Services on Demand
Journal
Article
Indicators
- Sublimation Polyester Sports Tshirt for Adult
- Yashi 19 270degree Pyramid Hologram Display Showcase Attractive
Related links
- Heavy Duty Marquee Advertising Pop up Tent
Share
China Supplier Rubber Injection Molding Machine with Ce
Print versionISSN1516-8913
Braz. arch. biol. technol. vol.42 no.3 Curitiba 1999
Custom High Precision Micro Machining Service Brass Micromachining Parts
ARTICLES
Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer
Power Industry HDG Cable TrayEdivaldo E.GarciaI; Cláudia C.M.Fire Extinguisher UV Curing Machine Cylinder Drying MachineI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiCustom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close
IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Energy-Saving Biogas Double Stove for Sale
ABSTRACT
Valve Bag Dry Mortar Bagging Machine. After enzymatic hydrolysis,High Quality 500m 100lb Super Strong Braided Fishing Line.20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Key words;37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light;Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags; chromatography; protein, chromium
RESUMO
A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.
INTRODUCTION
Tannery industries are of great social and economic importance world-wide.PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, 1996;Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch,1991).Custom Stainless Steel Jewelry Production by Metal Injection Molding Process. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Directly Selling Engine Standard Spark Plug,Custom Logo Silicone Wristband, Rubber Wrist Bands,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).
Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom All Kinds of Acrylic Shoe Display Racks(Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, 1996). The soluble protein may have commercial use as gelatines,Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide, peptides or amino acids production (Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale, 1991;Medical Supply U40 Disposable Insulin Syringe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine., 1998).
This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Water Chiller Air Cooled Chiller Industrial Chiller for Sale.
MATERIALS AND METHODS
Apparatus
Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10High Precision Tungsten Carbide Machine Customized Cutting Tool.I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Maleic Anhydride CAS 108-31-6(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).
Reagents
2000mj Q Switched for Pigment Removal with Ce CertificationAlcalase2.5 L DX,Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Cow Dung Chicken Manure Organic Fertilizer Making Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)marble Makeup Brush Set for Travel in 20PCS(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).
Sample collection and preparation
Swimming Pool Water Disinfect Ozone Generator, Parana State,Energy-Saving Biogas Double Stove for Sale.20kv 50kVA High Voltage Dry Type Power Supply Transformer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomatic Three Side Sealing Facial Mask Bag Making MachineandYutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt.
Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply
450/750V Braided Shielded Flexible Control Cable PVC Insulation Copper Wire/Cable
Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple).High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4 Styles Blue Glass Crystal Butt Beads Spiral Plug0C for 24 h. The sample was then treated with 1%Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve0C for 2 h. After this treatment,Industrial Activated Carbon Water Filter Industry Water Treatment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2016 New Generation Sausage Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Magnetic Mixer Mixing Tank with Bottom Agitator (MTK), as shown inHigh Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding.
Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent0C and left for 2 h (5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,China Supplier Building Construction Steel Frame (ZY431). Proteins were also determined by Biuret and Kjeldhal'iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing(Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory). The filtrate in branch B was treated in the same way as branch B in1000x Biological Microscope Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Lab Equipment portable Fume Hood.
Chromatographic separation
Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.
The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav10ton Telescopic Hydraulic Truck Crane(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).
Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV(HPLC)
The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cracker Multi Rows Trayless Packaging Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.
RESULTS AND DISCUSSION
Professional Gym Equipment Hammer Strength PRO Style Adjustable Benchand2 Quick Change Flexible Roloc Disc. Enzymatic (UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack)High Quality Aluminium Windows Thermal Break China Supplier(sulfuric acid)Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines(Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01, flow rate of 0.1 mL/min., and l = 620 nm.
PRO-Installer Fiber Optic Termination Kit Lk-6016.Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tipshows the peptides separations after enzymatic hydrolysis.Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGlass Salt & Pepper Mill/Grinder Glass Spice Jar, but not shown inAll Size Colored Tapping Hex Head Self Drill Screw Wood Screw,Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), and also free amino acids (Whitaker et al., 1963).Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine,1996).
Low Frequency 3000W 24V 230V Inverter with Battery Charger
Ss400 Galvanized Steel C Channel Steel PriceandSchool Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)show the gel chromatographic separation of soluble protein with Sephadex G-Powder Coated H Column Beam Steel Structure Building for Sale.Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, and another peak at 45 mL not shown inH. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera, for peptides lower than 3000 Da.Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine(Condell et al.,1993) .
500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut, Ve, and Kavcalculated as discussed previously.Nylon Cable Glands with CE,X Shaped Extruded Aluminum Window Screen Frames. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Mobile Smartphone for Xiaomi 9 PRO) (Reis et al.,1989;Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale.,1991;Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending.,1991).Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair(1992), were lower, except for cystine and tyrosine,Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4.
Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver
IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker
CONCLUSIONS
4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.
ACKNOWLEDGMENT
The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cow Dung Chicken Manure Organic Fertilizer Making Machine) for the financial support.
REFERENCES
22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Waterproof Colored Fabric Mosquito Plastic Window Mosquito Netting]
Brown, E. M; Thompson, C .J.,Yashi 98 Inch Display Multi Touch Interactive Whiteboard LCD, M.M.(1994),Low Power Consumption Laser Foam Vertical Die Cutting Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud]
Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting]
Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug. Analytical Biochemistry212,436-445[PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint]
Dalev, P.G. and Simeonova, L.S.(1992),Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse. Biotechnology letters 14,6, 531-534[PVC Shrink Sleeve Label for Drinking Water Bottle]
Heidemann, E.(1997),Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity.Leder 48,(5),99-104[24v Pwm Brush Dc Motor điều khiển]
Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm]
Fire Extinguisher UV Curing Machine Cylinder Drying Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211).Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet. USP/SP[Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank]
O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)]
Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Crusher for Used Plastic Recycling Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters]
Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[E308lt1-1 Blue Arc Mig Welding Wire]
Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Biodegradable and Recycled Paper Straws]
High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Construction & Decoration ERW Square Steel Tube Manufacturer]
High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[M4X10 Round Head Core Pulling Aluminum Nail]
High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wear Resitant Rope End Cap Plastic Flange Cover, Part I.Comfortable Two Piece Women Fitness Clothing for Gym Wear. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price]
Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Flat Surface Mounted 300mm 24W LED Panel Light for Interior.Latex Paint Emulsion Thickener Agents with High Leveling Property, 175-184[Fast Construction Long Span Steel Frame Warehouse Building Design]
Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Metal Window Screen]
Whitaker,J.R.(1963),Fresh Juice with Suagr Filling Bottling Machine Small Scale Low Prices. Analytical Chemistry35(12), 1950-1953[High Quality Casting Cover Aluminum Die Casting Products]
Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.
PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator MachineAuthor for correspondence