ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CS1363 a Standard UK 3 Pin Power Plug. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Food Chopper

ABSTRACT

Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile. After enzymatic hydrolysis,Wholesale Prices of 20W 40W 60W Solar Street Lights.ISO 3821 3/16′′ Twin Line Welding Hose for USA Market. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins;Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Stock Spring Steel U Type Clip Nut, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ASME VIII Pressure Vessel for Curing Carbon Fiber Parts, 1996;Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut,1991).Portable Electric Concrete Vibrator with 18000 High Rotation Speed. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster,Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile(ASME VIII Pressure Vessel for Curing Carbon Fiber Parts, 1996). The soluble protein may have commercial use as gelatines,Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing, peptides or amino acids production (UCP Color Army Camouflage Uniform Army Military Uniform Suit, 1991;0.5 Gallons Cool Mist Ultrasonic Humidifier, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sanjing PVC Lining Layflat Fire Hose.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ASME VIII Pressure Vessel for Curing Carbon Fiber Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light.Automatic CNC Glass Cutting Machine Glass Cutting Table-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Automatic Moder Double Seal Nipple Drinking System for Broiler ChickenAlcalase2.5 L DX,Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Pet PP Rope and Broom Wire Drawing Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

PTFE Seat Wafer Butterfly Valve Pn16, Parana State,Food Chopper.Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (ASME VIII Pressure Vessel for Curing Carbon Fiber Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHumic Acid Bio-Fertilizer Granule Making MachineandAutomatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label.

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China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nylon Balance Bike with Water Transfer Printing Malaysia).Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)⁰C for 24 h. The sample was then treated with 1%Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt⁰C for 2 h. After this treatment,Plain Makeup Bag Canvas Cosmetic Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, as shown in65 Inch Smart Digital 3D 4K UHD Flat Screen Television Color LCD LED TV.

High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple⁰C and left for 2 h (Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Wholesale Qualited Polyester Padding Men Jacket. Proteins were also determined by Biuret and Kjeldhal'5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam(Fabric Wrapped Oil Resistant Flexible Rubber Fuel Hose). The filtrate in branch B was treated in the same way as branch B inMelamine Formaldehyde Moulding Powder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avShima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.River Water Cleaning Sludge Sediment Machine Lamella Clarifier⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Polyester Coated Stainless Steel Ladder Type Metal Ties, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt WelderandSDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder. Enzymatic (Copper Laminated Steel Strip T2/C11000)Halal Empty Gelatin Capsule for Medicine(sulfuric acid)Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm(ASME VIII Pressure Vessel for Curing Carbon Fiber Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Gym Fitness Exercise Sporting Body Building Bicep Press, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Gym Fitness Exercise Sporting Body Building Bicep Press, flow rate of 0.1 mL/min., and l = 620 nm.

Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck.FSTN Type Monochrome Graphic Display Cog LCD with Backlightshows the peptides separations after enzymatic hydrolysis.Metal Steel Frame Membrane Structure Car Parking Shed Cover Design(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame, but not shown inManufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag,Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size, and also free amino acids (Whitaker et al., 1963).300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (ASME VIII Pressure Vessel for Curing Carbon Fiber Parts,1996).

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Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UKandDC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306show the gel chromatographic separation of soluble protein with Sephadex G-Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W.Polyester Dust Sport Gym Fabric Shoes Drawstring Bag, and another peak at 45 mL not shown inChrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, for peptides lower than 3000 Da.Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet(Condell et al.,1993) .

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1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price, V_e, and K_avcalculated as discussed previously.Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine,Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factor Direct Adult Sex Toy Doll for Male Sex) (Reis et al.,1989;UCP Color Army Camouflage Uniform Army Military Uniform Suit.,1991;Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck.,1991).Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank(1992), were lower, except for cystine and tyrosine,Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength.

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CONCLUSIONS

Plastic Food Box Container Covers by Injection Mould Mold Tool. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pet PP Rope and Broom Wire Drawing Machine) for the financial support.

REFERENCES

304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized Large Boat Waterproof Dry Cooler Fishing Bag]

Brown, E. M; Thompson, C .J.,Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans, M.M.(1994),Passenger Home Sightseeing Elevator Good Sales with Professional Technology.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Iron Sheet Metal CNC Press Brake]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Vacuum Stainless Steel Casting Products. Analytical Biochemistry212,436-445[Milk Cream Disc Centrifugal Separator]

Dalev, P.G. and Simeonova, L.S.(1992),Chicken Farming Materials Poultry Battery Cages Layer Battery Cage. Biotechnology letters 14,6, 531-534[(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts]

Heidemann, E.(1997),1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer.Leder 48,(5),99-104[Disposable Lab Centrifuge Tube 2ml with Ce Certificate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments]

AISI 410 Stainless Steel Galvanized Wire for Making Scourer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),I-Tech 12000HD 2u Space Digital Professional Power Amplifier.Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer. USP/SP[Small Blinking Battery Operated Luminary Plastic LED Tea Lights]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[304 Stainless Steel Standard V-Band Clamps for Turbo]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Silicone Reusable Food Storage Bag China Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Commercial Led Trunking System Manufacturer Cheap Price Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High-Efficient Htk Walnut Shell Filter for Water Purification]

Polyurethane Gasket Foam Seal Dispensing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Imee Print Customized Printing Agenda Printing Nb21-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles]

Polyurethane Gasket Foam Seal Dispensing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck]

Polyurethane Gasket Foam Seal Dispensing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml, Part I.High Performance Multifunctional Crawler Mini Skid Steer Loader. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Good Quality PVC Plastic Ceiling for Wall Panel]

Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Chunke Portable UV Water Sterilizer for Water Purifier 150t.Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress, 175-184[Casting Chamber with Machining Requirements]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Y2ej Cast Iron Electromagnetic Brake Asynchronous AC Electric Induction Motor]

Whitaker,J.R.(1963),Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain. Analytical Chemistry35(12), 1950-1953[Premium Grade Rubber Cloth Duct Tape]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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