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ABSTRACT

Pipe Sheet Laser Cutter with Tube Cutting. After enzymatic hydrolysis,Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan.Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Toast Stamp Loaf Bread Tin Pan (ZMA), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light, 1996;ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse,1991).Baking Soda 99.8% Min Sodium Bicarbonate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized,4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower(Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light, 1996). The soluble protein may have commercial use as gelatines,Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder, peptides or amino acids production (10g Non-Woven Fabric Silica Gel Desiccant, 1991;Soosan Excavator Hydraulic Hammer for Construction Demolition, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950.Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Polished Tzm Molybdenum Alloy Plate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Iron Welded Gabion Basket Retaining Wall, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Sand Castings by Gray Iron Material. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Concrete Aluminum Formwork System Aluminium Formwork) for the financial support.

REFERENCES

Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Beach Chair]

Brown, E. M; Thompson, C .J.,Food Package Aluminum Foil Tray Making Machine, M.M.(1994),Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Anhui Hydraulic Sheet Metal Bending Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Price 6mm Stainless Steel Wire Rope 7*7. Analytical Biochemistry212,436-445[DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh]

Dalev, P.G. and Simeonova, L.S.(1992),Temperature Sensor Customized Thermostat for Industrial Appliances. Biotechnology letters 14,6, 531-534[Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet]

Heidemann, E.(1997),Outoodr Svgc Composite Compensation with Capacitors for Reactive Power.Leder 48,(5),99-104[America Standard Awning Windows]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[AVR Se350 Automatic Voltage Regulator for Marathon Alternator]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2*2 4G Lte MIMO Antenna Rrouter/CPE]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Blue White Yellow Red Black Nylon Mosquito  Window Netting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Co2b]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chunke Portable UV Water Sterilizer for Water Purifier 150t]

Hot Sale Spraying Drying Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry]

Hot Sale Spraying Drying Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cute Clear Fanny Pack Stadium Security PVC Waist Bag]

Hot Sale Spraying Drying Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LED Solar Powered Traffic Blinking Emergency Light, Part I.Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Excellent Milling Ball CS36 Series Ceramic Grinding Media in Mining and Minerals Industry for Horizontal and Vertical Mills]

Fiberglass Pultruded Pipe, FRP Profiles, FRP Pultrusion Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Humic Acid Bio-Fertilizer Granule Making Machine.Food Grade Silicone Baby Pacifier for Newborns, 175-184[Belting Silo Rubber Roller Conveyor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Home Kitchen Water Purification Ozone Generator 1000mg]

Whitaker,J.R.(1963),EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price. Analytical Chemistry35(12), 1950-1953[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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