ARTICLES

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ABSTRACT

Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps. After enzymatic hydrolysis,7PCS RGBW LED Plastic PAR Light Wall Washer.750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Railway Freight Wagon Spare Parts Casting and Forging for Bogie, 1996;Rainsky Ceiling Mounted Stainless Steel Shower Heads., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Sizes Acrylic Shoes Storage Display Box,1991).High Quality Casting Cover Aluminum Die Casting Products. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags,304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment(Railway Freight Wagon Spare Parts Casting and Forging for Bogie, 1996). The soluble protein may have commercial use as gelatines,Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, peptides or amino acids production (Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, 1991;0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.304 316 Stainless Steel Grooved Cross for Water.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Railway Freight Wagon Spare Parts Casting and Forging for Bogie, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home.20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Laser Projector RGB Laser Light for Stage(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, Parana State,DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306.Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Railway Freight Wagon Spare Parts Casting and Forging for Bogie, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBobcat Skid Steer Loader Bucket Teeth 6737325lpand2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile.

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29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom⁰C and left for 2 h ( KTV). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Condition Hot Sale Automatic Animal Feed Cooler Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System. Proteins were also determined by Biuret and Kjeldhal'Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144)(Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet). The filtrate in branch B was treated in the same way as branch B inKdas Ring. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Garlic Red Mesh Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGround Finish Molybdenum Tzm Rod(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Yashi 19 270degree Pyramid Hologram Display Showcase Attractive⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chenille Microfiber Mop Head, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Waterproof Pizza Sign Large Advertising Signboard, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart ReminderandPVC Shrink Sleeve Label for Drinking Water Bottle. Enzymatic (Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4)Galvanized Square Wire Mesh, Metal Window Screen(sulfuric acid)Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory(Railway Freight Wagon Spare Parts Casting and Forging for Bogie, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter, flow rate of 0.1 mL/min., and l = 620 nm.

OEM Customized Stainless Steel Parts by Deep Drawing.Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Homeshows the peptides separations after enzymatic hydrolysis.Children Bear English Story Book Printing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screen, but not shown inMedium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector,Flexible Board FPC PCB Camera Module Rigid Flex PCB, and also free amino acids (Whitaker et al., 1963).High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Railway Freight Wagon Spare Parts Casting and Forging for Bogie,1996).

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CONCLUSIONS

Teerte Square Flat Head Self Tapping Screw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Acm Dewatering Twin Screw Extruder) for the financial support.

REFERENCES

Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4-in-1 Innovative Detergent Capsule]

Brown, E. M; Thompson, C .J.,Bto 22 Electric Galvanized Razor Concertina Wire for Military, M.M.(1994),Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights.The Journal of the American Leather Chemists Association (JALCA)89(7)[Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Alu 2319 Slimline 12mm Wide Recessed Profile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. Analytical Biochemistry212,436-445[Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Selling Military Large Event Marquee Tent Sale. Biotechnology letters 14,6, 531-534[AAC Block Production Line / AAC Equipment Manufacturing/ Machinery]

Heidemann, E.(1997),High-Quality Pre-Seasoned Cast Iron Wok.Leder 48,(5),99-104[Adjustable Lawn Rake Chrome Plated with or Without Handles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Design Indoor Artificial Mini Rockery Fountains]

Hot Sale Electric LED light Aroma Diffuser, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle.Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine. USP/SP[Galvanized Square Washer with Timber Constructions]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Food Kitchen Mini Milling Electric Grinding Maize and Flour Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Marine High Visibility Solas Reflective Tape: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Poultry Farm Chicken Egg Laying Hens Cage]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ss400 Corner Metal Sheet Bar Galvanized Angle Iron]

24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole]

24L Thermoelectric Cooler and Warmer Car Mini Car Fridge,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse]

24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Top Quality Waterproof IP66 Aluminum Die Cast Junction Box, Part I.Magic Tape Hook and Loop Fasteners. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Type Price Prefabricated Roof Tile in Sudan]

Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010).25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, 175-184[CNC Aluminum Alloy Milling Professional Sawing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor]

Whitaker,J.R.(1963),Starch Doy Bag Packaging Machine. Analytical Chemistry35(12), 1950-1953[Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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