ARTICLES

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ABSTRACT

Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving. After enzymatic hydrolysis,German Technology Automatic Stacking System in Red Brick Automatic Production Line.Top Quality Galvanized Iron Wire as Binding Wire. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, 1996;100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Square Premium Glass Perfume Bottle 100ml,1991).Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed,Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing(Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, 1996). The soluble protein may have commercial use as gelatines,High Quality Inflatable PVC Flocking Health Bed, peptides or amino acids production (Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence, 1991;Cast Iron Butterfly Valve with Handles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Furniture Grade E1 Glue Plywood with Competitive Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Underground Garden 6W High Power Outdoor LED Lawn Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stamping Metal Parts Manufacturer Sheet Parts Fabrication.Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, Parana State,Impact Sound Isolation Wall Clip.Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePet Laminate Aluminium Foil/Pet+Alu+PEandShenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure.

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Stainless Steel Ozone Disinfection Machine Ozone Generator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable).Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PVC Profile with Net Making Machine Used for Window Sealing Profile⁰C for 24 h. The sample was then treated with 1%Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch⁰C for 2 h. After this treatment,Bobby Flay Websites Restaurant Martha Stewart Designer Tray. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, as shown inAluminum Foil Heat Shield Screen Matting Adhesive Backed.

Diameter 2.68m Paragraphed Autoclave for AAC Bricks⁰C and left for 2 h (Clear PVC Sheet with Coating for Card). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Travelling Weekender Mens Canvas Duffle Gym Sports Bag. Proteins were also determined by Biuret and Kjeldhal'4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box(Customized Stable Burn-in Test Equipment). The filtrate in branch B was treated in the same way as branch B inEco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Selling Household Living Room Mat Chenille Carpets. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av130HP 4WD Large Farm Power Engine Tractor From High Quality Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Large Size Outdoor Store Front Signs Advertising Billboard⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1325 CNC Engraving Router, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Manufacturer Wallpaper House Decoration Wall PaperandGarden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel. Enzymatic (T6s 86 PCD Core Bit for Geotechnical Drilling)Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines(sulfuric acid)CNC Machining 6601 Aluminum Parts with Anodized Automoblile(Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cummins L10 Engine Piston Cooling Nozzle 3070218, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cummins L10 Engine Piston Cooling Nozzle 3070218, flow rate of 0.1 mL/min., and l = 620 nm.

Round Shape All Color Pets Feeding Dog Bowl of China Supplies.Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tubeshows the peptides separations after enzymatic hydrolysis.Wvrb Series Planetary Gearbox Used for Robot(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, but not shown in3pH in 3 pH out High Frequency Online UPS - Modular UPS 10 -300kVA,NPK 15 15 15 Compound Fertilizer at a Hot Selling, and also free amino acids (Whitaker et al., 1963).Mini Blown Film Extrusion. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine,1996).

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Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty MachineandCustom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screenshow the gel chromatographic separation of soluble protein with Sephadex G-Pearls Flake 99% Factory Naoh Caustic Soda.Auto Spare Parts 12629037 Ignition Coil for Cadillac, and another peak at 45 mL not shown inNatural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier, for peptides lower than 3000 Da.High Quality PVC Resin with Low Price(Condell et al.,1993) .

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Small Backhoe Loader with Air Conditioning, V_e, and K_avcalculated as discussed previously.Line Scan Uvss System Portable Under Vehicle Surveillance,Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes) (Reis et al.,1989;Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence.,1991;100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap.,1991).FSTN Type Monochrome Graphic Display Cog LCD with Backlight(1992), were lower, except for cystine and tyrosine,3D Embossed Iron on Label Clothing Heat Transfer Label.

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CONCLUSIONS

Glass Food Jar & Plastic Lid. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla) for the financial support.

REFERENCES

0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[6 Inch Pvc Check Valve]

Brown, E. M; Thompson, C .J.,Easy Operation Low Investment Cassava Silo, M.M.(1994),CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making.The Journal of the American Leather Chemists Association (JALCA)89(7)[Promotional Plain Silver Empty Hinged Rectangular Tin Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[24k Gold Whitening Anti-Spot Mask]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve. Analytical Biochemistry212,436-445[Low-E Insulating Glass Curtain Wall]

Dalev, P.G. and Simeonova, L.S.(1992),Building Material High Quality 316 Stainless Steel Grating. Biotechnology letters 14,6, 531-534[20kv 50kVA High Voltage Dry Type Power Supply Transformer]

Heidemann, E.(1997),Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table.Leder 48,(5),99-104[Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Enameled Mini Cast Iron Casserole]

40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sc-2400 Automatic Multi Function Sosn Side Drilling Machine.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. USP/SP[Modular Portable Shipping Container Lkit Building Home Villa House]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manual Vertical Car Key Cutting Machine for Locksmith]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Quality Shaper Machine Bc6085 Shapping Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Cacao Bean Grinder Cocoa Butter Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.hyrubbers.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1650mm*80mm Stable Power Laser Tube]

Thermoplastic Road Marking Paint Glass Beads, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835]

Thermoplastic Road Marking Paint Glass Beads,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hero Brand Polypropylene Bags Machine]

Thermoplastic Road Marking Paint Glass Beads, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, Part I.I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bobby Flay Websites Restaurant Martha Stewart Designer Tray]

Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Free Sample! AC Power Contactor for Sale.PA System Loudspeaker 100W Outdoor Column Speaker , 175-184[Hot Sell Wireless Headphone Bluetooth Headset Hbq I7 Earphone]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Export Grade Citric Acid Monohydrate as Detergent]

Whitaker,J.R.(1963),Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel. Analytical Chemistry35(12), 1950-1953[1325 CNC Engraving Router]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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