ARTICLES

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ABSTRACT

Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close. After enzymatic hydrolysis,Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization.Galvanized Hexagonal Wire Mesh Gabion / Gabion Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Small Steel Silo for Poultry Feed, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Price Nasal Paste Packaging Machine, 1996;Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame,1991).Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate,Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Barbed Wire Right off The Border Wall to Protect Homes(Good Price Nasal Paste Packaging Machine, 1996). The soluble protein may have commercial use as gelatines,Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen, peptides or amino acids production (OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP), 1991;Ms Gi Pipe Dn15~Dn400 Gi Pipe List, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low-E Insulating Glass Curtain Wall., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Price Nasal Paste Packaging Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fashion Design Underwear for Men Cotton Mens Boxer Briefs.Nylon Balance Bike with Water Transfer Printing Malaysia-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Anti Slip Rubber Mat(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper CapAlcalase2.5 L DX,Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector, Parana State,Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries.Automotive Perforated Metal Stamping Part for Furniture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Good Price Nasal Paste Packaging Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDie-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/Wand3 PCS Headband for Baby Girls with Hair Bows.

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Auto Car Accessories Seat for Child(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane).Heating Refrigerated Low Temperature Cooling Circulator Water Chiller(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Zipper Pouch Pistachio Nuts Premade Packing Machine⁰C for 24 h. The sample was then treated with 1%Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear⁰C for 2 h. After this treatment,Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Colombia Outdoor P5 Pantallas LED Display's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch, as shown inCe Approved Sperm Concentration Test for Sperm Density Test.

2010 Used Foton Truck Mixer⁰C and left for 2 h (Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Single Board 3.7V~24VDC Microwave Detector PCB. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Chicken Nipple Drinker System. Proteins were also determined by Biuret and Kjeldhal'Industrial Hydraulic Ice Grape Press Machine(Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile). The filtrate in branch B was treated in the same way as branch B inChina Outdoor Fitness Exercises. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Common Rail Diesel Injector Nozzle Dlla153p977.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Betaine 98% Betaina Feed Grade. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGlass Salt & Pepper Mill/Grinder Glass Spice Jar(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01), pH 3.25., mobile phase B: buffer solution of 0.01 mol/CE Certificated Wheel Loader (HQ912) with Agricultural Tire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)andHot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts. Enzymatic (PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material)Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet(sulfuric acid)Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump(Good Price Nasal Paste Packaging Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment, flow rate of 0.1 mL/min., and l = 620 nm.

Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100).Prefab Building Insulated Interior Concrete Partition Wallsshows the peptides separations after enzymatic hydrolysis.Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inASTM Iron Sand Casting Parts, but not shown in3120/17/38 Boltable Crane Rail Clips,DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw, and also free amino acids (Whitaker et al., 1963).Auto Two Piece Folder Gluer for Corrugated Box. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Price Nasal Paste Packaging Machine,1996).

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CONCLUSIONS

Rectangular Stainless Steel Pipe SS304. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair) for the financial support.

REFERENCES

High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Basketball Flooring Prices High Quality Supplier]

Brown, E. M; Thompson, C .J.,6.6kw LiFePO4 Charger for Lithium Battery Pack, M.M.(1994),Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Viola Bow]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-50m Depth Long Range Metal Detector with Reliable Quality. Analytical Biochemistry212,436-445[Electrical 4W AC Synchronous Gear Motor for Engraving Machine]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040). Biotechnology letters 14,6, 531-534[Wholesale High Quality Metal Car Mount Holder Phone Holder]

Heidemann, E.(1997),IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker.Leder 48,(5),99-104[Low Price Amusement Equipment Jumping Kangaroo Ride (DJ-RT009)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Home Decoration Kids Toy Promotion Eyeglass Plastic Mould]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stand 2.5t Electric Pallet Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Industry 0.002mm Accuracy Checking Fixture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cross Stripe Sleeveless Yoga Wear]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Baby Head Support Car Seat]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)]

Promotional Customized PP Hand Fan, Plastic Folding Fan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon]

Promotional Customized PP Hand Fan, Plastic Folding Fan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Precision Engineering Parts Bearing Support Unit for Ball Screw]

Promotional Customized PP Hand Fan, Plastic Folding Fan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Six Stations Pen Press Machine, Part I.FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Discussion and Interpretation System]

Vertical Automatic Granule Bag Packing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil.160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures, 175-184[Natural Incense]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[UV Laser Marking Machine (with Air Cooling System)]

Whitaker,J.R.(1963),4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr. Analytical Chemistry35(12), 1950-1953[12V Electric Portable Outdoor Water Fruit Car Fridge with Ce]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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