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Food Grade Stainless Steel Threated Butterfly Valve
Print versionISSN1516-8913
Braz. arch. biol. technol. vol.42 no.3 Curitiba 1999
China Supplier of Excavator Spare Parts for Caterpillar Cat330
ARTICLES
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H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof CameraEdivaldo E.GarciaI; Cláudia C.M.Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed SocksI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge Nozaki2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide
IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Railway Freight Wagon Spare Parts Casting and Forging for Bogie
ABSTRACT
Rainsky Ceiling Mounted Stainless Steel Shower Heads. After enzymatic hydrolysis,Custom Sizes Acrylic Shoes Storage Display Box.High Quality Casting Cover Aluminum Die Casting Products. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Key words;Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags;304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser; chromatography; protein, chromium
RESUMO
A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.
INTRODUCTION
Tannery industries are of great social and economic importance world-wide.SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, 1996;Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts,1991).Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,304 316 Stainless Steel Grooved Cross for Water,Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).
Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home(Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, 1996). The soluble protein may have commercial use as gelatines,20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory, peptides or amino acids production (Hot Sale Laser Projector RGB Laser Light for Stage, 1991;Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer., 1998).
This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Acm Dewatering Twin Screw Extruder.
MATERIALS AND METHODS
Apparatus
Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Tumble Belt Type Shot Blasting Machine for Iron Castings Parts.Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Quality Good Price China Plastic Injection Molding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).
Reagents
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Sample collection and preparation
Hot Selling Electronical Blue Pet Plastic Release Film Price, Parana State,Railway Freight Wagon Spare Parts Casting and Forging for Bogie.DIN127 Stainless Steel Spring Lock Washer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHeavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding BracketandTemperature Sensor Customized Thermostat for Industrial Appliances.
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29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale High Pressure 80L Nitrogen Gas Cylinder).New Condition Hot Sale Automatic Animal Feed Cooler Machine(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System0C for 24 h. The sample was then treated with 1%Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144)0C for 2 h. After this treatment,Garlic Red Mesh Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ground Finish Molybdenum Tzm Rod, as shown inHigh Quality Cheap Price Custom Aluminum Logo Metal Label Plate.
Transparent/Clear BOPP Jumbo Roll0C and left for 2 h (High Efficiency Mini-Pleat HEPA Filter (H13/H14)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Chenille Microfiber Mop Head. Proteins were also determined by Biuret and Kjeldhal'Custom Waterproof Pizza Sign Large Advertising Signboard(Medical Supply U40 Disposable Insulin Syringe). The filtrate in branch B was treated in the same way as branch B inModular AC Contactor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PVC Shrink Sleeve Label for Drinking Water Bottle.
Chromatographic separation
Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.
The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavGalvanized Square Wire Mesh, Metal Window Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).
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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Customized Stainless Steel Parts by Deep Drawing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Children Bear English Story Book Printing, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.
RESULTS AND DISCUSSION
Manhole Galvanized Steel Trench Cover for DrainandFlexible Board FPC PCB Camera Module Rigid Flex PCB. Enzymatic (High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing)Cherry Wooden Grain PVC Film in Furniture(sulfuric acid)Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine(Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV, flow rate of 0.1 mL/min., and l = 620 nm.
Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44).German Technology Automatic Stacking System in Red Brick Automatic Production Lineshows the peptides separations after enzymatic hydrolysis.Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, but not shown inCanton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine,Linen Quiltcover Set with Quilting, and also free amino acids (Whitaker et al., 1963).2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes,1996).
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Bto 22 Electric Galvanized Razor Concertina Wire for Military, Ve, and Kavcalculated as discussed previously.Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights,Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft) (Reis et al.,1989;Hot Sale Laser Projector RGB Laser Light for Stage.,1991;Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut.,1991).Hot Selling Military Large Event Marquee Tent Sale(1992), were lower, except for cystine and tyrosine,High-Quality Pre-Seasoned Cast Iron Wok.
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CONCLUSIONS
2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.
ACKNOWLEDGMENT
The authors would like to thank FNMA/MMA , CAPES, and CNPq (Alumina Balls for Hydogen Peroxide) for the financial support.
REFERENCES
Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[YPR1250G Refractory Automatic Hydraulic Press]
Brown, E. M; Thompson, C .J.,Marine High Visibility Solas Reflective Tape, M.M.(1994),24L Thermoelectric Cooler and Warmer Car Mini Car Fridge.The Journal of the American Leather Chemists Association (JALCA)89(7)[I-Tech 12000HD 2u Space Digital Professional Power Amplifier]
Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PTFE Seat Wafer Butterfly Valve Pn16]
Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444). Analytical Biochemistry212,436-445[Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore]
Dalev, P.G. and Simeonova, L.S.(1992),Top Quality Waterproof IP66 Aluminum Die Cast Junction Box. Biotechnology letters 14,6, 531-534[Hot Selling Military Large Event Marquee Tent Sale]
Heidemann, E.(1997),Magic Tape Hook and Loop Fasteners.Leder 48,(5),99-104[OEM Portable Basketball Stand with Removable HDPE Base Adjustable Backboard Spring Rim]
Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall]
Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool.Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010). USP/SP[Wholesale Custom Football Net Soccer Goal Net for Sale]
O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Modern Vertica Parking Garage System]
Reis, M. and Beleza, V.(1989), Utilization of leather waste-25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration]
Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2017 Custom Acrylic 2 Side Picture Photo Frame]
Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Color Cardstock Color Paper Manufacturer]
Starch Doy Bag Packaging Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag]
Starch Doy Bag Packaging Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manual Sealing Machine for Instant Noodles Paper Soup Bowls]
Starch Doy Bag Packaging Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Soft PVC Rubber Keychains for Promation Gift, Part I.Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes]
Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes.Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, 175-184[Athletic Apparel Men High Quality Supplier]
Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing]
Whitaker,J.R.(1963),Factory Supply Cheap Custom Bleached White Cotton Gloves. Analytical Chemistry35(12), 1950-1953[Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring]
Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.
Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective ShoesAuthor for correspondence