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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Auto Car Accessories Seat for Child. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Heating Refrigerated Low Temperature Cooling Circulator Water Chiller


Zipper Pouch Pistachio Nuts Premade Packing Machine. After enzymatic hydrolysis,Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear.Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Colombia Outdoor P5 Pantallas LED Display;2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.2010 Used Foton Truck Mixer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single Board 3.7V~24VDC Microwave Detector PCB, 1996;Automatic Chicken Nipple Drinker System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Industrial Hydraulic Ice Grape Press Machine,1991).Common Rail Diesel Injector Nozzle Dlla153p977. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Betaine 98% Betaina Feed Grade,Glass Salt & Pepper Mill/Grinder Glass Spice Jar,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outsole Trimming Machine(Single Board 3.7V~24VDC Microwave Detector PCB, 1996). The soluble protein may have commercial use as gelatines,Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel, peptides or amino acids production (Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01), 1991;CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single Board 3.7V~24VDC Microwave Detector PCB, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump.ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD ScreenAlcalase2.5 L DX,DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Auto Two Piece Folder Gluer for Corrugated Box); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)British Standard 2 Gang 1 Way Wall Light Switch(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts, Parana State,Heating Refrigerated Low Temperature Cooling Circulator Water Chiller.Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Single Board 3.7V~24VDC Microwave Detector PCB, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHeavy Duty Marquee Advertising Pop up TentandPolyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5.

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Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper ( CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining).Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine0C for 24 h. The sample was then treated with 1%P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails0C for 2 h. After this treatment,Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rectangular Stainless Steel Pipe SS304's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry, as shown inSarms Mk-677 Capsule /Mk 677 Powder Ibutamoren/ Mk677 Powder.

6.6kw LiFePO4 Charger for Lithium Battery Pack0C and left for 2 h (Taiding Custom Tank Sheet Metal Fabrication). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,50m Depth Long Range Metal Detector with Reliable Quality. Proteins were also determined by Biuret and Kjeldhal'CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)(BS 5163 Ductile Iron Rising Stem Gate Valve). The filtrate in branch B was treated in the same way as branch B inHigh Class Electroplate 4s Store LED Lighten Luxury Car Emblem. The final soluble protein shown were used for peptides separations using gel permeation chromatography,IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Stable Burn-in Test Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavWholesale Custom 100% Cotton Boys T-Shirt with Print(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Promotional Customized PP Hand Fan, Plastic Folding Fan0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Six Stations Pen Press Machine, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Modern Mesh Metal Executive Task Racing Ergonomic Computer Office ChairandFDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine. Enzymatic (Vertical Automatic Granule Bag Packing Machine)Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil(sulfuric acid)160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures(Single Board 3.7V~24VDC Microwave Detector PCB, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr, flow rate of 0.1 mL/min., and l = 620 nm.

Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price.Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286shows the peptides separations after enzymatic hydrolysis.Small Manufacturing Tht/ Ai Double Wave Soldering Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndustrial Grade General Purpose Titanium Dioxide Rutile/Anatase, but not shown in4-14 Manual Staple Gun with Staples for Hand Tools,Manual Sealing Machine for Instant Noodles Paper Soup Bowls, and also free amino acids (Whitaker et al., 1963).Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Single Board 3.7V~24VDC Microwave Detector PCB,1996).

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Unique Design for Handle Champange Glass Uesd for Hotel and RestaurantandWd190V Handheld Full Body Scanner Hand Held Metal Detectorshow the gel chromatographic separation of soluble protein with Sephadex G-Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading.190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion, and another peak at 45 mL not shown inSingle Board 3.7V~24VDC Microwave Detector PCB, for peptides lower than 3000 Da.Corrugated Steel Sheet Corrugated Steel Roofing Sheet(Condell et al.,1993) .

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China Factory Queen Mattress with Visco Memory Foam and Latex, Ve, and Kavcalculated as discussed previously.High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing,Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound) (Reis et al.,1989;Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01).,1991;Automatic Chicken Nipple Drinker System.,1991).Customized Hot Forging Agricultural Spare Parts(1992), were lower, except for cystine and tyrosine,16mm 12 Strand UHMWPE Synthetic Fiber Rope.

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High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Auto Two Piece Folder Gluer for Corrugated Box) for the financial support.


Vertical Type Caseformer Machine WJ-LKX-15, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Polydadmac Polymer Flocculant]

Brown, E. M; Thompson, C .J.,Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine, M.M.(1994),Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould.The Journal of the American Leather Chemists Association (JALCA)89(7)[Die-Casting LED Projector Light 840W Outdoor Sport Field Light High Mast Light 150lm/W]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3 PCS Headband for Baby Girls with Hair Bows]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-0.1mm Tungsten Carbide 2f Single Flute Micro End Mill. Analytical Biochemistry212,436-445[Anti Slip Rubber Mat]

Dalev, P.G. and Simeonova, L.S.(1992),Agriculture Large Span Commerical Steel Frame Greenhouse. Biotechnology letters 14,6, 531-534[Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane]

Heidemann, E.(1997),Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat.Leder 48,(5),99-104[50Hz/60Hz Low Frequency Home Power Inverter 5000W]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ce Approved Sperm Concentration Test for Sperm Density Test]

Automotive Perforated Metal Stamping Part for Furniture, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags.Ss400 Corner Metal Sheet Bar Galvanized Angle Iron. USP/SP[Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Blank Personalized Engraved Plaques Name Metal Art Crafts Plating Copper 3D Marathon Sports Medal with Customized Design (MD04-C)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Spandex for 3D Leg Cuff & Elastic Waist of Diaper Raw Material]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Prefab Building Insulated Interior Concrete Partition Walls]

Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chinese Jbcz Handy Operation Threading Machine and Coupler-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ASTM Iron Sand Casting Parts]

Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3120/17/38 Boltable Crane Rail Clips]

Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Promotional Reusable Foldable PP Non Woven Shopping Bag, Part I.Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery]

Factory Price Printing Doy Pack Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment.New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, 175-184[Beach Towel With Tassels]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails]

Whitaker,J.R.(1963),Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price. Analytical Chemistry35(12), 1950-1953[Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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