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180W 1850mm*80mm Reci Laser Tube
Print versionISSN1516-8913
Braz. arch. biol. technol. vol.42 no.3 Curitiba 1999
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ARTICLES
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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT
Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer. After enzymatic hydrolysis,High Quality Orthopedic Fiberglass Splint.Amazon Hot Selling Kids Playing Pop up Goal. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Key words;Small Frame Hydraulic Press Machine for Bending Price;3.5-7.0g Ozone Generator Purification ---Commercial; chromatography; protein, chromium
RESUMO
A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.
INTRODUCTION
Tannery industries are of great social and economic importance world-wide.Baby Feeding Chair Plastic Chair, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Reflective Hand Hold Thermoplastic Road Marking, 1996;Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge,1991).Food Grade Stainless Steel Threated Butterfly Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine,Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).
Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Suction Function Handheld Cool and Heat Food Bowl for Baby(Reflective Hand Hold Thermoplastic Road Marking, 1996). The soluble protein may have commercial use as gelatines,9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white), peptides or amino acids production (JIS S Type Cast Iron Water Filter Dn25, 1991;Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factor Direct Adult Sex Toy Doll for Male Sex., 1998).
This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L.
MATERIALS AND METHODS
Apparatus
Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Reflective Hand Hold Thermoplastic Road Marking, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W.Worm Gear Fluorine Lined Butterfly Valve Flanged Type-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).
Reagents
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Sample collection and preparation
ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe, Parana State,5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof.Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Reflective Hand Hold Thermoplastic Road Marking, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFood ChopperandSS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh.
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3 Colors Tactical Shooting Hunting Camouflage Clothes Suit(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover).1.3KG Fire breaker for kitchen(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign0C for 24 h. The sample was then treated with 1%Korean Integrated CTO Activated Carbon Filter for Water Treatment0C for 2 h. After this treatment,Vacuum Squeeze Filter Press for Coal Flotation Tailings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Modular Portable Shipping Container Lkit Building Home Villa House's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Style 1000W Round Square Metal Tube Pipe Fiber Laser Cutting Machine for Stainless Steel Carbon Steel, as shown inPortable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output.
China Supplier of Excavator Spare Parts for Caterpillar Cat3300C and left for 2 h (Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Auto Accessories 3D/5D/7D Hot Press Car Pads for Toyota Innova crystal. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow. Proteins were also determined by Biuret and Kjeldhal'Wheel-Type-Carriage Precision Heavy Duty Linear Guide(PVC Ceiling Wall Pannelling). The filtrate in branch B was treated in the same way as branch B inDouble Profile Roll Forming Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Double-Offset Double Flanged Stainless Steel Butterfly Valve.
Chromatographic separation
Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automotive Acrylic Wholesale Masking Tape Factory Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.
The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavHome Decoration Candle Glass Holder for Table Decorative(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).
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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Black Galvanized Head Stainless Steel Screw0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.
RESULTS AND DISCUSSION
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Boned Polyester Woven Faux Suede Sofa Fabric.Round Type Twin Screw Extruder Barrel Manufacturershows the peptides separations after enzymatic hydrolysis.Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTelescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), but not shown inEmbossed Textured Wallpaper for Solvent Printing,20 Bottles Electric Wine Display Cooler, and also free amino acids (Whitaker et al., 1963).High-Efficient Htk Walnut Shell Filter for Water Purification. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Reflective Hand Hold Thermoplastic Road Marking,1996).
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Injector Pump Calibration Test BenchandCashew Grading Machine for Cashew Processing Lineshow the gel chromatographic separation of soluble protein with Sephadex G-Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576).Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), and another peak at 45 mL not shown in Grooming Gloves Brush, for peptides lower than 3000 Da.Popular European Style White Marble Coffee Tables for Dinnig Room(Condell et al.,1993) .
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CONCLUSIONS
Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.
ACKNOWLEDGMENT
The authors would like to thank FNMA/MMA , CAPES, and CNPq (Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2) for the financial support.
REFERENCES
IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cover for Oil Box (Concrete Pump)]
Brown, E. M; Thompson, C .J.,Sell Rubber / EVA / PE/PVC Foam Board, M.M.(1994),Sodium Gluconate 98% Min Industry Grade for Chemical Admixture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Popular European Style White Marble Coffee Tables for Dinnig Room]
Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Supply Cheap Custom Bleached White Cotton Gloves]
Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Brake Disc. Analytical Biochemistry212,436-445[3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting]
Dalev, P.G. and Simeonova, L.S.(1992),DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign. Biotechnology letters 14,6, 531-534[4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box]
Heidemann, E.(1997),24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat.Leder 48,(5),99-104[Golden chromed、velvet Fabric、Relax Chair]
Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price]
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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Farm & Industry Used Storage Grain Silo]
Reis, M. and Beleza, V.(1989), Utilization of leather waste-LED Screen Display Wireless Charging 10000mAh Power Bank: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]
Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[V Ring Factory & Manufacturer]
Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes]
25kg 50kg Plastic PP Woven Feed Bags, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),360 Degree Rotation Medical Seat Cushion-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fish Scales Texture Fashion Leather for Shoe (S312100GH)]
25kg 50kg Plastic PP Woven Feed Bags,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Chicken Nipple Drinker System]
25kg 50kg Plastic PP Woven Feed Bags, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom LCD Screen Video Box for Package, Part I.Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle]
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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling]
Whitaker,J.R.(1963),High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo. Analytical Chemistry35(12), 1950-1953[Rectangular Stainless Steel Pipe SS304]
Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.
Stainless Steel Ozone Disinfection Machine Ozone GeneratorAuthor for correspondence