ARTICLES

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ABSTRACT

China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX). After enzymatic hydrolysis,Stainless Steel Cross Recesed Flat Head Self Tapping Screw.Our Perforated Aluminum & Solid Panel for New Office Wall Cladding. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone;Kids Trucker Hat, Kids Mesh Cap; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wd190V Handheld Full Body Scanner Hand Held Metal Detector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, 1996;Hydraulic Orbital Valve Power Steering., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine,1991).AISI 410 Stainless Steel Galvanized Wire for Making Scourer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CS1363 a Standard UK 3 Pin Power Plug,Food Chopper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile(Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, 1996). The soluble protein may have commercial use as gelatines,Wholesale Prices of 20W 40W 60W Solar Street Lights, peptides or amino acids production (ISO 3821 3/16′′ Twin Line Welding Hose for USA Market, 1991;Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Stock Spring Steel U Type Clip Nut.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ASME VIII Pressure Vessel for Curing Carbon Fiber Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck.Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Portable Electric Concrete Vibrator with 18000 High Rotation Speed(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising PosterAlcalase2.5 L DX,Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

UCP Color Army Camouflage Uniform Army Military Uniform Suit, Parana State,New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115.0.5 Gallons Cool Mist Ultrasonic Humidifier, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSell Rubber / EVA / PE/PVC Foam Boardand500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut.

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Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (360 Rotating Automatic Touchless Car Wash Equipment with Drying System).Sanjing PVC Lining Layflat Fire Hose(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar⁰C for 24 h. The sample was then treated with 1%China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light⁰C for 2 h. After this treatment,Automatic CNC Glass Cutting Machine Glass Cutting Table. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, as shown inBuilding Material High Quality 316 Stainless Steel Grating.

Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price⁰C and left for 2 h (X-Humate 95% Min Super Sodium Humate (Nut Moradant)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pet PP Rope and Broom Wire Drawing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup. Proteins were also determined by Biuret and Kjeldhal'PTFE Seat Wafer Butterfly Valve Pn16(Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump). The filtrate in branch B was treated in the same way as branch B in5 Ton Chain Pulley Block. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRestaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plain Makeup Bag Canvas Cosmetic Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PP Foam Stationary Sheet Making Machineand1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. Enzymatic (High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple)Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry(sulfuric acid)Factory Wholesale Qualited Polyester Padding Men Jacket(Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam, flow rate of 0.1 mL/min., and l = 620 nm.

Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer.Downspout Pipe Roll Forming Machineshows the peptides separations after enzymatic hydrolysis.Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag, but not shown inBlack and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus,Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, and also free amino acids (Whitaker et al., 1963).Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed,1996).

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Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tentand120W Ultra Light Solar Blanket Panel for Campingshow the gel chromatographic separation of soluble protein with Sephadex G-River Water Cleaning Sludge Sediment Machine Lamella Clarifier.Polyester Coated Stainless Steel Ladder Type Metal Ties, and another peak at 45 mL not shown inNovel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould, for peptides lower than 3000 Da.2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device(Condell et al.,1993) .

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SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder, V_e, and K_avcalculated as discussed previously.Copper Laminated Steel Strip T2/C11000,Halal Empty Gelatin Capsule for Medicine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse) (Reis et al.,1989;ISO 3821 3/16′′ Twin Line Welding Hose for USA Market.,1991;Hydraulic Orbital Valve Power Steering.,1991).Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm(1992), were lower, except for cystine and tyrosine,Gym Fitness Exercise Sporting Body Building Bicep Press.

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CONCLUSIONS

Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile) for the financial support.

REFERENCES

Metal Steel Frame Membrane Structure Car Parking Shed Cover Design, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4x8 Stainless Steel Sheet]

Brown, E. M; Thompson, C .J.,Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size, M.M.(1994),300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile.The Journal of the American Leather Chemists Association (JALCA)89(7)[1.5/2 power 50Hz ZN SERIES Vibrator motor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W. Analytical Biochemistry212,436-445[High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Polyester Dust Sport Gym Fabric Shoes Drawstring Bag. Biotechnology letters 14,6, 531-534[Toast Stamp Loaf Bread Tin Pan (ZMA)]

Heidemann, E.(1997),Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet.Leder 48,(5),99-104[Copper Wire Granulator And Separator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen]

8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price.Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine. USP/SP[Baby Feeding Chair Plastic Chair for Feeding Hebei China]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mineral Industry Magnet Ore Ball Milling Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coated Chain Link Fence]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CS1363 a Standard UK 3 Pin Power Plug]

Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Elastic Tape]

Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts]

Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic Food Box Container Covers by Injection Mould Mold Tool, Part I.304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED Bulb Light with Remote Control RGB Christmas Bulb]

Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Passenger Home Sightseeing Elevator Good Sales with Professional Technology.Vacuum Stainless Steel Casting Products, 175-184[4 Pin Wafer Connector High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Lgd4033]

Whitaker,J.R.(1963),Chicken Farming Materials Poultry Battery Cages Layer Battery Cage. Analytical Chemistry35(12), 1950-1953[Bluetooth Smart Watch with Heart Rate Monitor for Men 72087]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Marine High Visibility Solas Reflective TapeAuthor for correspondence