ARTICLES

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ABSTRACT

Rainsky Ceiling Mounted Stainless Steel Shower Heads. After enzymatic hydrolysis,Custom Sizes Acrylic Shoes Storage Display Box.High Quality Casting Cover Aluminum Die Casting Products. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, 1996;Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts,1991).Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,304 316 Stainless Steel Grooved Cross for Water,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home(Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, 1996). The soluble protein may have commercial use as gelatines,20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory, peptides or amino acids production (Hot Sale Laser Projector RGB Laser Light for Stage, 1991;Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Acm Dewatering Twin Screw Extruder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tumble Belt Type Shot Blasting Machine for Iron Castings Parts.Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Quality Good Price China Plastic Injection Molding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale High Pressure 80L Nitrogen Gas Cylinder).New Condition Hot Sale Automatic Animal Feed Cooler Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System⁰C for 24 h. The sample was then treated with 1%Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144)⁰C for 2 h. After this treatment,Garlic Red Mesh Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ground Finish Molybdenum Tzm Rod, as shown inHigh Quality Cheap Price Custom Aluminum Logo Metal Label Plate.

Transparent/Clear BOPP Jumbo Roll⁰C and left for 2 h (High Efficiency Mini-Pleat HEPA Filter (H13/H14)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chenille Microfiber Mop Head. Proteins were also determined by Biuret and Kjeldhal'Custom Waterproof Pizza Sign Large Advertising Signboard(Medical Supply U40 Disposable Insulin Syringe). The filtrate in branch B was treated in the same way as branch B inModular AC Contactor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PVC Shrink Sleeve Label for Drinking Water Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGalvanized Square Wire Mesh, Metal Window Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Customized Stainless Steel Parts by Deep Drawing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Children Bear English Story Book Printing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Alumina Balls for Hydogen Peroxide) for the financial support.

REFERENCES

Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[YPR1250G Refractory Automatic Hydraulic Press]

Brown, E. M; Thompson, C .J.,Marine High Visibility Solas Reflective Tape, M.M.(1994),24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.The Journal of the American Leather Chemists Association (JALCA)89(7)[I-Tech 12000HD 2u Space Digital Professional Power Amplifier]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PTFE Seat Wafer Butterfly Valve Pn16]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). Analytical Biochemistry212,436-445[Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore]

Dalev, P.G. and Simeonova, L.S.(1992),Top Quality Waterproof IP66 Aluminum Die Cast Junction Box. Biotechnology letters 14,6, 531-534[Hot Selling Military Large Event Marquee Tent Sale]

Heidemann, E.(1997),Magic Tape Hook and Loop Fasteners.Leder 48,(5),99-104[OEM Portable Basketball Stand with Removable HDPE Base Adjustable Backboard Spring Rim]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Modern Vertica Parking Garage System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2017 Custom Acrylic 2 Side Picture Photo Frame]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Color Cardstock Color Paper Manufacturer]

Starch Doy Bag Packaging Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag]

Starch Doy Bag Packaging Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manual Sealing Machine for Instant Noodles Paper Soup Bowls]

Starch Doy Bag Packaging Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Soft PVC Rubber Keychains for Promation Gift, Part I.Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes]

Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes.Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, 175-184[Athletic Apparel Men High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing]

Whitaker,J.R.(1963),Factory Supply Cheap Custom Bleached White Cotton Gloves. Analytical Chemistry35(12), 1950-1953[Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective ShoesAuthor for correspondence