ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Stainless Steel Counter Top Working Table Chiller. After enzymatic hydrolysis,Cashew Grading Machine for Cashew Processing Line.4X4 Wd Car Tail Light for Ranger 2012-2016. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Power Press Perforated Metal Punch Machine, 1996;100%Polyester Satin Fabric for Blouse., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure,1991).Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High-End Compression Rash Guards, Long Sleeve Compression Shirt,High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office(Hot Sale Power Press Perforated Metal Punch Machine, 1996). The soluble protein may have commercial use as gelatines,Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters, peptides or amino acids production (9~12 Inch Mechanical Backing Plate, 1991;DIN929 Low-Cost Supply Hexagon Weld Nut, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Efficiency Solar Cell Photovoltaic 300W Hot Sell.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-60m3/H Lamella Clarifier for River Water Treatment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Power Press Perforated Metal Punch Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019).Super Fireproof Antibiosis Household Spc Vinyl Floor Plank-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Medical Supply U40 Disposable Insulin Syringe).Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%X-Humate 95% Min Super Sodium Humate (Nut Moradant)⁰C for 24 h. The sample was then treated with 1%Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board⁰C for 2 h. After this treatment,Women Summer Swimsuit Chest Zip Bathing Suit. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hemp Seed Oil Body Lotion Moisturizes The Skin's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh, as shown inManhole Galvanized Steel Trench Cover for Drain.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMedical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Must 4kw 2HP Pump Power Inverter Ags Bts Available, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Small Steel Silo for Poultry Feed, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight.Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Boardshows the peptides separations after enzymatic hydrolysis.Barbed Wire Right off The Border Wall to Protect Homes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, but not shown inAll Weather Rattan Handwoven Swing Outdoor Home,Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen, and also free amino acids (Whitaker et al., 1963).OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Power Press Perforated Metal Punch Machine,1996).

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CONCLUSIONS

Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W) for the financial support.

REFERENCES

Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Design Bag Accessory Rectangle Shape Metal Brand Zip Puller Tab]

Brown, E. M; Thompson, C .J.,Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml, M.M.(1994),Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Selling Military Large Event Marquee Tent Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automotive Perforated Metal Stamping Part for Furniture. Analytical Biochemistry212,436-445[Wholesale Custom Football Net Soccer Goal Net for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Auto Car Accessories Seat for Child. Biotechnology letters 14,6, 531-534[Modern Vertica Parking Garage System]

Heidemann, E.(1997),Heating Refrigerated Low Temperature Cooling Circulator Water Chiller.Leder 48,(5),99-104[Copper Hammered Antique Spa Ink]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration]

Milk Cream Disc Centrifugal Separator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Zipper Pouch Pistachio Nuts Premade Packing Machine.Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear. USP/SP[Color Cardstock Color Paper Manufacturer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manual Sealing Machine for Instant Noodles Paper Soup Bowls]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[316l Precision Steel Tube Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes]

Colombia Outdoor P5 Pantallas LED Display, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing]

Colombia Outdoor P5 Pantallas LED Display,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring]

Colombia Outdoor P5 Pantallas LED Display, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2010 Used Foton Truck Mixer, Part I.Single Board 3.7V~24VDC Microwave Detector PCB. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes]

Automatic Chicken Nipple Drinker System, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial Hydraulic Ice Grape Press Machine.Common Rail Diesel Injector Nozzle Dlla153p977, 175-184[Petrol Station Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer]

Whitaker,J.R.(1963),Betaine 98% Betaina Feed Grade. Analytical Chemistry35(12), 1950-1953[Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Magnetic Mixer Mixing Tank with Bottom Agitator (MTK)Author for correspondence