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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine. After enzymatic hydrolysis,Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending.Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Stainless Steel Jewelry Production by Metal Injection Molding Process;Factory Directly Selling Engine Standard Spark Plug; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Custom Logo Silicone Wristband, Rubber Wrist Bands, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom All Kinds of Acrylic Shoe Display Racks, 1996;Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale,1991).Medical Supply U40 Disposable Insulin Syringe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine,Water Chiller Air Cooled Chiller Industrial Chiller for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair(Custom All Kinds of Acrylic Shoe Display Racks, 1996). The soluble protein may have commercial use as gelatines,High Precision Tungsten Carbide Machine Customized Cutting Tool, peptides or amino acids production (I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, 1991;Maleic Anhydride CAS 108-31-6, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2000mj Q Switched for Pigment Removal with Ce Certification., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cow Dung Chicken Manure Organic Fertilizer Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom All Kinds of Acrylic Shoe Display Racks, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10marble Makeup Brush Set for Travel in 20PCS.Swimming Pool Water Disinfect Ozone Generator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:20kv 50kVA High Voltage Dry Type Power Supply Transformer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Industrial Activated Carbon Water Filter Industry Water Treatment, Parana State,PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint.2016 New Generation Sausage Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Custom All Kinds of Acrylic Shoe Display Racks, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theThe Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game MachineandSide Pack Manual Gas Engine Power Brush Cutter.

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Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines).Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)0C for 24 h. The sample was then treated with 1%China Supplier Building Construction Steel Frame (ZY431)0C for 2 h. After this treatment,iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Lab Equipment portable Fume Hood's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt, as shown inDay and Night Zebra Blind Fabric Z1602.

10ton Telescopic Hydraulic Truck Crane0C and left for 2 h (Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display. Proteins were also determined by Biuret and Kjeldhal'Cracker Multi Rows Trayless Packaging Machine(Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)). The filtrate in branch B was treated in the same way as branch B inwww.let-dcpump.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2 Quick Change Flexible Roloc Disc. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavUTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PRO-Installer Fiber Optic Termination Kit Lk-6016, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Effluent Treatment Ferrous Sulphate Heptahydrate 98%andSteel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace. Enzymatic (Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901))Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs(sulfuric acid)Powder Coated H Column Beam Steel Structure Building for Sale(Custom All Kinds of Acrylic Shoe Display Racks, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, flow rate of 0.1 mL/min., and l = 620 nm.

Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine.Vacuum Stainless Steel Casting Productsshows the peptides separations after enzymatic hydrolysis.500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLow Volume Large Variety Non Standard Customized High Precision CNC Machining Parts, but not shown inGuided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam,Nylon Cable Glands with CE, and also free amino acids (Whitaker et al., 1963).X Shaped Extruded Aluminum Window Screen Frames. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom All Kinds of Acrylic Shoe Display Racks,1996).

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Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (4 Styles Blue Glass Crystal Butt Beads Spiral Plug) for the financial support.


H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Medal Hanger Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet, M.M.(1994),High Quality Crusher for Used Plastic Recycling Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit. Analytical Biochemistry212,436-445[ LED Bayomet Vehicle Lamps]

Dalev, P.G. and Simeonova, L.S.(1992),SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving. Biotechnology letters 14,6, 531-534[Phillip Countersunk Head Screw Hot DIP Galvanized]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic CNC Glass Cutting Machine Glass Cutting Table]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flat Surface Mounted 300mm 24W LED Panel Light for Interior: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[99.995%-99.999% Carbon Dioxide Gas Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[18X16mesh Fiberglass Window Screen Insect Screen]

Latex Paint Emulsion Thickener Agents with High Leveling Property, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap]

Latex Paint Emulsion Thickener Agents with High Leveling Property,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[FRP Counter Flow Low Noise Round Type Cooling Tower 600rt]

Latex Paint Emulsion Thickener Agents with High Leveling Property, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Orfs Male/Orfs Female Hose Fitting, Part I.Ss400 Galvanized Steel C Channel Steel Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pipe Conveyor Belt]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hydraulic Orbital Valve Power Steering]

Whitaker,J.R.(1963),High Quality Step Motor for Intelligent Sanitary/3D Printer. Analytical Chemistry35(12), 1950-1953[Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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