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m.gilardinoflooring.comEdivaldo E.Garcia^I; Cláudia C.M.Gym Fitness Exercise Sporting Body Building Bicep Press^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{High Performance Multifunctional Crawler Mini Skid Steer Loader}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Metal Steel Frame Membrane Structure Car Parking Shed Cover Design

ABSTRACT

Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size. After enzymatic hydrolysis,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile.Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Polyester Dust Sport Gym Fabric Shoes Drawstring Bag;Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, 1996;Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank,1991).Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Food Box Container Covers by Injection Mould Mold Tool,304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans(Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, 1996). The soluble protein may have commercial use as gelatines,Passenger Home Sightseeing Elevator Good Sales with Professional Technology, peptides or amino acids production (Vacuum Stainless Steel Casting Products, 1991;Chicken Farming Materials Poultry Battery Cages Layer Battery Cage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.I-Tech 12000HD 2u Space Digital Professional Power Amplifier.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Silicone Reusable Food Storage Bag China Manufacturer.Polyurethane Gasket Foam Seal Dispensing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Imee Print Customized Printing Agenda Printing Nb21(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Xjm Series Rubber Extruding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box).Stop Dog From Jumping or Climbing Your Chain Link Fence.(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SGS Direct Thermal Label for Supermarket⁰C for 24 h. The sample was then treated with 1%Water Filter Polyester Non-Woven Fabric⁰C for 2 h. After this treatment,Industry Pet Recycling Systems, Industry Pet Recycling Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sublimation Polyester Sports Tshirt for Adult's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Heavy Duty Marquee Advertising Pop up Tent, as shown inHot Sale Laser Projector RGB Laser Light for Stage.

Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer⁰C and left for 2 h (Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fire Extinguisher UV Curing Machine Cylinder Drying Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. Proteins were also determined by Biuret and Kjeldhal'Energy-Saving Biogas Double Stove for Sale(Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength). The filtrate in branch B was treated in the same way as branch B inMetal Mesh Grate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Valve Bag Dry Mortar Bagging Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality 500m 100lb Super Strong Braided Fishing Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Lost Wax Casting Precision Casting Spring Snap Hooks for Chain RiggingandSheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending. Enzymatic (Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch)Custom Stainless Steel Jewelry Production by Metal Injection Molding Process(sulfuric acid)Factory Directly Selling Engine Standard Spark Plug(Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Logo Silicone Wristband, Rubber Wrist Bands, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Logo Silicone Wristband, Rubber Wrist Bands, flow rate of 0.1 mL/min., and l = 620 nm.

Custom All Kinds of Acrylic Shoe Display Racks.ID25mm Od86mm Standard Through Hole Slip Ringshows the peptides separations after enzymatic hydrolysis.Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, but not shown inDigital Lock Electronic Door Lock Password Keypad Number for Cabinet Door,Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale, and also free amino acids (Whitaker et al., 1963).Medical Supply U40 Disposable Insulin Syringe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine,1996).

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Stainless Steel Precision Investment Casting Valve Parts for Transmission GearboxandGood Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truckshow the gel chromatographic separation of soluble protein with Sephadex G-Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine.Water Chiller Air Cooled Chiller Industrial Chiller for Sale, and another peak at 45 mL not shown inH2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211), for peptides lower than 3000 Da.Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair(Condell et al.,1993) .

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High Precision Tungsten Carbide Machine Customized Cutting Tool, V_e, and K_avcalculated as discussed previously.I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box,Maleic Anhydride CAS 108-31-6. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Expansion Anchor Bolt Steel Zinc Plated) (Reis et al.,1989;Vacuum Stainless Steel Casting Products.,1991;Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver.,1991).2000mj Q Switched for Pigment Removal with Ce Certification(1992), were lower, except for cystine and tyrosine,Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured.

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CONCLUSIONS

Cow Dung Chicken Manure Organic Fertilizer Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar) for the financial support.

REFERENCES

marble Makeup Brush Set for Travel in 20PCS, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Graphite Crucible Liner]

Brown, E. M; Thompson, C .J.,Swimming Pool Water Disinfect Ozone Generator, M.M.(1994),20kv 50kVA High Voltage Dry Type Power Supply Transformer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industry 0.002mm Accuracy Checking Fixture]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Impact Sound Isolation Wall Clip]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp. Analytical Biochemistry212,436-445[AISI 410 Stainless Steel Galvanized Wire for Making Scourer]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter. Biotechnology letters 14,6, 531-534[Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024]

Heidemann, E.(1997),4 Styles Blue Glass Crystal Butt Beads Spiral Plug.Leder 48,(5),99-104[Industrial Food Canning Machine Food for Meatball]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter]

Gym Fitness Exercise Sporting Body Building Bicep Press, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve.Industrial Activated Carbon Water Filter Industry Water Treatment. USP/SP[B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fully Automatic C Z Purlin Cold Roll Forming Machinery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2016 New Generation Sausage Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Residential Artificial Grass for Garden Landscaping Artificial Grass]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap Alloy Snap Hook Clasps]

Magnetic Mixer Mixing Tank with Bottom Agitator (MTK), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chicken Slaughtering Machine for Sale]

Magnetic Mixer Mixing Tank with Bottom Agitator (MTK),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm]

Magnetic Mixer Mixing Tank with Bottom Agitator (MTK), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03), Part I.China Supplier Building Construction Steel Frame (ZY431). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug]

iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Lab Equipment portable Fume Hood.Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, 175-184[Electric Car Charging Station EV Charging Wall Mount 11kw]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Thermoplastic Road Marking Paint Glass Beads]

Whitaker,J.R.(1963),10ton Telescopic Hydraulic Truck Crane. Analytical Chemistry35(12), 1950-1953[Galvanized Hexagonal Wire Mesh Gabion / Gabion Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ultrasonic Automatic Face Mask Blank Making MachineAuthor for correspondence