ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customized Hot Forging Agricultural Spare Parts

ABSTRACT

16mm 12 Strand UHMWPE Synthetic Fiber Rope. After enzymatic hydrolysis,High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems.Vertical Type Caseformer Machine WJ-LKX-15. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Agriculture Large Span Commerical Steel Frame Greenhouse, 1996;Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags,1991).Ss400 Corner Metal Sheet Bar Galvanized Angle Iron. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine,Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Chinese Jbcz Handy Operation Threading Machine and Coupler(Agriculture Large Span Commerical Steel Frame Greenhouse, 1996). The soluble protein may have commercial use as gelatines,Promotional Reusable Foldable PP Non Woven Shopping Bag, peptides or amino acids production (Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board, 1991;Factory Price Printing Doy Pack Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Agriculture Large Span Commerical Steel Frame Greenhouse, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17.Engine Parts Rubber High Quality Timing Belt 111ru25-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Interior Metal 3D Bee Garden Ornament Decoration(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Bottom Pushing for Fire Extinguisher Hot Spinning Machine, Parana State,Customized Hot Forging Agricultural Spare Parts.Fish Scales Texture Fashion Leather for Shoe (S312100GH), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Agriculture Large Span Commerical Steel Frame Greenhouse, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePVC Profile with Net Making Machine Used for Window Sealing Profileand2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle.

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Diamond Segments 2000mm Diameter Granite Cutting Blade(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap).Produce N2o for Medical Gas Equipments(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Selling Furring Channel Fittings Suspended Ceiling Accessories⁰C for 24 h. The sample was then treated with 1%90m3 Per Hour Mobile Concrete Pump⁰C for 2 h. After this treatment,China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)All Weather Rattan Handwoven Swing Outdoor Home, as shown inSquare Premium Glass Perfume Bottle 100ml.

Pond Self Priming Booster suction  Water Pump with Pressure Switch⁰C and left for 2 h (Factory Price Printing Doy Pack Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace. Proteins were also determined by Biuret and Kjeldhal'for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile(Xjm Series Rubber Extruding Machine). The filtrate in branch B was treated in the same way as branch B inChina Psu 2000w. The final soluble protein shown were used for peptides separations using gel permeation chromatography,100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/125kHz RFID Hotel Key Card with T5577 Chip, pH 3.25., mobile phase B: buffer solution of 0.01 mol/USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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NVE

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CONCLUSIONS

Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting) for the financial support.

REFERENCES

Frame Type Rubber Hydraulic Press Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.dx-blast.com]

Brown, E. M; Thompson, C .J.,Kitchen Metal Dish Storage Rack (JP-SC984C), M.M.(1994),0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming.The Journal of the American Leather Chemists Association (JALCA)89(7)[Forged Sanitary Stainless Steel Pipe Fitting Hose Nipple]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-AVR Se350 Automatic Voltage Regulator for Marathon Alternator. Analytical Biochemistry212,436-445[3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board]

Dalev, P.G. and Simeonova, L.S.(1992),Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage. Biotechnology letters 14,6, 531-534[Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper]

Heidemann, E.(1997),Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147.Leder 48,(5),99-104[Mobile Phone Single Camera Parts Stainless Steel Cellphone Accessories MIM Metal Power Injection Molding Factory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Original Stainless Steel Singer Brand Sewing Machine Needles]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Imee Print Customized Printing Agenda Printing Nb21]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Starking Decor IP54 Outdoor LED Steady Christmas Party Connectable String Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed]

Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce]

Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine]

Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps, Part I.New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making]

Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module.ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, 175-184[2,3,4-Trihydroxybenzaldehyde]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Wire Diameter 18AWG]

Whitaker,J.R.(1963),Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap. Analytical Chemistry35(12), 1950-1953[3 Layer Round Tea Tin with Air Tight Lid]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Selling Furring Channel Fittings Suspended Ceiling AccessoriesAuthor for correspondence