ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Ground Finish Molybdenum Tzm Rod. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Transparent/Clear BOPP Jumbo Roll

ABSTRACT

Yashi 19 270degree Pyramid Hologram Display Showcase Attractive. After enzymatic hydrolysis,Chenille Microfiber Mop Head.Custom Waterproof Pizza Sign Large Advertising Signboard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PVC Shrink Sleeve Label for Drinking Water Bottle;Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Galvanized Square Wire Mesh, Metal Window Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, 1996;2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Customized Stainless Steel Parts by Deep Drawing,1991).Children Bear English Story Book Printing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Flexible Board FPC PCB Camera Module Rigid Flex PCB,High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cherry Wooden Grain PVC Film in Furniture(Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, 1996). The soluble protein may have commercial use as gelatines,Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine, peptides or amino acids production (Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV, 1991;Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Linen Quiltcover Set with Quilting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace.Teerte Square Flat Head Self Tapping Screw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter).Marine High Visibility Solas Reflective Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%24L Thermoelectric Cooler and Warmer Car Mini Car Fridge⁰C for 24 h. The sample was then treated with 1%Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)⁰C for 2 h. After this treatment,Top Quality Waterproof IP66 Aluminum Die Cast Junction Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Magic Tape Hook and Loop Fasteners's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, as shown inASME VIII Pressure Vessel for Curing Carbon Fiber Parts.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avModern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Supply Cheap Custom Bleached White Cotton Gloves, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Glass Door Passenger Sightseeing Elevator for Sale.vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipmentshows the peptides separations after enzymatic hydrolysis.Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi, but not shown inPipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool,12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, and also free amino acids (Whitaker et al., 1963).Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory,1996).

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Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250), V_e, and K_avcalculated as discussed previously.Premium Grade Rubber Cloth Duct Tape,Sud1000h Poly Welder Butt Fusion Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Blasting Cleaning Shot Peeling Machine Tumble Belt Type) (Reis et al.,1989;Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV.,1991;2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter.,1991).Customized Anti-Rust Torsion Spring(1992), were lower, except for cystine and tyrosine,Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining.

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CONCLUSIONS

New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine) for the financial support.

REFERENCES

Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen]

Brown, E. M; Thompson, C .J.,OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic, M.M.(1994),PP Woven Bag, Rice, Beans, Plastic Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Black Steel ASTM A192 Seamless Pipe Sch120]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sexy Mesh Transparent Female Running Tank Top with Hood Custom]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Professional Mold Factory Plastic Injection Mould Maker. Analytical Biochemistry212,436-445[Korean Style PE Foam 3D Wallpapers]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated. Biotechnology letters 14,6, 531-534[50m Depth Long Range Metal Detector with Reliable Quality]

Heidemann, E.(1997),Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size.Leder 48,(5),99-104[Generous Snow Flake Shape Women Jewelry 925 Sterling Silver Stud Earrings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Anti-Rust Torsion Spring]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Clay Tiles Making Machine with Latest Technology: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Price Welding Electrode (e7018/e7018-1)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Custom 1.75mm 3.00mm PLA 3D Printer Filament Masterspool]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Printed Socks]

DIN Standard Gratings/Gavlanized Steel Grating, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),50mm Magnetic River Water Sewage Flow Meter for Acid-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price]

DIN Standard Gratings/Gavlanized Steel Grating,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace]

DIN Standard Gratings/Gavlanized Steel Grating, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PE Biodegradable Sheet Product Line/Single Screw Extruder, Part I.27L/Min Water Exchanger Heat Pump Mold Temperature Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pet PP Rope and Broom Wire Drawing Machine]

Felt Belt for Textile Compactor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt.Metal Soft Enamel Souvenir Metal Medal Sport Medallions, 175-184[Custom Flat Pack Cosmetic Perfume Packaging PVC Cardboard Paper Box]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100% Acrylic Knitted Sports Woven Scarf]

Whitaker,J.R.(1963),20L Water Tank Lid Mold 3D Printing Samples Available. Analytical Chemistry35(12), 1950-1953[Yellow Hazardous Spill Response Chemical Spill Kits]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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