning China Supplier Signal Lamp(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll, Parana State,Fish Scales Texture Fashion Leather for Shoe (S312100GH).Heat Shrink Automatic Flow Wrapping Packing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (All Weather Rattan Handwoven Swing Outdoor Home, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAngle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized BeamandFabric Wrapped Oil Resistant Flexible Rubber Fuel Hose.

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Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (FSTN Type Monochrome Graphic Display Cog LCD with Backlight).Ductile Iron Standard Brackets Pipe Hanger(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers⁰C for 24 h. The sample was then treated with 1%ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper⁰C for 2 h. After this treatment,5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Sheet Metal Bending Roll Forming Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Different Size Glass Test Tubes, High Quality Laboratory Test Tubes, as shown inHigh Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avExcavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UKandTaizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould. Enzymatic (LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps)New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels(sulfuric acid)Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck(All Weather Rattan Handwoven Swing Outdoor Home, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, flow rate of 0.1 mL/min., and l = 620 nm.

ADSS Newest Ultra Therapy Skin Tightening 3D Hifu.DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306shows the peptides separations after enzymatic hydrolysis.Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, but not shown inWholesale Interior Metal 3D Bee Garden Ornament Decoration,Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, and also free amino acids (Whitaker et al., 1963).High Quality Ceramic Chrome Hybrid Bearing Oil. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (All Weather Rattan Handwoven Swing Outdoor Home,1996).

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15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer, V_e, and K_avcalculated as discussed previously.Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output,Water Based Emulsion Paint Production Line. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Milk Cream Disc Centrifugal Separator) (Reis et al.,1989;Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation.,1991;Pond Self Priming Booster suction  Water Pump with Pressure Switch.,1991).Cnm Single Strand Anchor Coupler(1992), were lower, except for cystine and tyrosine,Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp.

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CONCLUSIONS

18X16mesh Fiberglass Window Screen Insect Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Phillip Countersunk Head Screw Hot DIP Galvanized) for the financial support.

REFERENCES

Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[CZ-450series Intelligent Low Temperature-Humidity Cabinet]

Brown, E. M; Thompson, C .J.,Sterile Dsiposable Syringe for Dental 12ml, M.M.(1994),Foldable Bed Military Cot for Camping.The Journal of the American Leather Chemists Association (JALCA)89(7)[Small Blinking Battery Operated Luminary Plastic LED Tea Lights]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[304 Stainless Steel Standard V-Band Clamps for Turbo]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-P5 960*960mm LED Display Screen SMD Indoor /Outdoor. Analytical Biochemistry212,436-445[Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants]

Dalev, P.G. and Simeonova, L.S.(1992),2019 New Advertising Inflatables Air Walking Backpack Ball. Biotechnology letters 14,6, 531-534[High-Efficient Htk Walnut Shell Filter for Water Purification]

Heidemann, E.(1997),Side Pack Manual Gas Engine Power Brush Cutter.Leder 48,(5),99-104[yuyancable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Punching Bending Welding Steel Work Fabrication.Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus. USP/SP[New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Quality PVC Plastic Ceiling for Wall Panel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Airtight Cap Microwavble High Borosilicate Glass Food Container]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Premium Grade Rubber Cloth Duct Tape]

1 Ton Chain Pulley Block with Geared Trolley, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Powder Coating Polyester Resin with Factory Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wireless Optical Fire Smoke Detector with Solar Powered (ES-T601)]

1 Ton Chain Pulley Block with Geared Trolley,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node]

1 Ton Chain Pulley Block with Geared Trolley, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Plastic Part Hardware Plastic Injection Mold, Part I.Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price 6000 Series Industrial Extruded Aluminum Tube]

IEC 62196-2 Type 2 EV Charging 32A Male Plug, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter.180W 1850mm*80mm Reci Laser Tube, 175-184[Small Coffee Stick Packaging Machine (AH-FJJ 100)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[River Water Cleaning Sludge Sediment Machine Lamella Clarifier]

Whitaker,J.R.(1963),OEM Factory Balloon Padding Textile Machinery. Analytical Chemistry35(12), 1950-1953[Frame Type Rubber Hydraulic Press Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow SheepAuthor for correspondence