ARTICLES

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ABSTRACT

12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope. After enzymatic hydrolysis,8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector.Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115;China Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Cross Recesed Flat Head Self Tapping Screw, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Our Perforated Aluminum & Solid Panel for New Office Wall Cladding, 1996;2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Kids Trucker Hat, Kids Mesh Cap,1991).Wd190V Handheld Full Body Scanner Hand Held Metal Detector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed,Hydraulic Orbital Valve Power Steering,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine(Our Perforated Aluminum & Solid Panel for New Office Wall Cladding, 1996). The soluble protein may have commercial use as gelatines,AISI 410 Stainless Steel Galvanized Wire for Making Scourer, peptides or amino acids production (CS1363 a Standard UK 3 Pin Power Plug, 1991;Food Chopper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Prices of 20W 40W 60W Solar Street Lights.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ISO 3821 3/16′′ Twin Line Welding Hose for USA Market. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Our Perforated Aluminum & Solid Panel for New Office Wall Cladding, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins.Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Stock Spring Steel U Type Clip Nut(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring).Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%UCP Color Army Camouflage Uniform Army Military Uniform Suit⁰C for 24 h. The sample was then treated with 1%0.5 Gallons Cool Mist Ultrasonic Humidifier⁰C for 2 h. After this treatment,Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sanjing PVC Lining Layflat Fire Hose's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar, as shown in4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine.

China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light⁰C and left for 2 h (I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic CNC Glass Cutting Machine Glass Cutting Table. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory. Proteins were also determined by Biuret and Kjeldhal'Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken(Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck). The filtrate in branch B was treated in the same way as branch B inMercedes Air Suspension Front Shock Absorber. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pet PP Rope and Broom Wire Drawing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Skytone 4 Channels 3500W Each DJ Power AmplifierandAluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01). Enzymatic (Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt)Plain Makeup Bag Canvas Cosmetic Bag(sulfuric acid)14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow(Our Perforated Aluminum & Solid Panel for New Office Wall Cladding, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose, flow rate of 0.1 mL/min., and l = 620 nm.

High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple.Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machineshows the peptides separations after enzymatic hydrolysis.Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic L Bar Sealer Shrink Tunnel Machine, but not shown inCommon Rail Diesel Injector Nozzle Dlla153p977,Factory Wholesale Qualited Polyester Padding Men Jacket, and also free amino acids (Whitaker et al., 1963).5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Our Perforated Aluminum & Solid Panel for New Office Wall Cladding,1996).

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Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat, V_e, and K_avcalculated as discussed previously.River Water Cleaning Sludge Sediment Machine Lamella Clarifier,Polyester Coated Stainless Steel Ladder Type Metal Ties. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board) (Reis et al.,1989;CS1363 a Standard UK 3 Pin Power Plug.,1991;2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone.,1991).2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device(1992), were lower, except for cystine and tyrosine,SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder.

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CONCLUSIONS

Copper Laminated Steel Strip T2/C11000. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut) for the financial support.

REFERENCES

Halal Empty Gelatin Capsule for Medicine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Large Vertical Granule Packing Machine (KENO-F103)]

Brown, E. M; Thompson, C .J.,Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, M.M.(1994),Gym Fitness Exercise Sporting Body Building Bicep Press.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Design Plush Panda Animal Slippers Comfort Foam Support by]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck. Analytical Biochemistry212,436-445[5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building]

Dalev, P.G. and Simeonova, L.S.(1992),Metal Steel Frame Membrane Structure Car Parking Shed Cover Design. Biotechnology letters 14,6, 531-534[Halal Empty Gelatin Capsule for Medicine]

Heidemann, E.(1997),Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size.Leder 48,(5),99-104[Sheet Metal Stamping Parts Cutting and Bending Machine Fabrication Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Mushroom Rround Head Solid Rivet (Round head)]

Alu 2319 Slimline 12mm Wide Recessed Profile, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile.Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W. USP/SP[22*30mm 12SMD 5050 LED Reading Light for Car]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[DIN127 Stainless Steel Spring Lock Washer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Polyester Dust Sport Gym Fabric Shoes Drawstring Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plough Share Point with Tungsten Carbide]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM]

Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wet Wire Drawing Machine for High Carbon Mattress Spring Wire]

Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw]

Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, Part I.Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord]

Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength.Plastic Food Box Container Covers by Injection Mould Mold Tool, 175-184[CNC Router Woodworking Engraving Cutting Machine for Acrylic/Wood/Plastic/Aluminum]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Valve Bag Dry Mortar Bagging Machine]

Whitaker,J.R.(1963),304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment. Analytical Chemistry35(12), 1950-1953[Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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