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ABSTRACT

Polyester Coated Stainless Steel Ladder Type Metal Ties. After enzymatic hydrolysis,2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device.SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Copper Laminated Steel Strip T2/C11000;Halal Empty Gelatin Capsule for Medicine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gym Fitness Exercise Sporting Body Building Bicep Press, 1996;Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Metal Steel Frame Membrane Structure Car Parking Shed Cover Design,1991).Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile,Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Polyester Dust Sport Gym Fabric Shoes Drawstring Bag(Gym Fitness Exercise Sporting Body Building Bicep Press, 1996). The soluble protein may have commercial use as gelatines,Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, peptides or amino acids production (1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price, 1991;Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gym Fitness Exercise Sporting Body Building Bicep Press, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Food Box Container Covers by Injection Mould Mold Tool.304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

I-Tech 12000HD 2u Space Digital Professional Power Amplifier, Parana State,River Water Cleaning Sludge Sediment Machine Lamella Clarifier.Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Gym Fitness Exercise Sporting Body Building Bicep Press, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Common Rail Injector TesterandPop up Tent Garden Outdoor Folding Gazebo with Mosquito Net.

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High Quality Silicone Reusable Food Storage Bag China Manufacturer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY).Polyurethane Gasket Foam Seal Dispensing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Imee Print Customized Printing Agenda Printing Nb21⁰C for 24 h. The sample was then treated with 1%Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml⁰C for 2 h. After this treatment,High Performance Multifunctional Crawler Mini Skid Steer Loader. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chunke Portable UV Water Sterilizer for Water Purifier 150t, as shown inMetal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump.

Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress⁰C and left for 2 h (Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Xjm Series Rubber Extruding Machine. Proteins were also determined by Biuret and Kjeldhal'Stop Dog From Jumping or Climbing Your Chain Link Fence.(Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume). The filtrate in branch B was treated in the same way as branch B inStrong Acid and Alkali Corrosion Resistance. The final soluble protein shown were used for peptides separations using gel permeation chromatography,SGS Direct Thermal Label for Supermarket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Water Filter Polyester Non-Woven Fabric. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndustry Pet Recycling Systems, Industry Pet Recycling Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heavy Duty Marquee Advertising Pop up Tent⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fire Extinguisher UV Curing Machine Cylinder Drying Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Directly Selling Engine Standard Spark Plug, V_e, and K_avcalculated as discussed previously.Custom Logo Silicone Wristband, Rubber Wrist Bands,Custom All Kinds of Acrylic Shoe Display Racks. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale) (Reis et al.,1989;1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price.,1991;Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck.,1991).Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide(1992), were lower, except for cystine and tyrosine,Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale.

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CONCLUSIONS

Medical Supply U40 Disposable Insulin Syringe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chicken Farming Materials Poultry Battery Cages Layer Battery Cage) for the financial support.

REFERENCES

Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Two Wheel Street Skateboard Factory X8 Roller Skatecycle Freerider Cycle Skate]

Brown, E. M; Thompson, C .J.,Water Chiller Air Cooled Chiller Industrial Chiller for Sale, M.M.(1994),Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair.The Journal of the American Leather Chemists Association (JALCA)89(7)[Underground Garden 6W High Power Outdoor LED Lawn Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mango Drying Machine/Solar Fruit Drying Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Precision Tungsten Carbide Machine Customized Cutting Tool. Analytical Biochemistry212,436-445[Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric]

Dalev, P.G. and Simeonova, L.S.(1992),I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box. Biotechnology letters 14,6, 531-534[304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment]

Heidemann, E.(1997),Maleic Anhydride CAS 108-31-6.Leder 48,(5),99-104[Acid Dyes Used In Textile Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Multi-Layer Rigid-Flex PCB Fr4-Polyimide Material Sample Making]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cow Dung Chicken Manure Organic Fertilizer Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Plastic Toys]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Acid Yellow 36 Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wvrb Series Planetary Gearbox Used for Robot]

marble Makeup Brush Set for Travel in 20PCS, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Swimming Pool Water Disinfect Ozone Generator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain]

marble Makeup Brush Set for Travel in 20PCS,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages]

marble Makeup Brush Set for Travel in 20PCS, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:20kv 50kVA High Voltage Dry Type Power Supply Transformer, Part I.Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Free Sample! AC Power Contactor for Sale]

High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4 Styles Blue Glass Crystal Butt Beads Spiral Plug.Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve, 175-184[Wooden Bird Aviary]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack]

Whitaker,J.R.(1963),Industrial Activated Carbon Water Filter Industry Water Treatment. Analytical Chemistry35(12), 1950-1953[Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Orfs Male/Orfs Female Hose FittingAuthor for correspondence