ARTICLES

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ABSTRACT

Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon. After enzymatic hydrolysis,High Quality Ceramic Chrome Hybrid Bearing Oil.Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder;Digger Arm Small Wheel Loader (HQ908) with CE; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, 1996;Water Based Emulsion Paint Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cnm Single Strand Anchor Coupler,1991).Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,18X16mesh Fiberglass Window Screen Insect Screen,Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sterile Dsiposable Syringe for Dental 12ml(Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, 1996). The soluble protein may have commercial use as gelatines,Foldable Bed Military Cot for Camping, peptides or amino acids production (P5 960*960mm LED Display Screen SMD Indoor /Outdoor, 1991;2019 New Advertising Inflatables Air Walking Backpack Ball, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Side Pack Manual Gas Engine Power Brush Cutter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Punching Bending Welding Steel Work Fabrication.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears.1 Ton Chain Pulley Block with Geared Trolley-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Powder Coating Polyester Resin with Factory Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

180W 1850mm*80mm Reci Laser Tube, Parana State,Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap.OEM Factory Balloon Padding Textile Machinery, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOriginal Cummins Diesel Engine Spare Parts Flywheel Ring GearandElectrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength.

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NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ID25mm Od86mm Standard Through Hole Slip Ring).Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine⁰C for 24 h. The sample was then treated with 1%Outdoor Concert Stage Equipment Aluminum Stage Portable Stage⁰C for 2 h. After this treatment,Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)22*30mm 12SMD 5050 LED Reading Light for Car's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital, as shown inCheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device.

Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness⁰C and left for 2 h (Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor Cheap Portable Stage Aluminum Truss Stage. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,One Arm Motorized Multifunction Hospital Equipment for China Supply. Proteins were also determined by Biuret and Kjeldhal'Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts(Comfortable Two Piece Women Fitness Clothing for Gym Wear). The filtrate in branch B was treated in the same way as branch B inSide Hole CNC Cutting Machine/CNC Router Drilling Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Printed Wristband Cotton Wristband RFID Woven Wristband⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dolphin Designed Handheld Massager (LC-889), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainless Steel Precision Investment Casting Valve Parts for Transmission GearboxandReal Leather Wallet Mens Designer Leather Wallets. Enzymatic (ASTM A240 321 Stainless Steel Sheet Price Per Kg)V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor(sulfuric acid)Promotional Plain Silver Empty Hinged Rectangular Tin Box(Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, flow rate of 0.1 mL/min., and l = 620 nm.

5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer.Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truckshows the peptides separations after enzymatic hydrolysis.Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inH2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211), but not shown inHeat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe,11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale, and also free amino acids (Whitaker et al., 1963).Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output,1996).

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Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, V_e, and K_avcalculated as discussed previously.Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security,Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (AISI 410 Stainless Steel Galvanized Wire for Making Scourer) (Reis et al.,1989;P5 960*960mm LED Display Screen SMD Indoor /Outdoor.,1991;Water Based Emulsion Paint Production Line.,1991).Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval(1992), were lower, except for cystine and tyrosine,High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump.

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CONCLUSIONS

10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (IEC 62196-2 Type 2 EV Charging 32A Male Plug) for the financial support.

REFERENCES

300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PP PE PS Pet PC ABS Plastic Sheet Extrusion Machine]

Brown, E. M; Thompson, C .J.,BS 5163 Ductile Iron Rising Stem Gate Valve, M.M.(1994),CNC Aluminum Sheet Press Brake.The Journal of the American Leather Chemists Association (JALCA)89(7)[B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fully Automatic C Z Purlin Cold Roll Forming Machinery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ID25mm Od86mm Standard Through Hole Slip Ring. Analytical Biochemistry212,436-445[Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection]

Dalev, P.G. and Simeonova, L.S.(1992),Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding. Biotechnology letters 14,6, 531-534[Cheap Alloy Snap Hook Clasps]

Heidemann, E.(1997),Bio Media.Leder 48,(5),99-104[Stainless Steel 304 Tubing Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chicken Slaughtering Machine for Sale]

Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine.Platform Trolley with 150kg Capacity, 4 Castor Wheel. USP/SP[Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Thermoplastic Road Marking Paint Glass Beads]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Climatic Cycling Stability Testing Chamber Temperature Humidity Cabinet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Galvanized Hexagonal Wire Mesh Gabion / Gabion Box]

LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),All Size Colored Tapping Hex Head Self Drill Screw Wood Screw-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ultrasonic Automatic Face Mask Blank Making Machine]

LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding]

LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pre-Engineered Cage Broiler Poultry Farm Building, Part I.Alumina Ball with High Quality. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel]

PVC Textile Fabric for Outdoor Sports Waterproof Backpack, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),White 4*8FT 18mm PVC Foam Board for Cabinets Use.Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, 175-184[China Ups Inverter Battery Charger]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Top Quality Galvanized Iron Wire as Binding Wire]

Whitaker,J.R.(1963),Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants. Analytical Chemistry35(12), 1950-1953[Alumina Balls for Hydogen Peroxide]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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