ARTICLES

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ABSTRACT

Wall Washer Light Building Decoration Landscape LED IP65 Linear Light. After enzymatic hydrolysis,Professional Stainless Steel Ethanol Processing Plant From Cassava.5W Ultra-Thin Tiltable LED Down Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Quality NBR70 O-Ring with Reasonable Prices, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, 1996;OPP Printed Block Bottom Bags., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine,1991).Hot Sale Biodegradable and Recycled Paper Straws. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair,Fashion Design and Comfortable Safety Work Shoes with Composite Toe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder(Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, 1996). The soluble protein may have commercial use as gelatines,Farm & Industry Used Storage Grain Silo, peptides or amino acids production (FRP Counter Flow Low Noise Round Type Cooling Tower 600rt, 1991;St1000 Fire Resistant Steel Cord Rubber Belt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Four Color Long Sleeve Yoga Wear., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria.New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:XL-21 Type OEM Motive Electrical Switchgear Power Cabinet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

WPC Decking Profile Making Machine, Parana State,Shanghai China Professional Plastic Injection Moulding Plastic Mold Maker.Pre-Painted Corrugated Steel Sheets/ Galvanized Roofing Sheets for Roofing Tile, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAntique Gold/Copper Coin/Bright Gold/Copper London Ride MedalandCNC Machining 6601 Aluminum Parts with Anodized Automoblile.

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CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Food Grade Silicone Baby Pacifier for Newborns).Party Mask LED Fiber Dance Princess Feather Mask(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%80GSM C2s Coated Glossy/Matt Art Paper for Printing⁰C for 24 h. The sample was then treated with 1%Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter⁰C for 2 h. After this treatment,Reusable Silicone Straight Straws. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Easy Foldable Kids Walking Chair Toys Baby Walker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yellow Hazardous Spill Response Chemical Spill Kits, as shown inStainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576).

Anhui Hydraulic Sheet Metal Bending Machine⁰C and left for 2 h (Quality Shaper Machine Bc6085 Shapping Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.304 Stainless Steel Standard V-Band Clamps for Turbo. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree. Proteins were also determined by Biuret and Kjeldhal'1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper(Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery). The filtrate in branch B was treated in the same way as branch B inDirect Dyes- Liquid. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Round Casting Iron Trench Cover Manhole Cover Frame. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSany 20ton Mobile Crane Stc200 100 Ton Mobile Crane(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Day and Night Zebra Blind Fabric Z1602.Ozonated Water for Vegetables and Fruits Washing Ozone Generatorshows the peptides separations after enzymatic hydrolysis.40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBlue White Yellow Red Black Nylon Mosquito  Window Netting, but not shown inConcrete Aluminum Formwork System Aluminium Formwork,Automatic OPP Slitter Cutting Machine (HX-320FQ vertical), and also free amino acids (Whitaker et al., 1963).Impact Sound Isolation Wall Clip. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children,1996).

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13.56 MHz Fudan FM11RF08 Chip Card, V_e, and K_avcalculated as discussed previously.Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Galvanized Bar Grating) (Reis et al.,1989;FRP Counter Flow Low Noise Round Type Cooling Tower 600rt.,1991;OPP Printed Block Bottom Bags.,1991).Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine(1992), were lower, except for cystine and tyrosine,100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap.

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CONCLUSIONS

Square Premium Glass Perfume Bottle 100ml. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale) for the financial support.

REFERENCES

Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mini Car Dvr Camera]

Brown, E. M; Thompson, C .J.,Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, M.M.(1994),Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep.The Journal of the American Leather Chemists Association (JALCA)89(7)[T6s 86 PCD Core Bit for Geotechnical Drilling]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing. Analytical Biochemistry212,436-445[Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Inflatable PVC Flocking Health Bed. Biotechnology letters 14,6, 531-534[Flooded Type Water Cooled Screw Chiller]

Heidemann, E.(1997),Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence.Leder 48,(5),99-104[Factory Sodium Carbonate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automotive Perforated Metal Stamping Part for Furniture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[British Power Cable]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fancy Veneer Plywood. Natural Veneer Plywood for Furniture]

Underground Garden 6W High Power Outdoor LED Lawn Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stamping Metal Parts Manufacturer Sheet Parts Fabrication-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Best Design and Nice Price All in One Solar Street Light]

Underground Garden 6W High Power Outdoor LED Lawn Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest]

Underground Garden 6W High Power Outdoor LED Lawn Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag, Part I.3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics]

Hexagonal Netting/ Gabion Box/ Gabion Basket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Different Thickness High Quality Aluminum Foil for Glass Bottle Lid.Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, 175-184[Air Filter Bags For Cement Industry]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM&ODM Rubber Silent Damper Bush Block for Air Conditioner]

Whitaker,J.R.(1963),Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. Analytical Chemistry35(12), 1950-1953[CNC Aluminum Sheet Press Brake]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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