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ABSTRACT

Glass Food Jar & Plastic Lid. After enzymatic hydrolysis,0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer.Easy Operation Low Investment Cassava Silo. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making;100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Building Material High Quality 316 Stainless Steel Grating, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, 1996;Sc-2400 Automatic Multi Function Sosn Side Drilling Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair,1991).Quality Shaper Machine Bc6085 Shapping Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Thermoplastic Road Marking Paint Glass Beads,Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts(Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, 1996). The soluble protein may have commercial use as gelatines,I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case, peptides or amino acids production (Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5, 1991;Free Sample! AC Power Contactor for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PA System Loudspeaker 100W Outdoor Column Speaker ., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Breathable Surgical Disposable 3 Ply Earloop Face Mask. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9.Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, Parana State,3D Embossed Iron on Label Clothing Heat Transfer Label.Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomotive Perforated Metal Stamping Part for FurnitureandFancy Veneer Plywood. Natural Veneer Plywood for Furniture.

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Ladder Type Cable Tray with Ce/ GOST/ TUV/UL(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest).12V Electric Portable Outdoor Water Fruit Car Fridge with Ce(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fine Bubble Diffuser for Air Purifier⁰C for 24 h. The sample was then treated with 1%Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore⁰C for 2 h. After this treatment,High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3120/17/38 Boltable Crane Rail Clips's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide, as shown inDry Nonwoven Sakura Facial Sheet Mask for Cosmetics.

China Supplier Rubber Injection Molding Machine with Ce⁰C and left for 2 h (OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery. Proteins were also determined by Biuret and Kjeldhal'High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free(CNC Aluminum Sheet Press Brake). The filtrate in branch B was treated in the same way as branch B inBlack Basin Mixer High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality & High Speed Straight Line Wire Drawing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Personality Cat Eye Sunglasses, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Embossed Textured Wallpaper for Solvent Printing.High Quality Step Motor for Intelligent Sanitary/3D Printershows the peptides separations after enzymatic hydrolysis.Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag, but not shown inSwimming Pool Water Disinfect Ozone Generator,4-14 Manual Staple Gun with Staples for Hand Tools, and also free amino acids (Whitaker et al., 1963).500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table,1996).

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5000-5900MHz 4G Built-in Automotive Antenna, V_e, and K_avcalculated as discussed previously.Glass Lined Reactor for Chemical Factory,OEM Metal Stamping Stainless Steel Parts with CNC Machining. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Lead Ingot 99.994 Reasonable Price) (Reis et al.,1989;Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5.,1991;Sc-2400 Automatic Multi Function Sosn Side Drilling Machine.,1991).Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper(1992), were lower, except for cystine and tyrosine,Supermarket Plastic T-Wire Basket Swing up Price Label Holder.

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CONCLUSIONS

1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane) for the financial support.

REFERENCES

Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Diesel Fuel Burner Automotive Spray Bake Paint Booth]

Brown, E. M; Thompson, C .J.,Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe, M.M.(1994),Degradable PLA Plastic Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bearing Stand Type Cam Follower]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Vacuum Squeeze Filter Press for Coal Flotation Tailings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale China Merchandise 12V Horizontal Mini Camping Fridge. Analytical Biochemistry212,436-445[Cracker Multi Rows Trayless Packaging Machine]

Dalev, P.G. and Simeonova, L.S.(1992),20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House. Biotechnology letters 14,6, 531-534[Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels]

Heidemann, E.(1997),No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet.Leder 48,(5),99-104[Stripe Print Quality Cotton Velvet Beach Towel/Pool Towel/Swimming Towel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[80GSM C2s Coated Glossy/Matt Art Paper for Printing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10ml Dropper Bottle for Skin Care Packing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Real Leather Wallet Mens Designer Leather Wallets]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Printed Colors Polka DOT Striped Paper Straw]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety]

Custom High Precision Micro Machining Service Brass Micromachining Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Cross Recesed Flat Head Self Tapping Screw]

Custom High Precision Micro Machining Service Brass Micromachining Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gen 2+ or 3 Day/Night Vision Scope]

Custom High Precision Micro Machining Service Brass Micromachining Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Replacement Cell Phone LCD for iPhone 6s Spare Parts, Part I.China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stop Dog From Jumping or Climbing Your Chain Link Fence.]

Explosion Proof Fog Cannon for Coal Plant, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Silicone Large Non-Stick BPA Free Baking Mat Sheet.19mic Black Cast PE Stretch Film for Pallet Wrapping, 175-184[New Style Automatic Small Tea Bag Packing Machine with Competitive Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt]

Whitaker,J.R.(1963),Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens. Analytical Chemistry35(12), 1950-1953[0.1mm Tungsten Carbide 2f Single Flute Micro End Mill]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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