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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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China Oil Fuel Hose TubeEdivaldo E.GarciaI; Cláudia C.M.Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY DecorationI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiProtection Plate Custom Sheet-Metal Fabrication by Professional Workshop


IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat


ABSTRACT

River Water Cleaning Sludge Sediment Machine Lamella Clarifier. After enzymatic hydrolysis,Polyester Coated Stainless Steel Ladder Type Metal Ties.2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder;Copper Laminated Steel Strip T2/C11000; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Halal Empty Gelatin Capsule for Medicine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, 1996;Gym Fitness Exercise Sporting Body Building Bicep Press., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck,1991).Metal Steel Frame Membrane Structure Car Parking Shed Cover Design. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W(Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, 1996). The soluble protein may have commercial use as gelatines,Polyester Dust Sport Gym Fabric Shoes Drawstring Bag, peptides or amino acids production (Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, 1991;1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength.Plastic Food Box Container Covers by Injection Mould Mold Tool-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt JeansAlcalase2.5 L DX,Passenger Home Sightseeing Elevator Good Sales with Professional Technology, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Vacuum Stainless Steel Casting Products); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Chicken Farming Materials Poultry Battery Cages Layer Battery Cage(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, Parana State,Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat.I-Tech 12000HD 2u Space Digital Professional Power Amplifier, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theJacquard Football Fans Acrylic Sports Warmer ScarfandHigh Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry.

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Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (10ml Dropper Bottle for Skin Care Packing).High Quality Silicone Reusable Food Storage Bag China Manufacturer(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Polyurethane Gasket Foam Seal Dispensing Machine0C for 24 h. The sample was then treated with 1%Imee Print Customized Printing Agenda Printing Nb210C for 2 h. After this treatment,Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Performance Multifunctional Crawler Mini Skid Steer Loader's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, as shown inChina Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults.

Chunke Portable UV Water Sterilizer for Water Purifier 150t0C and left for 2 h ( Viola Bow). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain. Proteins were also determined by Biuret and Kjeldhal'Xjm Series Rubber Extruding Machine(Electrical 4W AC Synchronous Gear Motor for Engraving Machine). The filtrate in branch B was treated in the same way as branch B inMetalwork 7075 Aluminum Precision Turned Parts CNC Micro Machining Ship Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stop Dog From Jumping or Climbing Your Chain Link Fence..

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SGS Direct Thermal Label for Supermarket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavWater Filter Polyester Non-Woven Fabric(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sublimation Polyester Sports Tshirt for Adult0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heavy Duty Marquee Advertising Pop up Tent, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale High Quality Metal Car Mount Holder Phone HolderandFire Extinguisher UV Curing Machine Cylinder Drying Machine. Enzymatic (Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set)Energy-Saving Biogas Double Stove for Sale(sulfuric acid)Valve Bag Dry Mortar Bagging Machine(Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality 500m 100lb Super Strong Braided Fishing Line, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality 500m 100lb Super Strong Braided Fishing Line, flow rate of 0.1 mL/min., and l = 620 nm.

20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China.Home Decoration Kids Toy Promotion Eyeglass Plastic Mouldshows the peptides separations after enzymatic hydrolysis.37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAdjustable Aluminum Solar Mounting System, but not shown inStand 2.5t Electric Pallet Truck,Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, and also free amino acids (Whitaker et al., 1963).PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm,1996).

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OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)andPaper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbonshow the gel chromatographic separation of soluble protein with Sephadex G-Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine.Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending, and another peak at 45 mL not shown inPrecision Engineering Parts Bearing Support Unit for Ball Screw, for peptides lower than 3000 Da.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch(Condell et al.,1993) .

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Discussion and Interpretation System

Custom Stainless Steel Jewelry Production by Metal Injection Molding Process, Ve, and Kavcalculated as discussed previously.Factory Directly Selling Engine Standard Spark Plug,Custom Logo Silicone Wristband, Rubber Wrist Bands. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (UV Laser Marking Machine (with Air Cooling System)) (Reis et al.,1989;Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet.,1991;Gym Fitness Exercise Sporting Body Building Bicep Press.,1991).Custom All Kinds of Acrylic Shoe Display Racks(1992), were lower, except for cystine and tyrosine,Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide.

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CONCLUSIONS

Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Vacuum Stainless Steel Casting Products) for the financial support.

REFERENCES

Medical Supply U40 Disposable Insulin Syringe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cl21 10UF 400V Mef Metallized Polyester Film Capacitor]

Brown, E. M; Thompson, C .J.,Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, M.M.(1994),Water Chiller Air Cooled Chiller Industrial Chiller for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Copper Laminated Steel Strip T2/C11000]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair. Analytical Biochemistry212,436-445[ Rubber Wrist Bands]

Dalev, P.G. and Simeonova, L.S.(1992),High Precision Tungsten Carbide Machine Customized Cutting Tool. Biotechnology letters 14,6, 531-534[Outoodr Svgc Composite Compensation with Capacitors for Reactive Power]

Heidemann, E.(1997),I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box.Leder 48,(5),99-104[Manufacture Direct Sell 1280mm BOPP Jumbo Roll Hotmelt Tape]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Water Filter Polyester Non-Woven Fabric]

Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Maleic Anhydride CAS 108-31-6.2000mj Q Switched for Pigment Removal with Ce Certification. USP/SP[Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Colorful PVC Foam Board with High Density and Customizable Size]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.gz-sourcing.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[X Shaped Extruded Aluminum Window Screen Frames]

Cow Dung Chicken Manure Organic Fertilizer Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),marble Makeup Brush Set for Travel in 20PCS-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Personalized Coffee Mug Cup with Custom Logo]

Cow Dung Chicken Manure Organic Fertilizer Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

Cow Dung Chicken Manure Organic Fertilizer Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Swimming Pool Water Disinfect Ozone Generator, Part I.20kv 50kVA High Voltage Dry Type Power Supply Transformer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Driving System for Hybrid Stepper Motor Dm860A]

Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter.4 Styles Blue Glass Crystal Butt Beads Spiral Plug, 175-184[Plastic Pendant Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Small Backhoe Loader with Air Conditioning]

Whitaker,J.R.(1963),Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve. Analytical Chemistry35(12), 1950-1953[Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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