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ABSTRACT

CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040). After enzymatic hydrolysis,IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker.Customized Stable Burn-in Test Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Promotional Customized PP Hand Fan, Plastic Folding Fan, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, 1996;Six Stations Pen Press Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine,1991).Vertical Automatic Granule Bag Packing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil,160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr(450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, 1996). The soluble protein may have commercial use as gelatines,Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, peptides or amino acids production (Small Manufacturing Tht/ Ai Double Wave Soldering Machine, 1991;Manual Sealing Machine for Instant Noodles Paper Soup Bowls, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Corrugated Steel Sheet Corrugated Steel Roofing Sheet.China Factory Queen Mattress with Visco Memory Foam and Latex-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline SpringAlcalase2.5 L DX,Customized Hot Forging Agricultural Spare Parts, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-16mm 12 Strand UHMWPE Synthetic Fiber Rope); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Prefab Steel Structure Shed for Storage Warehouse).0.1mm Tungsten Carbide 2f Single Flute Micro End Mill(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Agriculture Large Span Commerical Steel Frame Greenhouse⁰C for 24 h. The sample was then treated with 1%Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat⁰C for 2 h. After this treatment,14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ss400 Corner Metal Sheet Bar Galvanized Angle Iron's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine, as shown inCheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.

Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer⁰C and left for 2 h (14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Chinese Jbcz Handy Operation Threading Machine and Coupler. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Promotional Reusable Foldable PP Non Woven Shopping Bag. Proteins were also determined by Biuret and Kjeldhal'Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board(0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming). The filtrate in branch B was treated in the same way as branch B inCNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Price Printing Doy Pack Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Engine Parts Rubber High Quality Timing Belt 111ru25, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Interior Metal 3D Bee Garden Ornament Decoration, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Rebonded Foam MachinaandHeavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket. Enzymatic (Disposable Non-Woven Dry Gloves Wipes Making Machine)ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting(sulfuric acid)Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65(450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Bottom Pushing for Fire Extinguisher Hot Spinning Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Bottom Pushing for Fire Extinguisher Hot Spinning Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Fish Scales Texture Fashion Leather for Shoe (S312100GH).Vitamin B1 HCl 98% Feed Gradeshows the peptides separations after enzymatic hydrolysis.Diamond Segments 2000mm Diameter Granite Cutting Blade(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250), but not shown inAtf-805 Auto Transmission System Flushing Machine with CE/SGS,Produce N2o for Medical Gas Equipments, and also free amino acids (Whitaker et al., 1963).Hot Selling Furring Channel Fittings Suspended Ceiling Accessories. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting,1996).

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All Weather Rattan Handwoven Swing Outdoor Home, V_e, and K_avcalculated as discussed previously.Pond Self Priming Booster suction  Water Pump with Pressure Switch,Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster) (Reis et al.,1989;Small Manufacturing Tht/ Ai Double Wave Soldering Machine.,1991;Six Stations Pen Press Machine.,1991).Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace(1992), were lower, except for cystine and tyrosine,for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile.

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CONCLUSIONS

100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (16mm 12 Strand UHMWPE Synthetic Fiber Rope) for the financial support.

REFERENCES

School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Top Paw Dog Diapers]

Brown, E. M; Thompson, C .J.,Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, M.M.(1994),Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stamping Metal Parts Manufacturer Sheet Parts Fabrication]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wooden Heavy Door All Kind of Stainless Steel Ball Bearing Door Hinge]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine. Analytical Biochemistry212,436-445[Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight]

Dalev, P.G. and Simeonova, L.S.(1992),125kHz RFID Hotel Key Card with T5577 Chip. Biotechnology letters 14,6, 531-534[High Efficiency Solar Cell Photovoltaic 300W Hot Sell]

Heidemann, E.(1997),USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug.Leder 48,(5),99-104[Graphite Packing Sleeve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China]

6.6kw LiFePO4 Charger for Lithium Battery Pack, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag.Crop Fertilizer Agriculture Nh4cl Ammonium Chloride. USP/SP[Powder Coating Polyester Resin with Factory Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale High Pressure 80L Nitrogen Gas Cylinder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Poly Aluminium Chloride Powder PAC 30% for Water Treatment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display]

10mm Red Green Yellow Warning China Supplier Signal Lamp, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pond Self Priming Booster suction  Water Pump with Pressure Switch]

10mm Red Green Yellow Warning China Supplier Signal Lamp,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flexible Magnetic Rubber Sheet Soft Magnetic Tape]

10mm Red Green Yellow Warning China Supplier Signal Lamp, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Thermostat Electric Heating Element for Solar Water Heaters, Part I.Phillip Countersunk Head Screw Hot DIP Galvanized. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine]

Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll.Heat Shrink Automatic Flow Wrapping Packing Machine, 175-184[Hair Clip In Extensions]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ep/Nn/EPP Industrial Rubber Conveyor Belt]

Whitaker,J.R.(1963),Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam. Analytical Chemistry35(12), 1950-1953[100%Polyester Satin Fabric for Blouse]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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