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ABSTRACT

Transparent Color Stretch Wrap Film Manual Operate Wrap Film. After enzymatic hydrolysis,Good Price Welding Electrode (e7018/e7018-1).Ep/Nn/EPP Industrial Rubber Conveyor Belt. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing;New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Auto Spare Parts Ignition Coil Fp85-18-100c, 1996;vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flooded Type Water Cooled Screw Chiller,1991).Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Enameled Mini Cast Iron Casserole,Good Quality Tomato Sauce Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vitamin B1 HCl 98% Feed Grade(Auto Spare Parts Ignition Coil Fp85-18-100c, 1996). The soluble protein may have commercial use as gelatines,2018 TPR Clean Teeth Training Dog Pet Chew Toy, peptides or amino acids production (Good Quality Zinc Plated Slotted Hex Head Roofing Screw, 1991;Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate Superplasticizer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Orthopedic Fiberglass Splint. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Auto Spare Parts Ignition Coil Fp85-18-100c, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Amazon Hot Selling Kids Playing Pop up Goal.Small Frame Hydraulic Press Machine for Bending Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3.5-7.0g Ozone Generator Purification ---Commercial(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity).Suction Function Handheld Cool and Heat Food Bowl for Baby(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white)⁰C for 24 h. The sample was then treated with 1%JIS S Type Cast Iron Water Filter Dn25⁰C for 2 h. After this treatment,Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factor Direct Adult Sex Toy Doll for Male Sex's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L, as shown inAuto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLuxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3 Colors Tactical Shooting Hunting Camouflage Clothes Suit, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Home Decoration Candle Glass Holder for Table Decorative, V_e, and K_avcalculated as discussed previously.Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars,Wholesale Black Galvanized Head Stainless Steel Screw. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (German Technology Automatic Stacking System in Red Brick Automatic Production Line) (Reis et al.,1989;Good Quality Zinc Plated Slotted Hex Head Roofing Screw.,1991;vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment.,1991).Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle(1992), were lower, except for cystine and tyrosine,Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair.

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CONCLUSIONS

OEM Logo Soft Silicone Reusable BPA Free Drinking Straws. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)) for the financial support.

REFERENCES

Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Fabricated High Quality Steel Structure Warehouse]

Brown, E. M; Thompson, C .J.,4 Drawer Steel Office Vertical Filing Cabinet, M.M.(1994),12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Parts for Crane Outrigger Hydraulic Oil Cylinder. Analytical Biochemistry212,436-445[All Weather Rattan Handwoven Swing Outdoor Home]

Dalev, P.G. and Simeonova, L.S.(1992),Boned Polyester Woven Faux Suede Sofa Fabric. Biotechnology letters 14,6, 531-534[Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Wholesale Qualited Polyester Padding Men Jacket]

Manual Vertical Car Key Cutting Machine for Locksmith, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),20 Bottles Electric Wine Display Cooler.High-Efficient Htk Walnut Shell Filter for Water Purification. USP/SP[Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Clay Tiles Making Machine with Latest Technology]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cardboard Game With Chips Factory & Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[I-Tech 12000HD 2u Space Digital Professional Power Amplifier]

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Popular European Style White Marble Coffee Tables for Dinnig Room-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PTFE Seat Wafer Butterfly Valve Pn16]

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore]

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket, Part I.FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Selling Military Large Event Marquee Tent Sale]

Kwikstage Scaffolding System-Diagonal Brace, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),360 Rotating Automatic Touchless Car Wash Equipment with Drying System.48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, 175-184[m.rfidfs.net]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall]

Whitaker,J.R.(1963),Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox. Analytical Chemistry35(12), 1950-1953[Wholesale Custom Football Net Soccer Goal Net for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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