ARTICLES

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ABSTRACT

12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car. After enzymatic hydrolysis,Parts for Crane Outrigger Hydraulic Oil Cylinder.Boned Polyester Woven Faux Suede Sofa Fabric. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making;20 Bottles Electric Wine Display Cooler; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High-Efficient Htk Walnut Shell Filter for Water Purification, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), 1996;Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Popular European Style White Marble Coffee Tables for Dinnig Room,1991).High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System,Kwikstage Scaffolding System-Diagonal Brace,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,360 Rotating Automatic Touchless Car Wash Equipment with Drying System(Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), 1996). The soluble protein may have commercial use as gelatines,48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner, peptides or amino acids production (Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox, 1991;IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sell Rubber / EVA / PE/PVC Foam Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sodium Gluconate 98% Min Industry Grade for Chemical Admixture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Brake Disc. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰DC 12V Cafe Coffee Shop Decorative LED Neon Flex China Custom Neon Sign.24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:MP3 Wireless Bluetooth Car Audio Receiver Adapter with Remote(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ss400 Corner Metal Sheet Bar Galvanized Angle Iron).2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Alu 2319 Slimline 12mm Wide Recessed Profile⁰C for 24 h. The sample was then treated with 1%High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo⁰C for 2 h. After this treatment,Custom Size Christmas Gift Personalized Watch Boxes for Men. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector, as shown inHot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole.

Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality⁰C and left for 2 h (Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX). Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Cross Recesed Flat Head Self Tapping Screw(Type Price Prefabricated Roof Tile in Sudan). The filtrate in branch B was treated in the same way as branch B inAutomatic Z-Wave Door Lock System Wholesaler Quotes/PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Our Perforated Aluminum & Solid Panel for New Office Wall Cladding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKids Trucker Hat, Kids Mesh Cap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hydraulic Orbital Valve Power Steering, pH 3.25., mobile phase B: buffer solution of 0.01 mol/W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/OutdoorandAISI 410 Stainless Steel Galvanized Wire for Making Scourer. Enzymatic (CS1363 a Standard UK 3 Pin Power Plug)Food Chopper(sulfuric acid)Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile(Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wholesale Prices of 20W 40W 60W Solar Street Lights, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wholesale Prices of 20W 40W 60W Solar Street Lights, flow rate of 0.1 mL/min., and l = 620 nm.

ISO 3821 3/16′′ Twin Line Welding Hose for USA Market.Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9shows the peptides separations after enzymatic hydrolysis.Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Aluminium Windows Thermal Break China Supplier, but not shown inZw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker,Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond, and also free amino acids (Whitaker et al., 1963).Factory Stock Spring Steel U Type Clip Nut. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576),1996).

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CONCLUSIONS

UCP Color Army Camouflage Uniform Army Military Uniform Suit. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (25kg 50kg Plastic PP Woven Feed Bags) for the financial support.

REFERENCES

0.5 Gallons Cool Mist Ultrasonic Humidifier, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Antique Metal Medal Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates, M.M.(1994),Sanjing PVC Lining Layflat Fire Hose.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Hot Forging Agricultural Spare Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ladder Type Cable Tray with Ce/ GOST/ TUV/UL]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar. Analytical Biochemistry212,436-445[Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair]

Dalev, P.G. and Simeonova, L.S.(1992),China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light. Biotechnology letters 14,6, 531-534[Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module]

Heidemann, E.(1997),Automatic CNC Glass Cutting Machine Glass Cutting Table.Leder 48,(5),99-104[2018 Trend Outdoor Products Fast Inflatable Air Sofa Bed Good Quality Sleeping Bag Inflatable Air Bag Lazy Bag Beach Sofa Laybag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Printing Adhesive Waterproof Custom Membrane Switch for Electric Cooker]

OEM Logo Soft Silicone Reusable BPA Free Drinking Straws, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory.Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken. USP/SP[Ysdcnc Manual Sheet Metal Plate Bending Roller Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Colorful Kinetic LED Vertical Tube DMX RGB Pixel Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.skrscale.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dark Green Plastic PVC Film for Christmas Tree Leaves]

Pet PP Rope and Broom Wire Drawing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD]

Pet PP Rope and Broom Wire Drawing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Logo Soft Silicone Reusable BPA Free Drinking Straws]

Pet PP Rope and Broom Wire Drawing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PTFE Seat Wafer Butterfly Valve Pn16, Part I.Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter]

China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China.Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01), 175-184[www.alitruck.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ASME VIII Pressure Vessel for Curing Carbon Fiber Parts]

Whitaker,J.R.(1963),Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. Analytical Chemistry35(12), 1950-1953[Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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