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ABSTRACT

5W Ultra-Thin Tiltable LED Down Light. After enzymatic hydrolysis,Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8).1.5/2 power 50Hz ZN SERIES Vibrator motor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OPP Printed Block Bottom Bags, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine, 1996;Hot Sale Biodegradable and Recycled Paper Straws., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair,1991).Fashion Design and Comfortable Safety Work Shoes with Composite Toe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder,Farm & Industry Used Storage Grain Silo,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,FRP Counter Flow Low Noise Round Type Cooling Tower 600rt(CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine, 1996). The soluble protein may have commercial use as gelatines,St1000 Fire Resistant Steel Cord Rubber Belt, peptides or amino acids production (Four Color Long Sleeve Yoga Wear, 1991;Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰XL-21 Type OEM Motive Electrical Switchgear Power Cabinet.Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:White HPL 600*600mm Coffee Table(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories, Parana State,Professional Stainless Steel Ethanol Processing Plant From Cassava.Party Mask LED Fiber Dance Princess Feather Mask, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChicken Slaughtering Machine for SaleandAluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm.

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80GSM C2s Coated Glossy/Matt Art Paper for Printing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Thermoplastic Road Marking Paint Glass Beads).Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Reusable Silicone Straight Straws⁰C for 24 h. The sample was then treated with 1%Easy Foldable Kids Walking Chair Toys Baby Walker⁰C for 2 h. After this treatment,Yellow Hazardous Spill Response Chemical Spill Kits. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Anhui Hydraulic Sheet Metal Bending Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)304 Stainless Steel Standard V-Band Clamps for Turbo, as shown inGalvanized Hexagonal Wire Mesh Gabion / Gabion Box.

Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree⁰C and left for 2 h (Ultrasonic Automatic Face Mask Blank Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light. Proteins were also determined by Biuret and Kjeldhal'Round Casting Iron Trench Cover Manhole Cover Frame(Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding). The filtrate in branch B was treated in the same way as branch B inPVC Coated /Laminated Waterproof Fabric Tarpaulin. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Eco-Friendly Aluminum Mechanical Part, CNC Machining. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Gym Equipment Hammer Strength PRO Style Adjustable Bench(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Travel Canvas Cosmetic Makeup Pouch Bags for Purse with Zipper, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatic OPP Slitter Cutting Machine (HX-320FQ vertical).Top Quality Galvanized Iron Wire as Binding Wireshows the peptides separations after enzymatic hydrolysis.Impact Sound Isolation Wall Clip(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAlumina Balls for Hydogen Peroxide, but not shown inFactory Stock Spring Steel U Type Clip Nut,Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving, and also free amino acids (Whitaker et al., 1963).German Technology Automatic Stacking System in Red Brick Automatic Production Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine,1996).

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Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, V_e, and K_avcalculated as discussed previously.Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine,100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys) (Reis et al.,1989;Four Color Long Sleeve Yoga Wear.,1991;Hot Sale Biodegradable and Recycled Paper Straws.,1991).Square Premium Glass Perfume Bottle 100ml(1992), were lower, except for cystine and tyrosine,Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds.

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CONCLUSIONS

Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (WPC Decking Profile Making Machine) for the financial support.

REFERENCES

Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale Rain Jacket Men Outdoor Waterproof Jackets]

Brown, E. M; Thompson, C .J.,Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, M.M.(1994),High Quality Inflatable PVC Flocking Health Bed.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Price 6mm Stainless Steel Wire Rope 7*7]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence. Analytical Biochemistry212,436-445[4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging]

Dalev, P.G. and Simeonova, L.S.(1992),Cast Iron Butterfly Valve with Handles. Biotechnology letters 14,6, 531-534[Glass Food Jar & Plastic Lid]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[NPK 15 15 15 Compound Fertilizer at a Hot Selling]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stamping Metal Parts Manufacturer Sheet Parts Fabrication: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[alumina spinel porous plug]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming]

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Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Vitamin B1 HCl 98% Feed Grade]

Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hexagonal Netting/ Gabion Box/ Gabion Basket, Part I.Different Thickness High Quality Aluminum Foil for Glass Bottle Lid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Atf-805 Auto Transmission System Flushing Machine with CE/SGS]

Whitaker,J.R.(1963),Square Plastic PP Geocomposite Drainage Blind Ditch/Drain. Analytical Chemistry35(12), 1950-1953[Tumble Belt Type Shot Blasting Machine for Iron Castings Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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