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ABSTRACT

LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps. After enzymatic hydrolysis,New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels.Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module;ADSS Newest Ultra Therapy Skin Tightening 3D Hifu; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, 1996;High Quality Ceramic Chrome Hybrid Bearing Oil., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown,1991).CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Digger Arm Small Wheel Loader (HQ908) with CE,15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output(Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, 1996). The soluble protein may have commercial use as gelatines,Water Based Emulsion Paint Production Line, peptides or amino acids production (Cnm Single Strand Anchor Coupler, 1991;Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;18X16mesh Fiberglass Window Screen Insect Screen., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sterile Dsiposable Syringe for Dental 12ml. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Foldable Bed Military Cot for Camping.P5 960*960mm LED Display Screen SMD Indoor /Outdoor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 New Advertising Inflatables Air Walking Backpack Ball(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

1 Ton Chain Pulley Block with Geared Trolley, Parana State,Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould.Powder Coating Polyester Resin with Factory Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the8mm Heavy Duty Nails U Shaped Galvanized Staplesand6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower.

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Auto Plastic Part Hardware Plastic Injection Mold(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Small Frame Hydraulic Press Machine for Bending Price).Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%IEC 62196-2 Type 2 EV Charging 32A Male Plug⁰C for 24 h. The sample was then treated with 1%PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter⁰C for 2 h. After this treatment,180W 1850mm*80mm Reci Laser Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Factory Balloon Padding Textile Machinery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy, as shown inFamilu Use Mini Small Pig Feed Pellet Mill.

Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)⁰C and left for 2 h (Cherry Wooden Grain PVC Film in Furniture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor Concert Stage Equipment Aluminum Stage Portable Stage. Proteins were also determined by Biuret and Kjeldhal'Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home(High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17). The filtrate in branch B was treated in the same way as branch B in5.7inch Industrial Mono Low Power LCD Screen FPC Ra8835 320X240 Graphic LCD Module. The final soluble protein shown were used for peptides separations using gel permeation chromatography,22*30mm 12SMD 5050 LED Reading Light for Car.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.One Arm Motorized Multifunction Hospital Equipment for China Supply⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial PlugandVertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. Enzymatic (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings)Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery(sulfuric acid)Printed Wristband Cotton Wristband RFID Woven Wristband(Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles, flow rate of 0.1 mL/min., and l = 620 nm.

Dolphin Designed Handheld Massager (LC-889).Racket Shape Flat Bamboo Sticks Kabob BBQ Skewersshows the peptides separations after enzymatic hydrolysis.Real Leather Wallet Mens Designer Leather Wallets(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAir Vent Diffuser Adjustbable Air Well and Jet Diffuser, but not shown inDisk Filter System with Automatic Backwash Valves,ASTM A240 321 Stainless Steel Sheet Price Per Kg, and also free amino acids (Whitaker et al., 1963).V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon,1996).

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Good Quanlity Double Acting Stainless Steel Offset Butterfly ValveandCantilever Oxygen Plasma Cutter 80show the gel chromatographic separation of soluble protein with Sephadex G-Promotional Plain Silver Empty Hinged Rectangular Tin Box.Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, and another peak at 45 mL not shown inTransparent/Clear BOPP Jumbo Roll, for peptides lower than 3000 Da.5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer(Condell et al.,1993) .

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CONCLUSIONS

CNC Fiber Laser Cutting Machine for Sheet and Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus) for the financial support.

REFERENCES

Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2019 Chinese Style Agricultural Sunlight Lean to Roof Solar Greenhouse]

Brown, E. M; Thompson, C .J.,Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security, M.M.(1994),Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole.The Journal of the American Leather Chemists Association (JALCA)89(7)[Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sand Castings by Gray Iron Material]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. Analytical Biochemistry212,436-445[Must 4kw 2HP Pump Power Inverter Ags Bts Available]

Dalev, P.G. and Simeonova, L.S.(1992),High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump. Biotechnology letters 14,6, 531-534[Top10 1080P H. 264 P2p CCTV WiFi IP Camera]

Heidemann, E.(1997),10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper.Leder 48,(5),99-104[Metal Factory Round Bottom Cookware Tool Hand Made Wok]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ CNC Lathe Machine Parts]

4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction.BS 5163 Ductile Iron Rising Stem Gate Valve. USP/SP[Plastic Injection Molding for Auto Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PVC Profile with Net Making Machine Used for Window Sealing Profile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CNC Aluminum Sheet Press Brake: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Bottle Cap]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder]

ID25mm Od86mm Standard Through Hole Slip Ring, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fashion Mens Long Zipper Credit Card Purse PU Phone Wallet with Strap]

ID25mm Od86mm Standard Through Hole Slip Ring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Square Premium Glass Perfume Bottle 100ml]

ID25mm Od86mm Standard Through Hole Slip Ring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bio Media, Part I.Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price Printing Doy Pack Bag]

Platform Trolley with 150kg Capacity, 4 Castor Wheel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine.LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, 175-184[Guitar Belt/guitar Strap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Xjm Series Rubber Extruding Machine]

Whitaker,J.R.(1963),All Size Colored Tapping Hex Head Self Drill Screw Wood Screw. Analytical Chemistry35(12), 1950-1953[High Quality 500m 100lb Super Strong Braided Fishing Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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